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Rubisco Activity Is Related To Photosystem I In Leaves

  • Hillar Eichelmann
  • Eero Talts
  • Vello Oja
  • Bahtijor Rasulov
  • Evi Padu
  • Agu Laisk
Conference paper

Abstract

Rubisco content and its in vivo activity (kcat), as well as PSI content were measured in leaves of sunflower, tobacco, potato and birch. The biological variation of k cat was wide, ranging from 1 to 6 s−1 at 22.5°C. Plotting kcat against (Rubisco sites/PSI) resulted in a reciprocally decreasing relationship. The data show that carbamylated Rubisco sites have variable average catalytic turnover rates in planta, regulated by Rubisco Activase in cooperation with a PSI-related factor.

Keywords

Leaves Rubisco Rubisco activase photosystem I 

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References

  1. Butz ND, Sharkey TD (1989) Activity ratios of ribulose-1,5-bisphosphate carboxylase accurately reflect car-bamylation ratios. Plant Physiol 89:735-739.PubMedCrossRefGoogle Scholar
  2. Cen Y-P, Sage RF (2005) The regulation of Rubisco activity in response to variation in temperature and atmospheric CO2 partial pressure in sweet potato. Plant Physiol 139:979-990.PubMedCrossRefGoogle Scholar
  3. He Z, von Caemmerer S, Hudson GS, Price DG, Badger MR, Andrews JT (1997) Ribulose-1,5-bisphosphate carboxylase/oxygenase activase deficiency delays senescence of ribulose-1,5-bisphosphate carboxy-lase/oxygenase but progressively impairs its catay-sis during tobacco leaf development. Plant Physiol 115:1569-1580.PubMedCrossRefGoogle Scholar
  4. Laisk A, Oja V (1998) Dynamic gas exchange of leaf pho-tosynthesis. Measurement and Interpretation. CSIRO, Canberra, Australia.Google Scholar
  5. Laisk A, Oja V, Rasulov B, Rämma H, Eichelmann H, Kasparova I, Pettai H, Padu E, Vapaavuori E (2002) A computer-operated routine of gas exchange and optical measurements to diagnose photosynthetic apparatus in leaves. Plant Cell Environ 25:923-943.CrossRefGoogle Scholar
  6. Oja V, Bichele I, Hüve K, Rasulov B, Laisk A (2004) Reductive titration of photosystem I and differential extinction coefficient of P700+ at 810-950 nm in leaves. Biochim Biophys Acta 1658:225-234.PubMedCrossRefGoogle Scholar
  7. Parry MA, Andralojc PJ, Mitchell RAC, Madgwick PJ, Keys AJ (2003) Manipulation of Rubisco: the amount, activ-ity, function and regulation. J Exp Bot 54:1321-1333.PubMedCrossRefGoogle Scholar
  8. Portis AR (2003) Rubisco activase - Rubisco’s catalytic chaperone. Photosynth Res 75:11-27.PubMedCrossRefGoogle Scholar
  9. Ruuska SA, Andrews TJ, Badger MR, Price GD, von Caemmerer S (2000) The role of chloroplast electron transport and metabolites in modulating Rubisco activ-ity in tobacco. Insights from transgenic plants with reduced amounts of cytochrome b/f complex or glyc-eraldehyde 3-phosphate dehydrogenase. Plant Physiol 122:491-504.PubMedCrossRefGoogle Scholar
  10. von Caemmerer S, Evans JR, Hudson GS, Andrews TJ (1994) The kinetics of ribulose-1,5-bisphosphate carboxylase/oxygenase in vivo inferred from meas-urements of photosynthesis in leaves of transgenic tobacco. Planta 195:88-97.Google Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Hillar Eichelmann
    • 1
  • Eero Talts
    • 1
  • Vello Oja
    • 1
  • Bahtijor Rasulov
    • 1
  • Evi Padu
    • 1
  • Agu Laisk
    • 1
  1. 1.Tartu Ülikooli Molekulaar- ja Rakubioloogia InstituutTartuEstonia

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