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Long-Term Retention of Fluorescent Quantum Dots In Vivo

  • Conference paper
Nanomaterials for Application in Medicine and Biology

Part of the book series: NATO Science for Peace and Security Series ((NAPSB))

Abstract

Quantum dots that emit in the near-infrared can be used in vivo to follow circulation, to target the reticuloendothelial system, and to map lymphatic drainage from normal tissues and tumors. We have explored the role of surface charge and passivation by polyethylene glycol in determining circulating lifetimes and sites of deposition. Use of long polyethylene glycol polymers increases circulating lifetime. Changing surface charge can partially direct quantum dots to the liver and spleen, or the lymph nodes. Quantum dots are cleared in the order liver > spleen > bone marrow > lymph nodes. Quantum dots retained by lymph nodes maintained fluorescence for two years, suggesting either that the coating is extremely stable or that some endosomes preserve quantum dot function. We also explored migration from tumors to sentinel lymph nodes using tumor models in mice; surface charge and size make little difference to transport from tumors. Antibody and Fab-conjugates of polymer-coated quantum dots failed to target tumors in vivo, probably because of size.

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Author information

Authors and Affiliations

  1. Molecular Biosensor and Imaging Center, Mellon Institute, Carnegie Mellon Unversity, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA

    Byron Ballou, Lauren A. Ernst, Susan Andreko, Marcel P. Eructiez, B. Christoffer Lagerholm & Alan S. Waggoner

  2. Department of Biological Sciences, Mellon Institute, Carnegie Mellon Unversity, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA

    Byron Ballou & Alan S. Waggoner

  3. Department of Chemistry, Mellon Institute, Carnegie Mellon Unversity, 4400 Fifth Avenue, Pittsburgh, PA, 15213, USA

    Marcel P. Eructiez

  4. Memphys, Physics Department, University of Southern Denmark, Campusvej 55, 5230, Odense, Denmark

    B. Christoffer Lagerholm

Authors
  1. Byron Ballou
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  2. Lauren A. Ernst
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  3. Susan Andreko
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  4. Marcel P. Eructiez
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  5. B. Christoffer Lagerholm
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  6. Alan S. Waggoner
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Editor information

Editors and Affiliations

  1. Center of advanced european studies and research (caesar), Bonn, Germany

    Michael Giersig

  2. Moscow State University, Moscow, Russia

    Gennady B. Khomutov

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Ballou, B., Ernst, L., Andreko, S., Eructiez, M., Lagerholm, B., Waggoner, A. (2008). Long-Term Retention of Fluorescent Quantum Dots In Vivo. In: Giersig, M., Khomutov, G.B. (eds) Nanomaterials for Application in Medicine and Biology. NATO Science for Peace and Security Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6829-4_11

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