A Highly Efficient Protocol for Micropropagation of North American Ginseng

  • Sijun Zhou
  • Daniel C. W. Brown

North American ginseng (Panax quinquefolius L.) is a native herb of Canada and the United States and is cultivated for its highly valued root used for medicinal purposes. It has a long production cycle as seeds are usually produced after a 3-year cultivation and must stratify for additional 12–18 months before germination. A clonal propagation method based on in vitro procedures would contribute to its genetic improvement by reducing the generation cycle time, would allow for the reduction in confounding genotype effects in field evaluations through the use of clonal material and would be useful for germplasm preservation.

The tissue culture of Korean ginseng (Panax ginseng C.A. Meyer) is well studied with reports on callus cultures (e.g., Choi et al., 2003), protoplast culture (Arya et al., 1991), shoot organogenesis (e.g., Bonfill et al., 2002), somatic embryogenesis (e.g., Choi et al., 1999 and cited literature). These techniques have largely not been used successfully with American ginseng. There are only a Choi et al., 1999 and cited literature). These techniques have largely not been used successfully with American ginseng. There are only a few reports on the in vitro cultivation of North American ginseng. These cases report on the formation of somatic embryos on callus culture of embryonic, seedling, root or leaf explants (Brown et al., 2001; Zhou et al., 2006 and cited literature). North American ginseng is still considered to be recalcitrant in vitro because of the difficulty in obtaining plants with a well-developed root system.


Somatic Embryo Somatic Embryogenesis Panax Ginseng Cotyledon Explants Plant Cell Tissue Organ Cult 
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  1. Arya S, Liu JR, Eriksson T (1991) Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis. Plant Cell Rep 10: 277-281.CrossRefGoogle Scholar
  2. Bonfill M, Cusidó RM, Palazón J, Piñol MT, Morales C (2002) Influence of auxins on organogenesis and ginsenoside production in Panax ginseng cal-luses. Plant Cell Tissue Organ Cult 68: 73-78.CrossRefGoogle Scholar
  3. Brown DCW, Amyot L, Rintoul T (2001) Micropropagation of North American ginseng (Panax quinquefolius L.)—from test-tube to the field. In: Punja ZK (ed) Proceedings of the International Ginseng Workshop.Google Scholar
  4. Choi YE, Yang DC, Yoon ES, Choi KT (1999) High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos. Plant Cell Rep 18: 493-499.CrossRefGoogle Scholar
  5. Choi YE, Jeong JH, Shin CK (2003) Hormone-independent embryo- genic callus production from ginseng cotyledons using high concentrations of NH4NO3 and progress towards bioreactor production. Plant Cell Tissue Organ Cult 72: 229-235.CrossRefGoogle Scholar
  6. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50: 151-158.CrossRefPubMedGoogle Scholar
  7. Murashige T, Skoog F (1962) A reversed medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.CrossRefGoogle Scholar
  8. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199-204.CrossRefGoogle Scholar
  9. Zhou S, Brown DCW (2006) High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants. Plant Cell Rep 25: 166-173.CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Sijun Zhou
    • 1
  • Daniel C. W. Brown
    • 1
  1. 1.Southern Crop and Food Research Centre, Agriculture and Agri-Food CanadaLondonCanada

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