Functional Identification of GDP-Fucose Synthase Gene in Anabaena sp. PCC 7120

  • Mari Mochimaru
  • Hajime Masukawa
  • Takashi Maoka
  • Shinichi Takaichi
Conference paper

Abstract

To elucidate the biosynthetic pathways of carotenoids, especially myxol 2′-fucosides in cyanobacteria, an Anabaena sp. PCC 7120 deletion mutant was analyzed that was proposed to be lacking GDP-fucose synthase (WcaG, All4826). The mutant contained polar carotenoid glycosides and also free myxol. Their glycoside moiety was identified to be rhamnose, isomer of fucose, by FD-MS and 1HNMR, instead of the usual fucose. This result suggests that in the mutant, fucose is not synthesized, and rhamnose is bound to myxol as a substrate by fucosyltransferase, so that all4826 is a functional gene for GDP-fucose synthase. We also identified a β-carotene hydroxylase, CrtR (Alr4009), in Anabaena 7120 that catalyzed deoxymyxol to myxol.

Keywords

Carotenoid cyanobacteria Anabaena myxol wcaG crtR 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Mari Mochimaru
    • 1
  • Hajime Masukawa
    • 2
  • Takashi Maoka
    • 3
  • Shinichi Takaichi
    • 4
  1. 1.Department of Natural SciencesKomazawa UniversityTokyoJapan
  2. 2.Department of Biological SciencesKanagawa UniversityHiratsukaJapan
  3. 3.Research Institute for Production DevelopmentKyotoJapan
  4. 4.Department of BiologyNippon Medical SchoolKawasakiJapan

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