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Magic Angle Spinning (MAS) NMR for Structure Determination in Photosynthesis

  • Huub J. M. de Groot
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 26)

Using solid-state nuclear magnetic resonance (NMR), structure and its underlying details, such as the rigidity, order, dynamics or electrostatic heterogeneity of the large membrane protein complexes involved in photosynthesis can be resolved at specifi c spots with a resolution well beyond the capabilities of X-ray and other diffraction methods. Following a brief explanation of the theoretical background of the magic angle spinning NMR methods, applications are presented involving specifi c and extensive labeling methods. The specifi c labeling methods provide access to the structure of the large reaction center protein complexes involved in photosynthesis and can be used to study and resolve details of the structure such as rigidity or dynamics of cofactors, H-bonding of the quinones, ground state charge effects on histidines and ligation characteristics of the Ca2+ binding site in Photosystem II. In this way spots of physical frustration in the spatial, protonic and electronic structure of the ground state that are important for the biological mechanisms can be identifi ed. In addition, the first de novo structure determination of systems comprised of smaller building blocks was made for the chlorosomes in the green photosynthetic bacterium Chlorobium tepidum. When the MAS data for the chlorosomes are compared with data collected from the isolated labeled BChl c aggregated in n-hexane, it can be shown that the major component of the MAS signals in the chlorosomes is from aggregated BChl c and a bilayer tube model for the structure was obtained from MAS NMR correlation spectroscopy and molecular modeling. In addition molecular mechanisms steering the suprastructure were identifi ed by model studies. They provide a view on evolutionary selection and may be of interest for future design of artifi cial photosynthesis structures. Finally it is shown how pattern labeling of the LH2 protein leads to sequence specifi c assignments in a strategy that can be used to resolve structure of small membrane proteins and complexes.

Keywords

Nuclear Magnetic Resonance Nuclear Magnetic Resonance Spectroscopy Magic Angle Spin Photosynthetic Reaction Center Nuclear Magnetic Resonance Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer Science+Business Media B.V 2008

Authors and Affiliations

  • Huub J. M. de Groot
    • 1
  1. 1.Leiden Institute of ChemistryLeiden UniversityNetherlands

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