Abstract
To elucidate the biosynthetic pathways of carotenoids, especially myxol 2′-fucosides in cyanobacteria, an Anabaena sp. PCC 7120 deletion mutant was analyzed that was proposed to be lacking GDP-fucose synthase (WcaG, All4826). The mutant contained polar carotenoid glycosides and also free myxol. Their glycoside moiety was identified to be rhamnose, isomer of fucose, by FD-MS and 1HNMR, instead of the usual fucose. This result suggests that in the mutant, fucose is not synthesized, and rhamnose is bound to myxol as a substrate by fucosyltransferase, so that all4826 is a functional gene for GDP-fucose synthase. We also identified a β-carotene hydroxylase, CrtR (Alr4009), in Anabaena 7120 that catalyzed deoxymyxol to myxol.
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Mochimaru, M., Masukawa, H., Maoka, T., Takaichi, S. (2008). Functional Identification of GDP-Fucose Synthase Gene in Anabaena sp. PCC 7120. In: Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B. (eds) Photosynthesis. Energy from the Sun. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6709-9_75
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DOI: https://doi.org/10.1007/978-1-4020-6709-9_75
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