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Role of Phosphatidylglycerol in Oxygen-Evolving Complex of Photosystem II

  • Naoki Mizusawa
  • Isamu Sakurai
  • Hisako Kubota
  • Hajime Wada
Conference paper

Abstract

Properties Of Photosystem Ii (Psii) In The Pgsa Mutant Of Synechocystis Sp. Pcc6803, Which Is Defective For The Biosynthesis Of Phosphatidylglycerol (Pg), Were Analyzed To Clarify The Role Of Pg In Oxygen-Evolving-Complex (Oec) Of Psii. Analyses Of Purified Psii Complexes Indicated That Psii From Pg-Depleted Pgsa Mutant Sustained Only 50% Of The Oxygen-Evolving Activity Compared To Wild-Type Cells And Dissociated The Extrinsic Proteins Of Psbo, Psbv And Psbu, And Mn Ions. The Released Psbo Re-Bound To Psii When Pg Was Added Back To The Pg-Depleted Mutant Cells. The Extent Of Inactivation Of Oxygenevolution In The Pg-Depleted Mutant Cells By Heat Treatment Or Dark Incubation Resembled Those Of ▵Psbo And ▵Psbv Mutant Cells. These Results Suggest That Pg Plays An Important Role In The Binding Of Extrinsic Proteins Required For Sustaining A Functional Mn-Cluster.

Keywords

Mn-cluster oxygen-evolving complex phosphatidylglycerol photosystem II Synechocystis sp. PCC6803 

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References

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Naoki Mizusawa
    • 1
  • Isamu Sakurai
    • 1
  • Hisako Kubota
    • 1
  • Hajime Wada
    • 1
  1. 1.Graduate School of Arts and SciencesUniversity of TokyoMeguro-ku, TokyoJapan

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