Substrate Water Bound to the S2-State of the Mn4OxCa Cluster in Photosystem II Studied by Advanced Pulse EPR Spectroscopy

  • Ji-Hu Su
  • Wolfgang Lubitz
  • Johannes Messinger
Conference paper

Abstract

In The Present Study, We Employed Several Advanced Pulse Epr Techniques To Detect Weak Hyperfine Couplings To Study Substrate Water Binding To The Mn4OXCa Cluster In The S2, State Of Photosystem Ii. Our Data Show That The Hyperfine Coupling Between The 2H And The Mn4OXCa Cluster Is Too Weak To Be Resolved. We Propose That The Information Does Not Allow Make Precise Conclusions About The Mode Of Substrate Water Binding To The Mn4OXCa Cluster. Our Preliminary Data Suggest That More Detailed Information Can Be Obtained From The H2 16O/ H2 17O Exchange Experiments.

Keywords

Water binding PSII pulse EPR ESEEM HYSCORE Mims ENDOR 

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References

  1. Åhrling KA, Evans MCW, Nugent JHA, Ball RJ, Pace RJ (2006) ESEEM studies of substrate water and small alcohol binding to the oxygen-evolving com- plex of photosystem II during functional turnover. Biochemistry 45:7069-7082.PubMedCrossRefGoogle Scholar
  2. Britt RD, Peloquin JM, Campbell KA (2000) Pulsed and parallel-polarization EPR characterization of the photosystem II oxygen-evolving complex. Annu Rev Biophys Biomol Struct 29:463-495.PubMedCrossRefGoogle Scholar
  3. Fiege R, Zweygart W, Bittl R, Adir N, Renger G, Lubitz W (1996) EPR and ENDOR studies of the water oxidizing complex of Photosystem II. Photosynth Res 48:227-237.CrossRefGoogle Scholar
  4. Hillier W, Messinger J (2005) Mechanism of photosyn-thetic oxygen production. In: Wydrzynski T, Satoh K (eds) Photosystem II. The Light-Driven Water: Plastoquinone Oxidoredutase. Springer, Dordrecht, The Netherlands, pp 567-608.CrossRefGoogle Scholar
  5. Kulik LV, Epel B, Lubitz W, Messinger J (2005) 55Mn pulse ENDOR at 34 GHz of the S0 and S2 states of the oxy-gen-evolving complex in photosystem II. J Am Chem Soc 127:2392-2393.PubMedCrossRefGoogle Scholar
  6. McEvoy JP, Brudvig GW (2006) Water-splitting chemistry of photosystem II. Chem Rev 106:4455-4483.PubMedCrossRefGoogle Scholar
  7. Su JH, Lubitz W, Messinger J (2008) Probing Mode and Site of Substrate Water Binding to the Oxygen-Evolving Complex in the S2 State of Photosystem II by 17O-HYSCORE Spectroscopy. J Am Chem Soc (in press)Google Scholar
  8. Tan XL, Gultneh Y, Sarneski JE, Scholes CP (1991) EPR-ENDOR of the electronic structure from two nitrog-enously ligated bis(µ-Oxo)-MnIII-MnIV model complexes spectroscopically relevant to the multi-manganese venter of photosystem-II. J Am Chem Soc 113:7853-7858.CrossRefGoogle Scholar
  9. Turconi S, MacLachlan DJ, Bratt PJ, Nugent JHA, Evans MCW (1997) Analysis of the interaction of water with the manganese cluster of photosystem II using isotopically labeled water. Biochemistry 36:879-885.PubMedCrossRefGoogle Scholar
  10. Yamada H, Mino H, Itoh S (2007) Protons bound to the Mn cluster in photosystem II oxygen evolving complex detected by proton matrix ENDOR. Biochim Biophys Acta 1767:197-203.PubMedCrossRefGoogle Scholar
  11. Yano J, Kern J, Sauer K, Latimer MJ, Pushkar Y, Biesiadka J, Loll B, Saenger W, Messinger J, Zouni A, Yachandra VK (2006) Where water is oxidized to dioxygen: Structure of the photosynthetic Mn4Ca cluster. Science 314:821-825.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Ji-Hu Su
    • 1
  • Wolfgang Lubitz
    • 1
  • Johannes Messinger
    • 1
  1. 1.Max-Planck-Institut für Bioanorganische ChemieMülheim an der RuhrGermany

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