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Human Cell Culture pp 41–52Cite as

Genetic Modification of Human Embryonic Stem Cells

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References

  • Albertini, R.J. (2001) HPRT mutations in humans: biomarkers for mechanistic studies. Mutat. Res., 489: 1–16.

    Article  PubMed  CAS  Google Scholar 

  • Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and Thomson, J.A. (2000) Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol., 227: 271–278.

    Article  PubMed  CAS  Google Scholar 

  • Aubert, J., Dunstan, H., Chambers, I., and Smith, A. (2002) Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation. Nat. Biotechnol., 20: 1240–1245.

    Article  PubMed  CAS  Google Scholar 

  • Baum, C., Forster, P., Hegewisch-Becker, S., and Harbers, K. (1994) An optimized electroporation protocol applicable to a wide range of cell lines. Biotechniques, 17: 1058–1062.

    PubMed  CAS  Google Scholar 

  • Billy, E., Brondani, V., Zhang, H., Muller, U., and Filipowicz, W. (2001) Specific interference with gene expression induced by long, double-stranded RNA in mouse embryonal teratocarcinoma cell lines. Proc. Natl. Acad. Sci. USA, 98: 14428–14433.

    Article  PubMed  CAS  Google Scholar 

  • Bronson, S.K. and Smithies, O. (1994) Altering mice by homologous recombination using embryonic stem cells. J. Biol. Chem., 269: 27155–27158.

    PubMed  CAS  Google Scholar 

  • Buzzard, J.J., Gough, N.M., Crook, J.M., and Colman, A. (2004) Karyotype of human ES cells during extended culture. Nat. Biotechnol., 22: 381–382; author reply 382.

    Article  PubMed  CAS  Google Scholar 

  • Cowan, C.A., Klimanskaya, I., McMahon, J., Atienza, J., Witmyer, J., Zucker, J.P., Wang, S., Morton, C.C., McMahon, A.P., Powers, D., et al. (2004) Derivation of embryonic stem-cell lines from human blastocysts. N. Engl. J. Med., 350: 1353–1356.

    Article  PubMed  CAS  Google Scholar 

  • Deng, C. and Capecchi, M.R. (1992) Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus. Mol. Cell Biol., 12: 3365–3371.

    PubMed  CAS  Google Scholar 

  • Deng, C., Thomas, K.R., and Capecchi, M.R. (1993) Location of crossovers during gene targeting with insertion and replacement vectors. Mol. Cell Biol., 13: 2134–2140.

    PubMed  CAS  Google Scholar 

  • Doetschman, T., Gregg, R.G., Maeda, N., Hooper, M.L., Melton, D.W., Thompson, S., and Smithies, O. (1987) Targeted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature, 330: 576–578.

    Article  PubMed  CAS  Google Scholar 

  • Draper, J.S., Smith, K., Gokhale, P., Moore, H.D., Maltby, E., Johnson, J., Meisner, L., Zwaka, T.P., Thomson, J.A., and Andrews, P.W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol., 22: 53–54.

    Article  PubMed  CAS  Google Scholar 

  • Eiges, R., Schuldiner, M., Drukker, M., Yanuka, O., Itskovitz-Eldor, J., and Benvenisty, N. (2001) Establishment of human embryonic stem cell-transfected clones carrying a marker for undifferentiated cells. Curr. Biol., 11: 514–518.

    Article  PubMed  CAS  Google Scholar 

  • Elbashir, S.M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 411: 494–498.

    Article  PubMed  CAS  Google Scholar 

  • Evans, M.J. and Kaufman, M.H. (1981) Establishment in culture of pluripotential cells from mouse embryos. Nature, 292: 154–156.

    Article  PubMed  CAS  Google Scholar 

  • Fehling, H.J., Swat, W., Laplace, C., Kuhn, R., Rajewsky, K., Muller, U., and von Boehmer, H. (1994) MHC class I expression in mice lacking the proteasome subunit LMP-7. Science, 265: 1234–1237.

    Article  PubMed  CAS  Google Scholar 

  • Finger, S., Heavens, R.P., Sirinathsinghji, D.J., Kuehn, M.R., and Dunnett, S.B. (1988) Behavioral and neurochemical evaluation of a transgenic mouse model of Lesch–Nyhan syndrome. J. Neurol. Sci., 86: 203–213.

    Article  PubMed  CAS  Google Scholar 

  • Fire, A., Xu, S., Montgomery, M.K., Kostas, S.A., Driver, S.E., and Mello, C.C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391: 806–811.

    Article  PubMed  CAS  Google Scholar 

  • Gropp, M., Itsykson, P., Singer, O., Ben-Hur, T., Reinhartz, E., Galun, E., and Reubinoff, B.E. (2003) Stable genetic modification of human embryonic stem cells by lentiviral vectors. Mol. Ther., 7: 281–287.

    Article  PubMed  CAS  Google Scholar 

  • Haavik, J. and Toska, K. (1998) Tyrosine hydroxylase and Parkinson's disease. Mol. Neurobiol., 16: 285–309.

    Article  PubMed  CAS  Google Scholar 

  • Hasty, P., Rivera-Perez, J., and Bradley, A. (1991) The length of homology required for gene targeting in embryonic stem cells. Mol. Cell Biol., 11: 5586–5591.

    PubMed  CAS  Google Scholar 

  • Jang, S.K. and Wimmer, E. (1990) Cap-independent translation of encephalomyocarditis virus RNA: structural elements of the internal ribosomal entry site and involvement of a cellular 57-kD RNA-binding protein. Genes Dev., 4: 1560–1572.

    Article  PubMed  CAS  Google Scholar 

  • Keller, G. (2005) Embryonic stem cell differentiation: emergence of a new era in biology and medicine. Genes Dev., 19: 1129–1155.

    Article  PubMed  CAS  Google Scholar 

  • Kuehn, M.R., Bradley, A., Robertson, E.J., and Evans, M.J. (1987) A potential animal model for Lesch–Nyhan syndrome through introduction of HPRT mutations into mice. Nature, 326: 295–298.

    Article  PubMed  CAS  Google Scholar 

  • Lakshmipathy, U., Pelacho, B., Sudo, K., Linehan, J.L., Coucouvanis, E., Kaufman, D.S., and Verfaillie, C.M. (2004) Efficient transfection of embryonic and adult stem cells. Stem Cells, 22: 531–543.

    Article  PubMed  Google Scholar 

  • London, B. (2001) Cardiac arrhythmias: from (transgenic) mice to men. J. Cardiovasc. Electrophysiol., 12: 1089–1091.

    Article  PubMed  CAS  Google Scholar 

  • Ludwig, T.E., Levenstein, M.E., Jones, J.M., Berggren, W.T., Mitchen, E.R., Frane, J.L., Crandall, L.J., Daigh, C.A., Conard, K.R., Piekarczyk, M.S., et al. (2006) Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol., 24: 185–187.

    Article  PubMed  CAS  Google Scholar 

  • Ma, Y., Ramezani, A., Lewis, R., Hawley, R.G., and Thomson, J.A. (2003) High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors. Stem Cells, 21: 111–117.

    Article  PubMed  CAS  Google Scholar 

  • Mansour, S.L., Thomas, K.R., and Capecchi, M.R. (1988) Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature, 336: 348–352.

    Article  PubMed  CAS  Google Scholar 

  • Martin, G.R. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. USA, 78: 7634–7638.

    Article  PubMed  CAS  Google Scholar 

  • Mitalipova, M.M., Rao, R.R., Hoyer, D.M., Johnson, J.A., Meisner, L.F., Jones, K.L., Dalton, S., and Stice, S.L. (2005) Preserving the genetic integrity of human embryonic stem cells. Nat. Biotechnol., 23: 19–20.

    Article  PubMed  CAS  Google Scholar 

  • Mountford, P., Zevnik, B., Duwel, A., Nichols, J., Li, M., Dani, C., Robertson, M., Chambers, I., and Smith, A. (1994) Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression. Proc. Natl. Acad. Sci. USA, 91: 4303–4307.

    Article  PubMed  CAS  Google Scholar 

  • Nairn, R.S., Adair, G.M., Porter, T., Pennington, S.L., Smith, D.G., Wilson, J.H., and Seidman, M.M. (1993) Targeting vector configuration and method of gene transfer influence targeted correction of the APRT gene in Chinese hamster ovary cells. Somat. Cell Mol. Genet., 19: 363–375.

    Article  PubMed  CAS  Google Scholar 

  • Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H., and Smith, A. (1998) Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell, 95: 379–391.

    Article  PubMed  CAS  Google Scholar 

  • Niwa, H., Burdon, T., Chambers, I., and Smith, A. (1998) Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev., 12: 2048–2060.

    Article  PubMed  CAS  Google Scholar 

  • Odorico, J.S., Kaufman, D.S., and Thomson, J.A. (2001) Multilineage differentiation from human embryonic stem cell lines. Stem Cells, 19: 193–204.

    Article  PubMed  CAS  Google Scholar 

  • Pedersen, R.A. (1986) Potency, lineage and allocation in preimplantation mouse embryos. In: Experimental Approaches to Mammalian Embryonic Development (Ed.: Rossant, J. and Pedersen, R.A.), Cambridge University Press, New York, pp. 3–33.

    Google Scholar 

  • Potter, H., Weir, L., and Leder, P. (1984) Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA, 81: 7161–7165.

    Article  PubMed  CAS  Google Scholar 

  • Robertson, E., Bradley, A., Kuehn, M., and Evans, M. (1986) Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature, 323: 445–448.

    Article  PubMed  CAS  Google Scholar 

  • Smithies, O. (1993) Animal models of human genetic diseases. Trends Genet., 9: 112–116.

    Article  PubMed  CAS  Google Scholar 

  • Smithies, O., Gregg, R.G., Boggs, S.S., Koralewski, M.A., and Kucherlapati, R.S. (1985) Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination. Nature, 317: 230–234.

    Article  PubMed  CAS  Google Scholar 

  • Stacey, A., Schnieke, A., McWhir, J., Cooper, J., Colman, A., and Melton, D.W. (1994) Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice. Mol. Cell Biol., 14: 1009–1016.

    PubMed  CAS  Google Scholar 

  • Takeda, J., Seino, S., and Bell, G.I. (1992) Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res., 20: 4613–4620.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, K.R. and Capecchi, M.R. (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell, 51: 503–512.

    Article  PubMed  CAS  Google Scholar 

  • Thomas, K.R., Deng, C., and Capecchi, M.R. (1992) High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. Mol. Cell Biol., 12: 2919–2923.

    PubMed  CAS  Google Scholar 

  • Thomas, K.R., Folger, K.R. and Capecchi, M.R. (1986) High frequency targeting of genes to specific sites in the mammalian genome. Cell, 44: 419–428.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., and Jones, J.M. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A., Kalishman, J., Golos, T.G., Durning, M., Harris, C.P., Becker, R.A., and Hearn, J.P. (1995) Isolation of a primate embryonic stem cell line. Proc. Natl. Acad. Sci. USA, 92: 7844–7848.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J.A., Kalishman, J., Golos, T.G., Durning, M., Harris, C.P., and Hearn, J.P. (1996) Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts. Biol. Reprod., 55: 254–259.

    Article  PubMed  CAS  Google Scholar 

  • Urbach, A., Schuldiner, M., and Benvenisty, N. (2004) Modeling for Lesch–Nyhan disease by gene targeting in human embryonic stem cells. Stem Cells, 22: 635–641.

    Article  PubMed  CAS  Google Scholar 

  • Vallier, L., Rugg-Gunn, P.J., Bouhon, I.A., Andersson, F.K., Sadler, A.J., and Pedersen, R.A. (2004) Enhancing and diminishing gene function in human embryonic stem cells. Stem Cells, 22: 2–11.

    Article  PubMed  CAS  Google Scholar 

  • Vasquez, K.M., Marburger, K., Intody, Z., and Wilson, J.H. (2001) Manipulating the mammalian genome by homologous recombination. Proc. Natl. Acad. Sci. USA, 98: 8403–8410.

    Article  PubMed  CAS  Google Scholar 

  • Weintraub, H., Cheng, P.F., and Conrad, K. (1986) Expression of transfected DNA depends on DNA topology. Cell, 46: 115–122.

    Article  PubMed  CAS  Google Scholar 

  • Wernig, M., Tucker, K.L., Gornik, V., Schneiders, A., Buschwald, R., Wiestler, O.D., Barde, Y.A., and Brustle, O. (2002) Tau EGFP embryonic stem cells: an efficient tool for neuronal lineage selection and transplantation. J. Neurosci. Res., 69: 918–924.

    Article  PubMed  CAS  Google Scholar 

  • Xian, H.Q., McNichols, E., St Clair, A., and Gottlieb, D.I. (2003) A subset of ES-cell-derived neural cells marked by gene targeting. Stem Cells, 21: 41–49.

    Article  PubMed  CAS  Google Scholar 

  • Xu, C., Inokuma, M.S., Denham, J., Golds, K., Kundu, P., Gold, J.D., and Carpenter, M.K. (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol., 19: 971–974.

    Article  PubMed  CAS  Google Scholar 

  • Yanez, R.J. and Porter, A.C. (1999) Influence of DNA delivery method on gene targeting frequencies in human cells. Somat. Cell Mol. Genet., 25: 27–31.

    Article  PubMed  CAS  Google Scholar 

  • Ying, Q.L., Stavridis, M., Griffiths, D., Li, M., and Smith, A. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol., 21: 183–186.

    Article  PubMed  CAS  Google Scholar 

  • Zaehres, H., Lensch, M.W., Daheron, L., Stewart, S.A., Itskovitz-Eldor, J., and Daley, G.Q. (2005) High-efficiency RNA interference in human embryonic stem cells. Stem Cells, 23: 299–305.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, G., Gurtu, V., and Kain, S.R. (1996) An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun., 227: 707–711.

    Article  PubMed  CAS  Google Scholar 

  • Zwaka, T.P. and Thomson, J.A. (2003) Homologous recombination in human embryonic stem cells. Nat. Biotechnol., 21: 319–321.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Baylor College of Medicine, Center for Cell and Gene Therapy and Department of Molecular and Cellular Biology, Houston, Texas, USA

    Thomas P. Zwaka

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  1. Thomas P. Zwaka
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Editors and Affiliations

  1. University College London, London, UK

    John R. Masters

  2. University of California, San Diego, CA, USA

    Bernhard O. Palsson

  3. University of Wisconsin Medical School, 470 N. Charter Street, 53706-1509, Madison, WI, USA

    James A. Thomson

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Zwaka, T.P. (2007). Genetic Modification of Human Embryonic Stem Cells. In: Masters, J.R., Palsson, B.O., Thomson, J.A. (eds) Human Cell Culture. Human Cell Culture, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5983-4_4

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