Low-temperature preservation of fish gonad cells and oocytes

  • Tiantian Zhang
  • David M. Rawson
  • Irena Pekarsky
  • Idit Blais
  • Esther Lubzens

Ensuring the retention of a record of the genetic diversity of species involves, at its simplest form, the long-term storage of material from somatic and reproductive cell lines. However, to have real practical benefit such material should be in the form of viable cellular material, ideally from the germline or reproductive cell lines. The ability to generate banks of cryopreserved sperm, eggs, and embryos that retain full viability following recovery from the frozen state would be the most powerful facilitating tool in species conservation and commercial applications such as aquaculture. The nuclear genomes of fish show a full range of sex chromosome differentiation, ranging from the all-autosomal karyotype as seen in the zebrafish to the genetically and cytogenetically differentiated sex chromosomes seen in the guppy (Traut and Winking, 2001). The preservation of haploid genomes in the viable form of male and female reproductive cells, or of viable embryos, is vital if normal populations of males and females are to be established.


Chum Salmon Primordial Germ Cell Danio Rerio Trypan Blue Staining Trypan Blue 
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Copyright information

© Springer 2007

Authors and Affiliations

  • Tiantian Zhang
    • 1
  • David M. Rawson
    • 1
  • Irena Pekarsky
    • 2
  • Idit Blais
    • 2
  • Esther Lubzens
    • 2
  1. 1.Luton Institute of Research in the Applied Natural SciencesUniversity of LutonLutonUK
  2. 2.National Institute of Oceanography, Israel Oceanographic and Limnological ResearchIsrael

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