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(Sub)-Picosecond Spectral Evolution of Fluorescence Studied with a Synchroscan Streak-Camera System and Target Analysis

  • Ivo H. M. Van Stokkum
  • Bart Van Oort
  • Frank Van Mourik
  • Bas Gobets
  • Herbert Van Amerongen
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 26)

A synchroscan streak camera in combination with a spectrograph can simultaneously record temporal dynamics and wavelength of fl uorescence representable as an image with time and wavelength along the axes. The instrument response width is about 1% of the time range (of typically 200 ps to 2 ns). The spectral window of 250 nm may lie between 250 and 850 nm. Such spectrotemporal measurements using low excitation intensities have become routine. Sophisticated data analysis methods are mandatory to extract meaningful physicochemical parameters from the wealth of information contained in the streak image. In target analysis a kinetic scheme is used in combination with assumptions on the spectra of the species to describe the system. In this chapter the principals of operation of a streak-camera setup are described, along with the fundamental and technical limitations that one encounters. The correction and calibration steps that are needed as well as data processing and analysis are discussed. Several case studies of bioluminescence are presented, with a particularly in-depth analysis of trimeric Photosystem I core particles of the cyanobacterium Spirulina platensis.

Keywords

Singular Value Decomposition Singular Vector Streak Camera Purple Membrane Spectral Evolution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V 2008

Authors and Affiliations

  • Ivo H. M. Van Stokkum
    • 1
  • Bart Van Oort
    • 2
  • Frank Van Mourik
    • 3
  • Bas Gobets
    • 4
  • Herbert Van Amerongen
    • 2
  1. 1.Department of Physics and AstronomyVrije UniversiteitNetherlands
  2. 2.Laboratory of BiophysicsWageningen UniversityNetherlands
  3. 3.École Polytechnique Fédérale de Lau sanneInstitut de Sciences et Ingéniérie ChimiquesSwitzerland
  4. 4.Image Science InstituteUniversity Medical Center UtrechtNetherlands

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