Micropropagation of Coast Redwood (Sequoia Sempervirens)
Sequoia sempervirens (Lamb.) Endl., coast redwood, is a long-lived evergreen gymnosperm belonging to the family Taxodiaceae. This species is endemic to the coastal regions of California and Oregon, USA (Srinivasan & Friis, 1989; Ma et al., 2005). The fossil records of the genus Sequoia can be traced back to the Jurassic Period, in China (Endo, 1951). The tree is characterized by a thick, fibrous, and deeply furrowed bark along with a fire-resistant reddish-brown heartwood. Leaves of S. sempervirens are dimorphic, including linear and scale-like leaves. The linear leaves are spirally arranged or occasionally sub-opposite (Ma et al., 2005). The tree is highly valuable not only for ornamental purposes as trees can grow up to 110 m in length, but also for industrial purposes as it grows quite vigorously, rarely suffers from disease or insect attack, and it is resistant to strong winds and other poor climatic conditions. It is the high longevity and size of Sequoia trees that allow for its substantial biomass accumulation (Busing & Fujimori, 2005). In some stands, it exceeds 3500 metric tons/hectar. Thus, S. sempervirens can be used in the timber industry (plywood), paper industry, as well as pulp industry. It is well suited for short rotation coppicing.
KeywordsSomatic Embryo Somatic Embryogenesis Shoot Elongation Shoot Proliferation Stem Segment
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- Ahuja, M.R., Devey, M.E., Groover, A.T., Jermstad, K.D. & Neale, D.B. (2004) Mapped DNA probes from loblolly pine can be used for restriction fragment length polymorphism mapping in other conifers. Theor. Appl. Genet. 88, 279-282.Google Scholar
- Ball, E.A. (1987) Tissue culture multiplication of Sequoia. In: Bonga, J.M. & Durzan, D.L. (Eds) Cell and Tissue Culture in Forestry, vol 3. Martinus Nijhoff, Dordrecht, pp. 146-158.Google Scholar
- Bourgkard, F. & Favre, J.M. (1988) Somatic embryos from callus of Sequoia sempervirens. Plant Cell Rep. 7, 445-448.Google Scholar
- Fouret, Y., Larrieu, C. & Arnaud, Y. (1988) Rajeunissement in vitro chez la Sequoia sempervirens (Endl.). Ann Rech Sylv, AFOCEL, pp. 55-82.Google Scholar
- Hizume, M., Kondo, T., Shibata, F. & Ishizuka, R. (2001) Flow cytometric determination of genome size in the Taxodiaceae, Cupressaceae sensu stricto and Sciadopityaceae. Cytologia 66, 307-331.Google Scholar
- Srinivasan, V. & Friis, E.M. (1989) Taxodiaceous conifers from the Upper Cretaceous of Sweden. Biol. Skr. 35, 5-57.Google Scholar
- Staba, E.J. (1969) Plant tissue culture as a technique for the phytochemist. Recent Adv. Phytochem. 2, 75-102.Google Scholar
- Sul, I.-W. & Korban, S.S. (1994) Effect of different cytokinins on axillary shoot proliferation and elongation of several genotypes of Sequoia sempervirens. In Vitro Cell. Dev. Biol. 30P, 131-135.Google Scholar