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Plant Molecular Farming: Host Systems, Technology and Products

  • Chapter
Applications of Plant Metabolic Engineering

Abstract

Plants have been used for various medicines by mankind over centuries and one quarter of prescription drugs are still of plant origin. The advances made in genomics and proteomics resulted in the isolation of several useful genes. These have been expressed in various expression systems including plants. The plant based production of pharmaceutically and industrially useful recombinant proteins is referred as molecular farming. Plants have been successfully genetically engineered using different methods. Stable nuclear transformation by Agrobacterium or biolistic methods, plastid engineering, use of viral based transient expression, agroinfiltration and magnifection techniques. In this review, an overview of plant molecular farming, host systems employed, vaccinogen production in plants with an emphasis on hepatitis B vaccine, technology perspective as well as the commercialized products are described

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References

  • Bakker H, Bardor M, Molthoff JW et al. (2001) Galactose-extended glycans of antibodies produced by transgenic plants, Proc Natl Acad Sci USA. 98: 2899–2904

    Article  PubMed  CAS  Google Scholar 

  • Bardor M, Faveeuw C, Fitchette AC et al. (2003a) Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha 1,3-fucose and core xylose, Glycobiology 13: 427–434

    Article  CAS  Google Scholar 

  • Bardor M, Loutelier-Bourhis C, Paccalet T et al. (2003b) Monoclonal C5-1 antibody produced in transgenic alfalfa plants exhibits a N-glycosylation that is suitable for glyco-engineering into human-compatible structures. Plant Biotechnol J 1: 451–462

    Article  CAS  Google Scholar 

  • Barta A, Sommergruber K, Thomson D et al. (1986) The expression of a nopaline synthase- human growth hormone chimeric gene in transformed tobacco and sunflower callus tissue. Plant Mol Biol 6: 347–357

    Article  CAS  Google Scholar 

  • Baur A, Kaufmann F, Rolli H et al. (2005a) A fast and flexible PEG-mediated transient expression system in plants for high level expression of secreted recombinant proteins. J Biotechnol 119: 332–342

    Article  CAS  Google Scholar 

  • Baur A, Reski R, Gorr G, (2005b) Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by co-expression of human serum albumin in the moss Physcomitrella patens. Plant Biotechnol J 3: 331–340

    Article  CAS  Google Scholar 

  • Bendich AJ (1987) Why do chloroplast and mitochondria contain so many copies of their genome?. BioEssays 6: 279–282

    Article  PubMed  CAS  Google Scholar 

  • Boothe JG, Parmenter DL, Saponja JA (1997) Molecular farming in plants: oilseeds as vehicles for the production of pharmaceutical proteins. Drug Develop Res 42: 172–181

    Article  CAS  Google Scholar 

  • Borisjuk NV, Borisjuk LG, Logendra S et al. (1999) Production of recombinant proteins in plant root exudates. Nature Biotech 17: 466–469

    Article  CAS  Google Scholar 

  • Canizares MC, Nicholson L, Lomonossoff GP (2005) Use of viral vectors for vaccine production in plants. Immunol Cell Biol 83: 263–270

    Article  PubMed  CAS  Google Scholar 

  • Chikwamba R, Cunnick J, Hathaway D et al. (2002) A functional antigen in a practical crop: LT-B producing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT). Transgenic Res 11: 479–493

    Article  PubMed  CAS  Google Scholar 

  • Clendennen SK, Lopez-Gomez R, Gomez-Lim M et al. (1998) The abundant 31-kilodalton banana pulp protein is homologous to class III acidic chitinases. Phytochemistry 47: 613–619

    Article  PubMed  CAS  Google Scholar 

  • Conneely OM, Headon DR, O’Malley BW et al. (2001) Production of recombinant lactoferrin and lactoferrin polypeptides using cDNA sequences in various organisms, US Patent 6, 228, 614, 2001

    Google Scholar 

  • Cramer CL, Boothe JG, Oishi KK (1999) Transgenic plants for therapeutic proteins: Linking up stream and down stream strategies, In: Hammond J, McGarvey P, Yusibov V, (ed) Current topics Microbiology and Immunology, Plant biotechnology : New products and applications. Springer-Verlag, Berlin, p 95–118

    Google Scholar 

  • Cramer CL, Weissenborn DL, (1997) HMG2 promoter expression system and post harvest production of gene products in plants and plant cell cultures. US patent No. 5,670, 349, 1997

    Google Scholar 

  • Cramer CL, Weissenborn DL, Oishi KK et al. (1996) High level production of enzymatically active human lysosomal proteins in transgenic plants. In: Owen MRL, Pen J, (ed) Transgenic plants : A production system for industrial and pharmaceutical protein. Wiley, Chichester, p 299–310

    Google Scholar 

  • Daniell H, Guda C, McPherson DT et al. (1996) Hyper expression of a synthetic protein-based polymer gene. Meth Mol Biol 63: 359–371

    Google Scholar 

  • Daniell H, Khan MS, Allison L (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci 7: 84–91

    Article  PubMed  CAS  Google Scholar 

  • Daniell H, Streatfield SJ, Wycoff K, (2001) Medical molecular farming : production of antibodies, biopharmaceuticals and edible vaccines in plants. TIPS 6: 219–226

    CAS  Google Scholar 

  • DeCosa B, Moar W, Lee SB et al. (2001) Hyper-expression of the Bt Cry-2 Aa2 operon in chloroplast leads to formation of insecticidal crystals. Nature Biotechnol 19: 71–74

    Article  CAS  Google Scholar 

  • Delaney DE (2002) Choice of crop species and development of transgenic product lines. In: Hood EE, Howard JA, (ed) Plants as factories for protein production. Kluwer, Dordrecht, p 139–158

    Google Scholar 

  • Delaney D, Jilka J, Barker D et al. (2002) Production of aprotinin in transgenic maize seeds for the pharmaceutical and cell culture markets. In: Vasil IK, (ed) Plant biotechnology 2002 and beyond. Kluwer, Dordrecht, p 393–394

    Google Scholar 

  • De Zoeten GA, Penwick JR, Horisberger MA et al. (1989) The expression, localization and effect of a human interferon in plants. Virology 172: 213–222

    Article  PubMed  Google Scholar 

  • Dieryck W, Pagnier J, Poyart C et al. (1997) Human hemoglobin from transgenic tobacco. Nature 386: 29–30

    Article  PubMed  CAS  Google Scholar 

  • Dogan B, Mason HS, Richter L et al. (2000) Process options in hepatitis B surface antigen extraction from transgenic potato. Biotechnol Prog 16: 435–441

    Article  PubMed  CAS  Google Scholar 

  • Douce G, Giuliani MM, Giannelli V et al. (1998) Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin from Escherichia coli. Vaccine 16: 1065–1073

    Article  PubMed  CAS  Google Scholar 

  • During K (1988) Wound-inducible expression and secretion of T4 lysozyme and monoclonal antibodies in Nicotiana tabacum. Ph.D Thesis. Mathematish-Naturwissenschaftlichen Fakultaet der Universitaet Zu Koeln

    Google Scholar 

  • Edelbaum O, Stein D, Holland N et al. (1992) Expression of active human interferon in transgenic plants. J Interferon Res 12: 449–453

    PubMed  CAS  Google Scholar 

  • Elliott S, Lorenzini T, Asher S et al. (2003) Enhancement of therapeutic protein in vivo activities through glycoengineering. Nature Biotechnol 21: 414–421

    Article  CAS  Google Scholar 

  • Evangelista RL, Kusnadi AR, Howard JA et al. (1998) Process and economic evaluation of the extraction and purification of recombinant β-glucuronidase from transgenic corn. Biotechnol Prog 14: 607–614

    Article  PubMed  CAS  Google Scholar 

  • Fiedler U, Conrad U (1995) High-level production and long term storage of engineered antibodies in transgenic tobacco seeds. Biotechnology 13: 1090–1093

    Article  PubMed  CAS  Google Scholar 

  • Fiedler U, Phillips J, Artsaenko O et al. (1997) Optimization of ScFv production in transgenic plants. Immunotechnology 3: 205–216

    Article  PubMed  CAS  Google Scholar 

  • Fischer R, Emans N (2000) Molecular farming of pharmaceutical proteins. Transgenic Res 9: 279–299

    Article  PubMed  CAS  Google Scholar 

  • Fischoff DA (1992) Field performance of insect resistant crops. J Cell Biochem Suppl 16F: 199

    Google Scholar 

  • Fraley RT, Rogers SG, Horsch RB et al. (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80: 4803–4807

    Article  PubMed  CAS  Google Scholar 

  • Franken E, Teuschel U, Hain R (1997) Recombinant proteins from transgenic plants. Curr Opin Biotechnol 8: 411–416

    Article  PubMed  CAS  Google Scholar 

  • Franklin SE, Mayfield SP (2004) Prospects for molecular farming in the green alga Chlamydomonas reinhardtii. Curr Opin Plant Biol 7: 159–165

    Article  PubMed  CAS  Google Scholar 

  • Freytag LC, Clements JD (2004) Mucosal adjuvants. Vaccine 23: 1804–1813

    Article  CAS  Google Scholar 

  • Ganz PR, Dudani AK, Tackaberry ES et al. (1996) Expression of human blood proteins in transgenic plants: The cytokine GM-CSF as a model protein. In: Owen MRL, Pen J (ed) Transgenic plants: A production system for industrial and pharmaceutical proteins. John Wiley and Sons, London, UK, p281–297

    Google Scholar 

  • Gaume A, Komarnytsky S, Borisjuk N et al. (2003) Rhizosecretion of recombinant proteins from plant hairy roots. Plant Cell Rep 21: 1188–1193

    Article  PubMed  CAS  Google Scholar 

  • Gleba Y, Klimyuk V, Marillonnet S (2005) Magnifection – a new platform for expressing recombinant vaccines in plants. Vaccine 23: 2042–2048

    Article  PubMed  CAS  Google Scholar 

  • Gomord V, Chamberlain P, Jefferis R et al. (2005) Biopharmaceuticals production in plants : problems, solutions and opportunities. TIBTECH 23: 559–565

    CAS  Google Scholar 

  • Gomord V, Denmat LA, Fitchette-Laine AC et al. (1997) The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum(ER) but promotes vacuolar targeting of proteins that escape the ER. Plant J 11: 313–325

    Article  PubMed  CAS  Google Scholar 

  • Gomord V, Sourrouille C, Fitchette AC et al. (2004) Production and glycosylation of Plant-made pharmaceuticals: the antibodies as a challenge. Plant Biotechnol J 2: 83–100

    Article  PubMed  CAS  Google Scholar 

  • Gorr G, Jost W (2005) Glycosylation design in transgenic moss for better product efficacy. BioProcessing J 4:26–30

    Google Scholar 

  • Grevich JJ, Daniell H (2005) Chloroplast genetic engineering: Recent advances and future perspectives. Critical Rev Plant Sci 24: 83–107

    Article  CAS  Google Scholar 

  • Gruber V, Berna PP, Arnaud T et al. (2001) Large-scale production of a therapeutic protein in transgenic tobacco plants: effect of subcellular targeting on quality of a recombinant dog gastric lipase. Mol Breed 7: 329–340

    Article  CAS  Google Scholar 

  • Hamamoto H, Sugiyama Y, Nakagawa N et al. (1993) A new tobacco mosaic virus vector and its use for the systemic production of angiotensin –1 converting enzyme inhibitor in transgenic tobacco and tomato. Biotechnology 11: 930–932

    Article  PubMed  CAS  Google Scholar 

  • Haq TA, Mason HS, Clements JD et al. (1995) Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268: 714–716

    Article  PubMed  CAS  Google Scholar 

  • Herbers K, Wilke I, Sonnewald U (1995) A thermostable xylanase from Closteridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified. Biotechnology 13: 63–66

    Article  CAS  Google Scholar 

  • Hiatt A, Cafferkey R, Bowdish K (1989) Production of antibodies in transgenic plants. Nature 342: 76–78

    Article  PubMed  CAS  Google Scholar 

  • Higo K, Saito Y, Higo H, (1993) Expression of a chemically synthesized gene for human epidermal growth factor under the control of cauliflower mosaic virus 35S promoter in transgenic tobacco. Biosci Biotech Biochem 57: 1477–1481

    Article  CAS  Google Scholar 

  • Hood EE, Witcher DR, Maddock S et al. (1997) Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol Breed 3: 291–306

    Article  CAS  Google Scholar 

  • Hood EE, Woodard SL, Horn ME (2002) Monoclonal antibody manufacturing in transgenic plants–myths and realities. Curr Opin Biotechnol 13: 630–635

    Article  PubMed  CAS  Google Scholar 

  • Horn ME, Pappu KM, Bailey MR et al. (2004) Advantageous features of plant based systems for the development of HIV vaccines. J Drug Targeting 11: 539–545

    Article  CAS  Google Scholar 

  • Huang Z, Santi L, LePore K et al. (2006) Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice. Vaccine 24: 2506–2513

    Article  PubMed  CAS  Google Scholar 

  • Huether CM, Lienhart O, Baur A et al. (2005) Glyco-engineering of moss lacking plant-specific sugar residues. Plant Biol 7: 292–299

    Article  PubMed  CAS  Google Scholar 

  • Imani J, Berting A, Nitsche S et al. (2002) The integration of a major hepatitis B virus gene into cell-cycle synchronized carrot cell suspension cultures and its expression in regenerated carrot plants. Plant Cell Tissue Org Cult 71: 157–164

    Article  CAS  Google Scholar 

  • James EA, Wang C, Wang Z et al. (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Prot Expr Purif 19: 131–138

    Article  CAS  Google Scholar 

  • Jefferson RA, Kavanaugh TA, Bevan MW (1987) GUS fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907

    PubMed  CAS  Google Scholar 

  • Jilka J (1999) Have your vaccine and eat it too. In: Global Agbiotech progress Proceedings of the AgBiotech World Forum ‘99, Global AG BioTech Progress, Drug and Market Development Publications, p 89–95

    Google Scholar 

  • Jost W, Link S, Horstmann V et al. (2005) Isolation and characterization of three moss-derived beta-tubulin promoters suitable for recombinant expression. Curr Genet 47: 111–120

    Article  PubMed  CAS  Google Scholar 

  • Joung YH, Youm JW, Jeon JH (2004), Expression of the hepatitis B surface S and pre S2 antigens in tubers of Solanum tuberosum. Plant Cell Rep 22: 925–930

    Article  PubMed  CAS  Google Scholar 

  • Kang TJ, Han SC, Kim MY et al. (2004) Expression of non-toxic mutant of Escherichia coli heat-labile enterotoxin in tobacco chloroplasts. Protein Expr Purif 38: 123–128

    Article  PubMed  CAS  Google Scholar 

  • Kapila J, De Rycke R, van Montagu M et al. (1996) An Agrobacterium mediated transient expression system for intact leaves. Plant Sci 122: 101–108

    Article  Google Scholar 

  • Kapusta J, Modelska A, Figlerowicz M et al. (1999) A plant derived edible vaccine against hepatitis B virus. FASEB J 13: 1796–1799

    PubMed  CAS  Google Scholar 

  • Khoudi H, Laberge S, Ferullo JM et al. (1999) Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol. Bioeng 64: 135–143

    Article  PubMed  CAS  Google Scholar 

  • Ko K, Tekoah Y, Rudd PM et al. (2003) Function and glycosylation of plant-derived antiviral monoclonal antibody. Proc Natl Acad Sci USA 10: 8013–8018

    Article  CAS  Google Scholar 

  • Komarnytsky S, Borisjuk NV, Borisjuk LG et al. (2000) Production of recombinant proteins in tobacco guttation fluid. Plant Physiol 124: 927–933

    Article  PubMed  CAS  Google Scholar 

  • Kong Q, Richter L, Yang YF et al. (2001) Oral immunization with hepatitis B surface antigen expressed in transgenic plants. Proc Natl Acad Sci USA 98: 11539–11544

    Article  PubMed  CAS  Google Scholar 

  • Koprivova A, Altmann F, Gorr G et al. (2003) N-glycosylation in the moss Physcomitrella patens is organized similarly to higher plants. Plant Biol 5: 582–591

    Article  CAS  Google Scholar 

  • Kumagai MH, Turpen TH, Weinzettl N et al. (1993) Rapid, high level expression of biologically active alpha-trichosanthin in transfected plant by an RNA viral vector. Proc Natl Acad Sci USA 90: 427–430

    Article  PubMed  CAS  Google Scholar 

  • Kusnadi AR, Hood EE, Witcher DR et al. (1998) Production and purification of two recombinant proteins from transgenic corn. Biotech Prog 14: 149–155

    Article  CAS  Google Scholar 

  • Kusnadi AR, Niklov ZL, Howard JA (1997) Production of recombinant proteins in transgenic plants: Practical considerations. Biotech Bioeng 56: 473–484

    Article  CAS  Google Scholar 

  • Larrick JW, Yu L, Naftzger C et al. (2001) Production of secretory IgA antibodies in plants. Biomol Engg 18: 87–94

    Article  CAS  Google Scholar 

  • Lauterslager TGM, Florack DEA, van der Wal TJ et al. (2001) Oral immunization of naïve and primed animals with transgenic potato tubers expressing LT-B. Vaccine 19: 2749–2755

    Article  PubMed  CAS  Google Scholar 

  • Legrand D, Salmon V, Spoik G et al. (2003) Recombinant lactoferrin, methods of production from plants and uses. US Patent 6 569 831

    Google Scholar 

  • Magnuson NS, Linzmaier PM, Reeves R et al. (1998) Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Prot Exp Purif 13: 45–52

    Article  CAS  Google Scholar 

  • Masarik M, Kizek R, Kramer KJ et al. (2003) Application of avidin-biotin technology and adsorptive transfer tripping square-wave voltammetry for detection of DNA hybridization and avidin maize. Anal Chem 75: 2663–2669

    Article  PubMed  CAS  Google Scholar 

  • Mason HS, Haq TA, Clements JD et al. (1998) Edible vaccine protects mice against Escherichia coli heat-labile enterotoxine (LT): potatoes expressing a synthetic LT-B gene. Vaccine 16: 1336–1343

    Article  PubMed  CAS  Google Scholar 

  • Mason HS, Lam DMK, Arntzen CJ (1992) Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci USA 89: 11745–11749

    Article  PubMed  CAS  Google Scholar 

  • Matsumoto S, Ikura K, Ueda M et al. (1995) Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells. Pant Mol Biol 27: 1163–1172

    Article  CAS  Google Scholar 

  • Mitchell VS, Philipose MM, Sanford JP (ed) (1993) The children’s vaccine initiative: achieving the vision, National Academy Press. p23

    Google Scholar 

  • Mitra A, Zhang Z (1994) Expression of a human lactoferrin cDNA in tobacco cells produces antibacterial protein(s). Plant Physiol 106: 997–981.

    Article  Google Scholar 

  • Molowa D, Schenouda M, Meyers A et al. (2002) The state of biologics manufacturing part II J.P. Morgan Market analysis of the state of manufacturing of biologics including mAbs. p 1–16

    Google Scholar 

  • Muntz K (1998) Deposition of storage proteins. Deposition of storage proteins, Plant Mol Biol 38: 77–99

    Google Scholar 

  • Nandi S, Suzuki YA, Huang J et al. (2002) Expression of human lactoferrin in transgenic rice grains for the application in infant formula. Plant Sci 163: 713–722

    Article  CAS  Google Scholar 

  • Newman T, Ohme-Takagi M, Taylor C et al. (1993) DST sequences, highly conserved among plant SAUR genes, target reporter transcripts for rapid decay in tobacco. Plant Cell 5: 701–714

    Article  PubMed  CAS  Google Scholar 

  • Nikolov Z, Hammes D (2002) Production of recombinant proteins from transgenic crops, In: Hood EE, Howard J (ed) Plants as Factories for Protein Production. Kluwer Academic Publishers, Dordrecht, p 159–174

    Google Scholar 

  • Palacpac NQ, Yoshida S, Sakai H et al. (1999) Stable expression of human 1,4-galactosyltransferase in plant cells modifies N-linked glycosylation patterns. Proc Natl Acad Sci USA 96: 4692–4697

    Article  PubMed  CAS  Google Scholar 

  • Pen J, Sijmons PC, van Ooijen AJJ et al. (1993) Protein production in transgenic crops: analysis of plant molecular farming. In: Hiatt A (ed) Transgenic plants fundamentals and applications. Marcell Dekker, NY, p 239–254

    Google Scholar 

  • Peng J, Song Q, Chengkui T (2002) Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta). Chinese Sci Bullt 47: 1438–1440

    Article  Google Scholar 

  • Peumans WJ, Proost P, Swennen RL et al. (2002) The abundant class III chitinase homolog in young developing banana fruits behaves as a transient vegetative storage protein and most probably serves as an important supply of aminoacids for the synthesis of ripening-associated proteins. Plant Physiol 130: 1063–1072

    Article  PubMed  CAS  Google Scholar 

  • Rappuoli R, Pizza M, Douce G et al. (1999) Structure and mucosal adjuvanticity of cholera and Escherichia coli heat-labile enterotoxins. Immunol Today 20: 493–500

    Article  PubMed  CAS  Google Scholar 

  • Richter L, Kipp PB (1999) Transgenic plants as edible vaccines. In: Hammond J, McGarvey P, Yusibov V (ed) Plant biotechnology-new products and applications. Springer-Verlag, New York, p159–176

    Google Scholar 

  • Richter LJ, Thanavala Y, Arntzen CJ et al. (2000) Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nature Biotechnol 18: 1167–1171

    Article  CAS  Google Scholar 

  • Ruggiero F, Exposito JY, Bournat P et al. (2000) Triple helix assembly and processing of human collagen produced in transgenic tobacco plants. FEBS Lett 469: 132–136

    Article  PubMed  CAS  Google Scholar 

  • Rymerson RT, Menassa R, Brandle JE (2002) Tobacco, a platform for the production of recombinant proteins. In: Erickson L, Yu WJ, Brandle J, Rymerson R (ed) Molecular farming of plants and animals for human and veterinary medicine. Kluwer Academic Publishers, Netherlands, p1–31

    Google Scholar 

  • Salmon V, Legrand D, Slomianny MC et al. (1998) Production of human lactoferrin in transgenic tobacco plants. Prot Expr Purif 13: 127–135

    Article  CAS  Google Scholar 

  • Samyn-Petit B, Gruber V, Flahaut C et al. (2001) N-Glycosylation potential of maize: the human lactoferrin used as a model. Glycoconjugate J 18: 519–527

    Article  CAS  Google Scholar 

  • Sandhu JS, Osadjan MD, Krasnyanski SF et al. (1999) Enhanced expression of the human respiratory syncytial virus: F gene in apple leaf protoplast. Plant Cell Rep 18: 394–397

    Article  CAS  Google Scholar 

  • Santi L, Giritch A, Roy CJ et al. (2006) Protection conferred by recombinant Yersinia pestis antigen produced by a rapid and highly scalable plant expression system. Proc Natl Acad Sci USA 103: 861–866

    Article  PubMed  CAS  Google Scholar 

  • Schouten A, Roosien J, Van Engelen FA et al. (1996) The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol Biol 30: 781–793

    Article  PubMed  CAS  Google Scholar 

  • Shanks JV, Morgan J (1999) Plant ‘hairy root’ culture. Curr Opin Biotechnol 10: 151–155

    Article  PubMed  CAS  Google Scholar 

  • Sijmons PC, Dekker BMM, Schrammeijer B et al. (1990) Production of correctly processed human serum albumin in transgenic plants. Biotechnology 8: 217–221

    Article  PubMed  CAS  Google Scholar 

  • Smirnov SP, Teveroskaia EKH, Krasheninnikova LV et al. (1990) Design of an expression integrative vector and its application for introducing the human recombinant -interferon gene into plants. Genetika 26: 2111–2121

    PubMed  CAS  Google Scholar 

  • Smith ML, Keegan ME, Mason HS et al. (2002a) Factors Important in the Extraction, Stability and in vitro assembly of the hepatitis B surface antigen derived from recombinant plant systems. Biotechnol Prog 18: 538–550

    Article  CAS  Google Scholar 

  • Smith ML, Mason HS, Schuler ML (2002b) Hepatitis B surface antigen (HBsAg) expression in plant cell culture: kinetics of antigen accumulation in batch culture and its intracellular form. Biotech Bioeng 80: 812–822

    Article  CAS  Google Scholar 

  • Smith ML, Richter L, Arntzen CJ et al. (2003) Structural characterization of plant derived hepatitis B surface antigen employed in oral immunization studies. Vaccine 21: 4011–4021

    Article  PubMed  CAS  Google Scholar 

  • Sojikul P, Buehner N, Mason HS (2003) A plant signal peptide- hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells. Proc Nat Acd Sci USA 100: 2209–2214

    Article  CAS  Google Scholar 

  • Sriraman R, Bardor M, Sack M et al. (2004) Recombinant anti-hCG antibodies retained in the endoplasmic reticulum of transformed plants lack core-xylose and core- α(1,3)-fucose residues. Plant Biotechnol J 2: 279–288

    Article  PubMed  CAS  Google Scholar 

  • Staub JM, Garcia B, Graves J et al. (2000) High yield production of a human therapeutic protein in tobacco chloroplasts. Nat Biotechnol 18: 333–338

    Article  PubMed  CAS  Google Scholar 

  • Stevens LH, Stoopen GM, Elbers IJ et al. (2000) Effect of climate conditions and plant developmental stage on the stability of antibodies expressed in transgenic tobacco. Plant Physiol 124: 173–182

    Article  PubMed  CAS  Google Scholar 

  • Stoger E, Ma JK, Fischer R et al. (2005) Sowing the seeds of success: pharamaceutical proteins from plants. Curr Opin Biotechnol 16: 1–7

    Article  CAS  Google Scholar 

  • Streatfeld SJ, Meyer JM, Barker DK et al. (2002) Development of an edible subunit vaccine in corn against enterotoxigenic strains of Escherichia coli. In Vitro Cell Dev Biol Plant 38: 11–17

    Article  CAS  Google Scholar 

  • Streatfield SJ (2005) Oral hepatitis B vaccine candidates produced and delivered in plant material. Immunol Cell Biol 83: 257–262

    Article  PubMed  CAS  Google Scholar 

  • Sunil Kumar GB, Ganapathi TR, Bapat VA, (2004) Edible vaccines : Current Status and Future Prospects. Physiol Mol Biol Plants 10: 37–47

    Google Scholar 

  • Sunil Kumar GB, Ganapathi TR, Revathi CJ et al. (2003) Expression of hepatitis B surface antigen in tobacco cell suspension cultures. Prot. Expr. Purif. 32: 10–17

    Article  CAS  Google Scholar 

  • Sunil Kumar GB, Ganapathi TR, Revathi CJ et al. (2005b) Expression of hepatitis B surface antigen in transgenic banana plants. Planta 222: 484–493

    Article  CAS  Google Scholar 

  • Sunil Kumar GB, Ganapathi TR, Srinivas L et al. (2005a) Secretion of hepatitis B surface antigen in transformed tobacco cell suspension cultures. Biotechnol Lett 27: 927–932

    Article  CAS  Google Scholar 

  • Sunil Kumar GB, Ganapathi TR, Srinivas L et al. (2006b) Expression of hepatitis B surface antigen in potato hairy roots. Plant Sci 170: 918–925

    Article  CAS  Google Scholar 

  • Sunil Kumar GB, Srinivas L, Ganapathi TR et al. (2006a) Hepatitis B surface antigen expression in transgenic tobacco (Nicotiana tabacum) plants using four different expression cassettes. Plant Cell Tissue Org Cult 84: 315–323

    Article  CAS  Google Scholar 

  • Sutton DW, Havstad PK, Kemp JD (1992) Synthetic cryIIIA gene from Bacillus thuringiensis improved for high expression in plants. Transgenic Res 1: 228–236

    Article  PubMed  CAS  Google Scholar 

  • Tackaberry ES, Dudani AK, Prior F et al. (1999) Development of biopharmaceuticals in plant expression systems: Cloning, expression and immunological reactivity of cytomegalovirus glycoprotein B (UL55) in seeds of transgenic tobacco. Vaccine 17: 3020–3029

    Article  PubMed  CAS  Google Scholar 

  • Takase, K, Hagiwara K (1998) Expression of human alpha-lactalbumin in transgenic tobacco. J Biochem 123: 440–444

    PubMed  CAS  Google Scholar 

  • Taylor C, Green P (1995) Identification and characterization of genes with unstable transcripts (GUTS) in tobacco. Plant Mol Biol 28: 27–38

    Article  PubMed  CAS  Google Scholar 

  • Thanavala Y, Mahoney M, Pal S et al. (2005) Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci USA 102: 3378–3382

    Article  PubMed  CAS  Google Scholar 

  • Thanavala Y, Yang YF, Lyons P et al. (1995) Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc Natl Acad Sci USA 92: 3358–3361

    Article  PubMed  CAS  Google Scholar 

  • Twyman RM, Schillberg S, Fischer R (2005) Transgenic plants in the biopharmaceutical market. Expert Opin Emerging Drugs 10: 185–218

    Article  CAS  Google Scholar 

  • Twyman RM, Stoger E, Schillberg S et al. (2003) Molecular farming in plants : host systems and expression technology. TIBTECH 21: 570–578

    CAS  Google Scholar 

  • Van den Akker F, Pizza M, Rappuoli R et al. (1997) Crystal structure of a non-toxic mutant of heat labile enterotoxin, which is a potent mucosal adjuvant. Protein Sci 6: 2650–2654

    Article  PubMed  Google Scholar 

  • van Rooijen GJH, Moloney MM (1995) Structural requirements of oleosin domains for the subcellular targeting to the oil body. Plant Physiol 109: 1353–1361

    Article  PubMed  Google Scholar 

  • Vandekerckhove J, Damme VJ, van Lijsebettens M et al. (1989) Enkephalins produced in transgenic plants using modified 2S seed storage proteins. Biotechnology 7: 929–932

    Article  CAS  Google Scholar 

  • Vaquero C, Sack M, Chandler J et al. (1999) Transient expression of a tumor specific single chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci USA 96: 11128–11133

    Article  PubMed  CAS  Google Scholar 

  • von Schaewen A, Sturm A, O’Neill J et al. (1993) Isolation of a mutant Arabidopsis plant that lacks N-acetylglucosaminyltransferase 1 is unable to synthesize golgi modified complex N-linked glycans. Plant Physiol 102: 1109–1118

    Article  Google Scholar 

  • Wenderoth I, von Schaewen A (2000) Isolation and characterization of plant N-acetyl glucosaminyltransferase I (GntI) cDNA sequences. Functional analyses in the Arabidopsis cgl mutant and in antisense plants. Plant Physiol 123: 1097–1108

    Article  PubMed  CAS  Google Scholar 

  • Watson J, Koya V, Leppla SH et al. (2004) Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop. Vaccine 22: 4374–4384

    Article  PubMed  CAS  Google Scholar 

  • WHO/Unicef/The World Bank. State of the world’s vaccines and immunization 2002. Geneva: World Health Organization Department of Vaccines and Biologicals, 2002.

    Google Scholar 

  • Witcher DR, Hood EE, Petersen D et al. (1998) Commercial production of β-glucuronidase (GUS): a model system for the production of proteins in plants. Mol Breed 4: 301–312

    Article  CAS  Google Scholar 

  • Woodard SL, Mayor JM, Bailey MR et al. (2003) Maize-derived bovine trypsin: characterization of the first large-scale commercial protein product from transgenic plants. Biotechnol Appl Biochem 38: 123–130

    Article  PubMed  CAS  Google Scholar 

  • Woodleif WG, Chaplin JF, Campbell CR et al. (1981) Effect of variety and harvest treatments on protein yield of close grown tobacco. Tob Sci 25: 83–86

    Google Scholar 

  • Wright A, Morrison SL (1998) Effect of C2-associated carbohydrate structure on Ig effector function: studies with chimeric mouse-human IgG1 antibodies in glycosylation mutants of Chinese hamster ovary cells. J Immunol 160: 3393–3402

    PubMed  CAS  Google Scholar 

  • Zeitlin L, Olmsted SS, Moench TR et al. (1998) A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nat Biotechnol 16: 1361–1364

    Article  PubMed  CAS  Google Scholar 

  • Zhong GY, Peterson D, Delaney DE et al. (1999) Commercial production of aprotinin in transgenic maize seeds Mol Breed 5: 345–356

    Google Scholar 

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Authors and Affiliations

  1. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India

    G.B. Sunil Kumar, T.R. Ganapathi, L. Srinivas & V.A. Bapat

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  1. G.B. Sunil Kumar
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  2. T.R. Ganapathi
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  3. L. Srinivas
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  4. V.A. Bapat
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Editors and Affiliations

  1. Leiden University, The Netherlands

    R. Verpoorte

  2. Heinrich-Heine-Universität, Düsseldorf, Germany

    A.W. Alfermann

  3. Reliance Life Sciences Pvt. Ltd, Navi Mumbai, India

    T.S. Johnson

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Kumar, G.S., Ganapathi, T., Srinivas, L., Bapat, V. (2007). Plant Molecular Farming: Host Systems, Technology and Products. In: Verpoorte, R., Alfermann, A., Johnson, T. (eds) Applications of Plant Metabolic Engineering. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6031-1_2

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