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Optical Spectroscopy of Individual Light-Harvesting Complexes

  • Thijs J. Aartsma
  • Jürgen Köhler
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 26)

In this contribution we present an overview of the results of detailed spectroscopic investigations of individual light-harvesting complexes of photosynthetic purple bacteria at 1.2 K. By applying single-molecule detection techniques the properties of the electronically excited states of the assemblies of bacteriochlorophyll a (BChl a) pigment molecules in the individual complexes are revealed, without ensemble averaging. The results show that the excited states of the B800 ring of pigments in LH2 are largely localized on individual BChl a molecules, although accidental degeneracy gives rise to an excitonic character of the B800 excited states. In contrast, the absorption of a photon by the B850 ring can be consistently described in terms of an excitation that is more or less delocalized over the ring. This property is believed to contribute to the high effi ciency of energy transfer in these photosynthetic complexes. The analysis of the spectra in terms of disorder is reviewed in some detail. The results of LH2 are compared with those of similar complexes, LH3 and low-light LH2. In addition, we consider the fl uorescence spectra of individual LH2 and LH3 complexes.

Keywords

Purple Bacterium Exciton State Rhodobacter Sphaeroides Site Energy Spectral Diffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V 2008

Authors and Affiliations

  • Thijs J. Aartsma
    • 1
  • Jürgen Köhler
    • 2
  1. 1.Department of BiophysicsLeiden UniversityNetherlands
  2. 2.Lehrstuhl für Experimentalphysik IVUniversität BayreuthGermany

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