Advertisement

Transcriptome Profiling of Wheat Genotypes Differing in Bread Baking Quality under Different Environments

  • Yongfang Wan
  • Rowan Mitchell
  • Tim Wheeler
  • Mike Gooding
  • Rebecca Poole
  • Keith Edwards
  • Clare Mills
  • Peter Skeggs
  • Salvador Gezan
  • Sue Welham
  • Christina Shenton
  • Tong Zhu
  • Peter Shewry
Conference paper

Preliminary comparisons showed that the public Affymetrix array platform was more sensitive in detecting changes in gene expression in developing wheat grain than a cDNA array platform. Affymetrix arrays were therefore used to compare gene expression patterns in developing grain of seven doubled haploid lines (DHLs) from a cross between wheat cvs Spark ×Rialto, showing that over 440 genes were differentially expressed (significant at P <0.05). The seven DH lines also exhibited differences in processing properties and a number of the differentially expressed transcripts were associated with these.

Keywords

Doubled Haploid Doubled Haploid Line Array Platform Loaf Volume Affymetrix Array 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Gooding MJ, Ellist RH, Shewry PR, Schofield JD (2003) Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat. J Cereal Sci 37: 295-309.CrossRefGoogle Scholar
  2. Wilson ID, Barker GLA, Beswick RW, Shepherd SK, Lu CG, Coghill JA, Edwards D, Owen P, Lyons R, parker JS, Lenton JR, Holdsworth MJ, Shewry PR, Edwards KJ (2004) A transcriptomics resource for wheat functional genomics. Plant Biotechnol J 2: 495-506.CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Yongfang Wan
    • 1
  • Rowan Mitchell
    • 1
  • Tim Wheeler
    • 2
  • Mike Gooding
    • 2
  • Rebecca Poole
    • 3
  • Keith Edwards
    • 3
  • Clare Mills
    • 4
  • Peter Skeggs
    • 5
  • Salvador Gezan
    • 1
  • Sue Welham
    • 1
  • Christina Shenton
    • 6
  • Tong Zhu
    • 6
  • Peter Shewry
    • 1
  1. 1.Rothamsted ResearchUK
  2. 2.Reading UniversityUK
  3. 3.University of BristolUK
  4. 4.IFRUK
  5. 5.RHM Technology Ltd.UK
  6. 6.Syngenta Inc.USA

Personalised recommendations