Arrangement of Chlorophyll Protein Complexes Determines Chloroplast Structure

  • Rumak Izabela
  • Gieczewska Katarzyna
  • Koziol-Lipinska Joanna
  • Kierdaszuk Borys
  • Mostowska Agnieszka
  • Garstka Maciej
Conference paper

Abstract

It is believed that there is a close relation between chloroplast structure and function. To support this statement we studied chloroplast structure and function in two plant species with a different susceptibility for chilling — bean (Phaseolus vulgaris) — CS (chilling sensitive) plant and pea (Pisum sativum) — CT (chilling tolerant) plant. The fluorescence difference spectrum bean — minus — pea indicated that there is relatively less core PSII and external antenna complexes associated with PSII and PSI in bean than in pea thylakoids. Instead the appearance of the emission band at around 705 nm suggested that the aggregated form of LHCII is more frequent in bean thylakoid membranes than in pea ones. Bigger appressed domains (grana) occurred in pea as compared to bean revealed by 3D structure of chloroplasts obtained using confocal laser scanning fluorescence microscopy CLSM).

Keywords

Chloroplast structure Chl fluorescence CLSM pea bean 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Rumak Izabela
    • 1
    • 2
  • Gieczewska Katarzyna
    • 1
    • 2
  • Koziol-Lipinska Joanna
    • 2
  • Kierdaszuk Borys
    • 3
  • Mostowska Agnieszka
    • 2
  • Garstka Maciej
    • 1
  1. 1.Department of Metabolic RegulationUniversity of WarsawWarsawPoland
  2. 2.Department of Plant Anatomy and CytologyUniversity of WarsawWarsawPoland
  3. 3.Department of BiophysicsUniversity of WarsawWarsawPoland

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