The current status of single-particle X-ray imaging – or “X-ray crystallography without need for a crystal” – is reviewed, with particular emphasis on the Stony Brook/Cornell/Berkeley single yeast cell imaging project. Two-dimensional (2D) images of a quick-frozen yeast cell have been obtained with a resolution which, if carried out in three-dimensional (3D), would allow observation of the positions of the large molecular assemblies in an entire almost-living-state cell. The projected shift from 2D to 3D imaging is discussed.
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Sayre, D. (2007). Single-particle imaging. In: Read, R.J., Sussman, J.L. (eds) Evolving Methods for Macromolecular Crystallography. NATO Science Series, vol 245. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6316-9_15
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DOI: https://doi.org/10.1007/978-1-4020-6316-9_15
Publisher Name: Springer, Dordrecht
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