Investigation of Non-photochemical Processes in Photosynthetic Bacteria and Higher Plants Using Interference of Coherent Radiation – A Novel Approach

  • Karel Rohacek
  • Miroslav Kloz
  • David Bina
  • Frantisek Batysta
  • Frantisek Vacha

Abstract

Photosynthetic Light Energy Utilisation Is Accompanied With The Ph-Gradient Formation, O2 Production, Co2 Fixation, Heat Propagation And Other Processes, Which Result In Dynamic Changes In A Volume Of Chloroplasts, Whole Cells Or Plant Tissue. Here, We Present A New Concept, In Which The Volume Changes In Chloroplasts, Photosynthetic Bacteria, And Plant Leaves Are Measured Using Interference Of Coherent Light Beams Produced By A Hene Laser (ɚ 632.8 Nm). The Aim And Main Advantage Of This Interferometric Method Applied Newly To Photosynthesis Research Is The Possibility To Quantify The Non-Photochemical Processes In Photosynthetic Samples Measured In Vivo Using The Parallel Recording Of Interferograms And Chlorophyll A Fluorescence Induction Kinetics. In This Way, The Heat Production In Bacteria (Rhodobacter Sphaeroides) Treated With A Gramicidin, As Well As The O2 Evolution, Co2 Uptake And Transverse Dilatations In Leaves Of Bean (Phaseolus Vulgaris) Stressed By The Excessive Radiation Were Studied. We Found That The Heat Production Was Enhanced By Approximately 20% In Stressed Samples. Using Interferometry, We Have Also Observed Pronounced Transverse Dilatations In A Leaf Tissue Of Bean During Photosynthesis Caused Probably By Transpiration.

Keywords

Fluorescence interference laser nonphotochemistry photoinhibition photosystem II 

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References

  1. Buschmann C, Prehn H (1990) Photoacoustic spectroscopy - photoacoustic and photothermal effects. In: Linskens HF, Jackson JF (eds) Modern Methods of Plant Analysis. Springer, Berlin, pp 148-180.Google Scholar
  2. Hariharan P (2003) Optical Interferometry. Academic, Elsevier, London.Google Scholar
  3. Hariharan P (2007) Basics of Interferometry. Academic, Elsevier, London.Google Scholar
  4. Malkin S (1996) The photoacoustic method in photosynthesis - monitoring and analysis of phenomena which lead to pressure changes following light excitation. In: Amesz J, Hoff AJ (eds) Biophysical Techniques in Photosynthesis. Kluwer, Dordrecht, The Netherlands, pp 191-206.Google Scholar
  5. Robinson SP, Portis AR (1988) Involvement of stromal ATP in the light activation of Ribulose-1,5-bisphosphate carboxylase oxygenase in intact isolated chloroplasts. Plant Physiol 86:293-298.PubMedCrossRefGoogle Scholar
  6. Rohacek K, Bartak M (1999) Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. Photosynthetica 37:339-363.CrossRefGoogle Scholar
  7. Tabrizi H, Shinner K, Spors J, Hansen UP (1998) Deconvolution of three components of the photoacoustic signal by curve fitting and the relationship of CO2uptake to proton fluxes. Photosynth Res 57:101-115.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Karel Rohacek
    • 1
  • Miroslav Kloz
    • 2
  • David Bina
    • 1
    • 2
  • Frantisek Batysta
    • 3
  • Frantisek Vacha
    • 1
    • 2
  1. 1.Biology Centre of AS CR, p.r.i.Institute of Plant Molecular BiologyCeske BudejoviceCzech Republic
  2. 2.Faculty of BiologyUniversity of South BohemiaCeske BudejoviceCzech Republic
  3. 3.J.V. JirsikCeske BudejoviceCzech Republic

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