Alteration of O-J-I-P Chlorophyll Induction Kinetics by Dichromate: An Effect on the Water-Splitting System

  • Radovan Popovic
  • François Perreault
  • David Dewez
  • Dionne Jérome
  • Philippe Juneau

Abstract

The rapid chlorophyll fluorescence transients related to Photosystem II (PSII) activity were investigated when cells of the alga Chlamydomonas reinhardtii were exposed to dichromate ions. We found that dichromate produced an inhibitory effect seen as quenching of variable fluorescence yield in all rapid fluorescence transients: O-J, J-I and I-P. The decreased fluorescence yield at the O-J transient for dichromate-treated algae in dark or light-adapted samples resulted from alteration of the D1 protein. Decreased activation energy for the O-J step in light-adapted samples treated with dichromate indicated a decrease in the number of active PSII reaction centers having a functional D1 protein. The low activation energy for the J-I step for light-adapted samples, regardless of the dichromate effect, was caused by a large fraction of QB having already been reduced by continuous illumination. The activation energy for the I-P step was negative for light-adapted samples under dichromate treatment, showing that most of the remaining, active PSII reaction centers were reduced under continuous illumination. However, it cannot be excluded that the change of activation energy for the O-J and I-P steps resulted from a deteriorating effect of dichromate on the PSII water splitting system.

Keywords

Dichromate O-J-I-P PSII electron transport activation energy 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Radovan Popovic
    • 1
  • François Perreault
    • 1
  • David Dewez
    • 2
  • Dionne Jérome
    • 1
  • Philippe Juneau
    • 3
  1. 1.Department of Chemistry-TOXENUniversity of Quebec in MontrealMontreal, QuebecCanada
  2. 2.Department of Plant and Microbial BiologyUniversity of California, BerkeleyBerkelyUSA
  3. 3.Department of Biological Sciences-TOXEN, Canadian Research Chair on Ecotoxicology of Aquatic MicroorganismsUniversity of Quebec in MontrealMontreal, QuebecCanada

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