Pseudomonas pp 227-251 | Cite as

Towards a Genome-Wide Mutant Library of Pseudomonas putida Strain KT2440

  • Estrella Duque
  • Antonio J. Molina-Henares
  • Jesús de la Torre
  • María A. Molina-Henares
  • Teresa del Castillo
  • Jennifer Lam
  • Juan L. Ramos

Microbiology is experiencing exciting times thanks to the current explosion of knowledge. About 25 years after Watson and Crick resolved the structure of DNA, Sanger’s and Maxam’s laboratories developed easy ways to determine the nucleotide sequence of a segment of DNA. This in turn led to the development of new technologies that now make it possible not only to decipher the complete genome sequence of an organism, but also to analyze the global patterns of expression of genes based on genomic microarrays or the results of proteomic assays. Nonetheless, although transcriptional arrays and proteomic techniques can identify large numbers of genes expressed under particular conditions, the biological meaning of these correlations is generally unclear without further analysis.

Keywords

Sludge Arsenate Proline Lysine Folic Acid 

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Copyright information

© Springer 2007

Authors and Affiliations

  • Estrella Duque
    • 1
  • Antonio J. Molina-Henares
    • 1
  • Jesús de la Torre
    • 1
  • María A. Molina-Henares
    • 1
  • Teresa del Castillo
    • 1
  • Jennifer Lam
    • 1
  • Juan L. Ramos
    • 1
  1. 1.Department of Environmental ProtectionEstación Experimental del Zaidín – CSICSpain

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