In this chapter we describe how femtosecond time-resolved infrared spectroscopy is useful for the study of the dynamics of pigment-protein complexes, and what the technical requirements are to perform such experiments. We further discuss a few examples of experiments performed on photosynthetic complexes in more detail.
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References
Barth A and Zscherp C (2002) What vibrations tell us about proteins. Q Rev Biophys 35: 369-430
Bredenbeck J, Helbing J, Kumita JR, Woolley GA and Hamm P (2005) α-Helix formation in a photoswitchable peptide tracked from picoseconds to microseconds by time-resolved IR spectroscopy. Proc Natl Acad Sci USA 102: 2379-2384
Breton J, Hienerwadel R and Nabedryk E (1997) Hydrogen bonding to the primary electron donor in reaction centers from Rb. sphaeroides: FTIR characterization of a series of mutants at residue M160. In: Carmona P (ed) Spectroscopy of Biological Molecules: Modern Trends, pp 101-106. Kluwer Academic Publishers, Dordrecht
Cerullo G, Nisoli M and De Silvestri S (1997) Generation of 11 fs pulses tunable across the visible by optical parametric amplification. Appl Phys Lett 71: 3616-3618
Cerullo G, Nisoli M, Stagira S and De Silvestri S (1998) Sub-8-fs pulses from an ultrabroadband optical parametric amplifier in the visible. Opt Lett 23: 1283-1285
de Weerd FL, van Stokkum IHM, van Amerongen H, Dekker JP and van Grondelle R (2002) Pathways for energy transfer in the core light-harvesting complexes CP43 and CP47 of Photosystem II. Biophys J 82: 1586-1597
Demirdoven N, Cheatum CM, Chung HS, Khalil M, Knoester J and Tokmakoff A (2004) Two-dimensional infrared spectroscopy of antiparallel beta-sheet secondary structure. J Am Chem Soc 126: 7981-7990
Diller R, Iannone M, Cowen BR, Maiti S, Bogomolni RA and Hochstrasser RM (1992) Picosecond dynamics of bacteriorhodopsin, probed by time-resolved infrared-spectroscopy. Biochemistry 31: 5567-5572
Dreyer J, Moran AM and Mukamel S (2003) Coherent threepulse spectroscopy of coupled vibrations in a rigid dipeptide: Density functional theory simulations. J Phys Chem B 107: 5967-5985
Eaves JD, Loparo JJ, Fecko CJ, Roberts ST, Tokmakoff A and Geissler PL (2005) Hydrogen bonds in liquid water are broken only fleetingly. Proc Natl Acad Sci USA 102: 13019-13022
Ferreira KN, Iverson TM, Maghlaoui K, Barber J and Iwata S (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303: 1831-1838
Groma GI, Colonna A, Lambry JC, Petrich JW, Varo G, Joffre M, Vos MH and Martin JL (2004) Resonant optical rectification in bacteriorhodopsin. Proc Natl Acad Sci USA 101: 7971-7975
Groot ML, Vos MH, Schlichting I, van Mourik F, Joffre M, Lambry JC and Martin JL (2002) Coherent infrared emission from myoglobin crystals: An electric field measurement. Proc Natl Acad Sci USA 99: 1323-1328
Groot ML, van Wilderen L, Larsen DS, van der Horst MA, van Stokkum IHM, Hellingwerf KJ and van Grondelle R (2003) Initial steps of signal generation in photoactive yellow protein revealed with femtosecond mid-infrared spectroscopy. Biochemistry 42: 10054-10059
Groot ML, Breton J, van Wilderen L, Dekker JP and van Grondelle R (2004) Femtosecond visible/visible and visible/mid-IR pump-probe study of the Photosystem II core antenna complex CP47. J Phys Chem B 108: 8001-8006
Groot ML, Pawlowicz NP, van Wilderen L, Breton J, van Stokkum IHM and van Grondelle R (2005) Initial electron donor and acceptor in isolated Photosystem II reaction centers identified with femtosecond mid-IR spectroscopy. Proc Natl Acad Sci USA 102: 13087-13092
Hahn S, Kim SS, Lee C and Cho M (2005) Characteristic twodimensional IR spectroscopic features of antiparallel and parallel beta-sheet polypeptides: Simulation studies. J Chem Phys 123: 084905 (10 pages).
Hamacher E, Kruip J, Rögner M and Mäntele W (1996) Characterization of the primary electron donor of Photosystem I, P700, by electrochemistry and Fourier-transform infrared (FTIR) difference spectroscopy. Spectrochim Acta A 52: 107-121
Hamm P (1995) Coherent effects in femtosecond infrared-spectroscopy. Chem Phys 200: 415-429
Hamm P, Zurek M, Mäntele W, Meyer M, Scheer H and Zinth W (1995) Femtosecond infrared-spectroscopy of reaction centers from Rhodobacter sphaeroides between 1000 and 1800 cm−1. Proc Natl Acad Sci USA 92: 1826-1830
Hamm P, Lim MH and Hochstrasser RM (1998) Structure of the amide I band of peptides measured by femtosecond nonlinearinfrared spectroscopy. J Phys Chem B 102: 6123-6138
Hamm P, Kaindl RA and Stenger J (2000) Noise suppression in femtosecond mid-infrared light sources. Opt Lett 25: 1798-1800
Herbst J, Heyne K and Diller R (2002) Femtosecond infrared spectroscopy of bacteriorhodopsin chromophore isomerization. Science 297: 822-825
Hill JR, Tokmakoff A, Peterson KA, Sauter B, Zimdars D, Dlott DD and Fayer MD (1994) Vibrational dynamics of carbonmonoxide at the active-site of myoglobin — Picosecond infrared free-electron laser pump-probe experiments. J Phys Chem 98: 11213-11219
Hill JR, Dlott DD, Rella CW, Smith TI, Schwettman HA, Peterson KA, Kwok A, Rector KD and Fayer MD (1996) Ultrafast infrared spectroscopy in biomolecules: Active site dynamics of heme proteins. Biospectroscopy 2: 277-299
Joffre M, Hulin D, Migus A, Antonetti A, Laguillaume CBA, Peyghambarian N, Lindberg M and Koch SW (1988) Coherent effects in pump probe spectroscopy of excitons. Opt Lett 13: 276-278
Kim YS and Hochstrasser RM (2005) Chemical exchange 2D IR of hydrogen-bond making and breaking. Proc Natl Acad Sci USA 102: 11185-11190
Kotting C and Gerwert K (2005) Proteins in action monitored by time-resolved FTIR spectroscopy. Chem Phys Chem 6: 881-888
Lim M, Jackson TA and Anfinrud PA (1995a) Binding of CO to myoglobin from a heme pocket docking site to form nearly linear Fe-C-O. Science 269: 962-966
Lim MH, Jackson TA and Anfinrud PA (1995b) Midinfrared vibrational spectrum of CO after photodissociation from heme evidence for a ligand docking site in the heme pocket of hemoglobin and myoglobin. J Chem Phys 102: 4355-4366
Loll B, Kern J, Saenger W, Zouni A and Biesiadka J (2005) Towards complete cofactor arrangement in the 3.0 Ångström resolution structure of Photosystem II. Nature 438: 1040-1044
Maiti S, Cowen BR, Diller R, Iannone M, Moser CC, Dutton PL and Hochstrasser RM (1993) Picosecond infrared studies of the dynamics of the photosynthetic reaction center. Proc Natl Acad Sci USA 90: 5247-5251
Maiti S, Walker GC, Cowen BR, Pippenger R, Moser CC, Dutton PL and Hochstrasser RM (1994) Femtosecond coherent transient infrared-spectroscopy of reaction centers from Rhodobacter sphaeroides. Proc Natl Acad Sci USA 91: 10360-10364
Mäntele W (1996) Infrared and Fourier-Transform Infrared Spectroscopy. In: Amesz J and Hoff AJ (eds) Biophysical Techniques in Photosynthesis (Advances in Photosynthesis, Vol 3), pp 137-157. Kluwer Academic Publishers, Dordrecht
Nabedryk E, Andrianambinintsoa S, Berger G, Leonhard M, Mäntele W and Breton J (1990) Characterization of bonding interactions of the intermediary electron-acceptor in the reaction center of Photosystem-II by FTIR spectroscopy. Biochim Biophys Acta 1016: 49-54
Nabedryk E, Allen JP, Taguchi AKW, Williams JC, Woodbury NW and Breton J (1993) Fourier-transform infrared study of the primary electron-donor in chromatophores of Rhodobacter sphaeroides with reaction centers genetically-modified at residue M160 and residue L131. Biochemistry 32: 13879-13885
Nabedryk E, Breton J, Williams JC, Allen JP, Kuhn M and Lubitz W (1998) FTIR characterization of the primary electron donor in double mutants combining the heterodimer HL(M202) with the LH(L131), HF(L168), FH(M197), or LH(M160) mutations. Spectrochim Acta A 54: 1219-1230
Nabedryk E, Schulz C, Müh F, Lubitz W and Breton J (2000) Heterodimeric versus homodimeric structure of the primary electron donor in Rhodobacter sphaeroides reaction centers genetically modified at position M202. Photochem Photobiol 71: 582-588
Noguchi T, Tomo T and Kato C (2001) Triplet formation on a monomeric chlorophyll in the Photosystem II reaction center as studied by time-resolved infrared spectroscopy. Biochemistry 40: 2176-2185
Novoderezhkin VI, Palacios MA, van Amerongen H and van Grondelle R (2004) Energy-transfer dynamics in the LHCII complex of higher plants: Modified redfield approach. J Phys Chem B 108: 10363-10375
Rubtsov IV, Kumar K and Hochstrasser RM (2005) Dual-frequency 2D IR photon echo of a hydrogen bond. Chem Phys Lett 402: 439-443
Scheurer C and Mukamel S (2002) Infrared analogs of heteronuclear nuclear magnetic resonance coherence transfer experiments in peptides. J Chem Phys 116: 6803-6816
Shirakawa A, Sakane I and Kobayashi T (1998) Pulse-frontmatched optical parametric amplification for sub-10-fs pulse generation tunable in the visible and near infrared. Opt Lett 23: 1292-1294
Van Stokkum IHM, Larsen DS and Van Grondelle R (2004) Global and target analysis of time-resolved spectra. Biochim Biophys Acta 1657: 82-104
Ventalon C, Fraser JM, Vos MH, Alexandrou A, Martin JL and Joffre M (2004) Coherent vibrational climbing in carboxyhemoglobin. Proc Natl Acad Sci USA 101: 13216-13220
Venyaminov SY and Kalnin NN (1990) Quantitative IR spectrophotometry of peptide compounds in water (H2O) solutions 2. Amide absorption-bands of polypeptides and fibrous proteins in alpha-coil, beta-coil, and random coil conformations. Biopolymers 30: 1259-1271
Walker GC, Maiti S, Cowen BR, Moser CC, Dutton PL and Hochstrasser RM (1994) Time Resolution of electronic-transitions of photosynthetic reaction centers in the infrared. J Phys Chem 98: 5778-5783
Wilhelm T, Piel J and Riedle E (1997) Sub-20-fs pulses tunable across the visible from a blue-pumped single-pass noncollinear parametric converter. Opt Lett 22: 1494-1496
Zouni A, Witt HT, Kern J, Fromme P, Krauss N, Saenger W and Orth P (2001) Crystal structure of Photosystem II from Synechococcus elongatus at 3.8 Ångström resolution. Nature 409: 739-743
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Groot, M.L., Van Grondelle, R. (2008). Femtosecond Time-Resolved Infrared Spectroscopy. In: Aartsma, T.J., Matysik, J. (eds) Biophysical Techniques in Photosynthesis. Advances in Photosynthesis and Respiration, vol 26. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8250-4_10
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DOI: https://doi.org/10.1007/978-1-4020-8250-4_10
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