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Quality Control of Human Stem Cell Lines

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Human Cell Culture

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References

  • Andrews, P.A., Benvenisty, N., McKay, R., Pera, M.F., Rossant, J., Semb, H., and G.N., Stacey (2005) Commentary: The International Stem Cell Initiative: towards benchmarks for human embryonic stem cell research. Nat. Biotechnol., 23: 795–797.

    Article  PubMed  CAS  Google Scholar 

  • Andrews, P.W., Przyborski, S.A., and Thomson, J.A. (2001) Embryonal carcinoma cells as embryonic stem cells. In: Stem Cell Biology (Eds.: Marshak, D.R., Gardner, R.L., and Gottlieb, D.), Cold Spring Harbor Monograph Series, Cold Spring Harbor, New York, pp. 231–265.

    Google Scholar 

  • Baharvand, H., Hajheidari, M., Ashtiani, S.K., and Salekdeh, G.H. (2006) Proteomic signature of human embryonic stem cells. Proteomics, 6: 3544–9354.

    Article  PubMed  CAS  Google Scholar 

  • Beranger, F., Mange, A., Solassol, J., and Lehmann, S. (2001) Cell culture models of transmissible spongiform encephalopathies. Biochem. Biophys. Res. Commun., 289: 311–316.

    Article  PubMed  CAS  Google Scholar 

  • Bosque, P.J. and Prusiner, S.B. (2000) Cultured cell sublines highly susceptible to prion infection. J. Virol., 74: 4377–4386.

    Article  PubMed  CAS  Google Scholar 

  • Boyle, J.M., Hopkins, J., Fox, M., Allen, T.D., and Leach, R.H. (1981) Interference in hybrid clone selection caused by Mycoplasma hyorhinis infection. Exp. Cell Res., 132: 67–72.

    Article  PubMed  CAS  Google Scholar 

  • Buehring, G.C., Valesco, M., and Pan, C.Y. (1995) Cell culture contamination by mycobacteria. In Vitro Cell Dev. Biol. Anim., 31: 735–737.

    Article  PubMed  CAS  Google Scholar 

  • Chesebro, B., Wehrly, K., Caughey, B., et al. (1993) Foreign PrP expression and scrapie infection in tissue culture cell lines. Dev. Biol. Stand., 80: 131–140.

    PubMed  CAS  Google Scholar 

  • Christensen, B., Hansen, C., Debiek-Rychter, M., Kieler, J., Ottensen, S., and Schmidt, J. (1993) Identity of tumourigenic uroepithelial cell lines and ‘spontaneously’ transformed sublines. Br. J. Can., 68: 879–884.

    CAS  Google Scholar 

  • Coecke, S., Balls, M., Bowe, G., Davis, J., Gstraunthaler, G., Hartung, T., Hay, R., Merten, O.-W., Price, A., Shechtman, L., Stacey, G., and Stokes, W. (2005) Guidance on Good Cell Culture Practice: a report of the second ECVAM task force on good cell culture practice. ATLA, 33: 261–287.

    PubMed  CAS  Google Scholar 

  • Cobo, F., Stacey, G.N., Hunt, C., Cabrera, C., Nieto, A., Montes, R., Cortes, J.L., Catalina, P., Barnie, A., and Concha, A. (2005) Microbiological control in stem cell banks: approaches to standardisation. Appl. Microbiol. Biotechnol., 68: 456–466.

    Article  PubMed  CAS  Google Scholar 

  • Curry, P.T., Kropko, M.L., Garvin, J.R., Fiedler, R.D., and Thiess, J.C. (1996) In vitro induction of micronulclei and chromosome aberrations by quinolones: possible mechanisms. Mut. Res., 352: 143–150.

    Google Scholar 

  • Dang, S.M., Kyba, M., Perlingeiro, R., Daley, G.Q., and Zandstra, P.W. (2002) Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems. Biotechnol. Bioeng., 78: 442–453.

    Article  PubMed  CAS  Google Scholar 

  • Del Guidice, R.A. and Gardella, R.S. (1984) Mycoplasma infection of cell culture: effects, incidence and detection, In: In vitro Monograph 5, Uses and Standardisation of Vertebrate Cell Cultures, Tissue Culture Association, Gaithersberg, MD, USA, 1984, pp. 104–115.

    Google Scholar 

  • Denecke, J., Becker, K., Jurgens, H., Gross, R., and Wolff, J.E. (1999) Falsification of tetrazolium dye (MTT) based cytotoxicity assay results due to mycoplasma contamination of cell cultures. Anticancer Res., 19: 1245–1248.

    PubMed  CAS  Google Scholar 

  • Draper, J.S., Smith, K., Gokhale, P., Moore, H.D., Maltby, E., Johnson, J., Meisner, L., Zwaka, T.P., Thomson, J.A., and Andrews, P.W. (2004) Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol., 22: 53–54.

    Article  PubMed  CAS  Google Scholar 

  • Draper, J.S., Pigott, C., Thomson, J.A., and Andrews, P.A. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J. Anat., 200: 249–258.

    Article  PubMed  CAS  Google Scholar 

  • Doblhoff-Dier, O. and Stacey, G.N. (2006) Cell lines: applications and biosafety. In: Biological Safety: Principles and Practice, 4th Edn (Eds.: Flemmings, D.O. and Hunt, D.L.), ASM Press, Washington, DC, pp. 221–240.

    Google Scholar 

  • Drexler, H.G., Dirks, W.G., Matsuo, Y., MacLeod, R.A. (2003) False leukemia-lymphoma cell lines: an update on over 500 cell lines. Leukemia. 17: 416–426.

    Article  PubMed  CAS  Google Scholar 

  • Edwards, C., Belgrave, D., Janossy, G., Bradley, N.J., Stebbings, R., Gaines-Das, R., Thorpe, R., Sawle, A., Arroz, M.J., Brando, B., Gratama, J.W., Orfao de Matos, A., Papa, S., Papamichail, M., Lenkei, R., Rothe, G., and Barnett, D. (2005) Multicentre evaluation of stable reference whole blood for enumeration of lymphocyte subsets by flow cytometry. Cytometry B. Clin. Cytom., Jun 22; [Epub ahead of print].

    Google Scholar 

  • Erickson, G.A., Bolin, S.R., and Landgraf, J.D. (1991) Viral contamination of foetal bovin serum used for tissue culture: risks and concerns. Dev. Biol. Stand., 75: 173–175.

    PubMed  CAS  Google Scholar 

  • European Medicines Evaluation Agency (1997) Note for guidance on quality of biotechnological products: viral safety evaluation of biotechnology products derived from cell lines of human or animal origin (CPMP/ICH/295/95) European Medicines Evaluation Agency, 7 Westferry Circus, Canary Wharf, London, E14 4HB UK, (http://www.eudra.org/emea.html) .

  • European Pharmacopeia (2006a). European Pharmacopeia section 2.6.1 (Sterility), 5th Edition, Maisonneuve SA, Sainte Ruffine (www.pheur.org) .

  • European Pharmacopeia (2006b). European Pharmacopeia section 2.6.7 (Mycoplasma), 5th Edition, Maisonneuve SA, Sainte Ruffine.

    Google Scholar 

  • European Pharmacopoeia (2005) Minimising the risk of transmitting animal spongiform encephalopathy agents via human and veterinary medicinal products (5.2.8). European Pharmacopoeia 5.0, 01/2005:50208, pp. 463–471.

    Google Scholar 

  • European Union (2004) Directive 2004/23/EC of the European Parliament and the Council of 31 March 2004 on setting standards of quality and safety for the donation, processing, preservation, storage and distribution of human tissues and cells. Official Journal of the European Union, L102: 48–58.

    Google Scholar 

  • EU Commission of the European Communities (1998) The Rules Governing Medical Products in the European Community, Good Manufacturing Practice for Medicinal Products, Volume IV, April 1998, ISBN 9289–20297, with additional Annexes and update of legal references July 2004.

    Google Scholar 

  • Fabisiak, J.P., Weiss, R.D., Powell, G.A., and Dauber, J.H. (1993) Enhanced secretion of immune-modulating cytokines by human lung fibroblasts during in vitro infection with Mycoplasma fermentans. Am. J. Respir. Cell Mol. Biol., 8: 358–364.

    PubMed  CAS  Google Scholar 

  • Fleming, D.O. (2006) Indigenous and pathogenic agents of research animals. In: Biological Safety: Principles and Practices (Eds.: Fleming, D.O. and Hunt, D.L.), ASM Press, Washington, DC, pp. 19–34.

    Google Scholar 

  • Fok, E.Y. and Zandstra, P.W. (2005) Shear-controlled single-step mouse embryonic stem cell expansion and embryoid body-based differentiation. Stem Cells, 23: 1333–1342.

    Article  PubMed  CAS  Google Scholar 

  • Gartler, S.M. (1967) Genetic markers as tracers in cell culture. Natl. Cancer Inst. Monogr., 26: 167–195.

    PubMed  CAS  Google Scholar 

  • Gey, G.O., Coffman, W.O., and Kubiek, M.T. (1952) Tissue culture studies of proliferative capacity of cervicdl carcinoma and normal epithelium. Cancer Res., 12: 264.

    Google Scholar 

  • Gilbert, D.A., Reid, Y.A., Gail, M.H., Pee, D., White, C., Hay, R.J., and O'Brien, S.J. (1990) Application of DNA fingerprints for cell line individualisation. Am. J. Hum. Gen. 47: 499–517.

    CAS  Google Scholar 

  • Hallauer, C., Kronauer, G., and Siegl, G. (1971) Parvovirus contaminants of permanent human cell lines I virus isolation from 1960–1970. Arch. Gesamte Virusforsch., 35: 80–90.

    Article  PubMed  CAS  Google Scholar 

  • Hampe, J., Nurnberg, P., Epplen, C., Jahn, S., Grunow, R., and Epplen, J.T. (1992) Oligonucleotide fingerprinting as a means to identify and survey long term culture B cell hybridomas and T cell lines. Hum. Antibodies Hybridomas, 1992; 3: 186–190.

    PubMed  CAS  Google Scholar 

  • Harris, M. (1964) Cell Culture and Somatic Variation. Holt, Rinehart and Winston, USA.

    Google Scholar 

  • Healy, L., Hunt, C., Young, L., and Stacey, G. (2005) The UK Stem Cell Bank: its role as a public research resource centre providing access to well-characterised seed stocks of human stem cell lines. Adv. Drug Deliv. Rev., 57: 1981–1988.

    Article  PubMed  CAS  Google Scholar 

  • Hsu, T.C. (1952) Mammalian chromosomes in vitro the karyotype of man. J. Hered. 43: 167–172.

    Google Scholar 

  • Jeffreys, A.J., Wilson, V., and Thein, S.-L. (1985) Individual specific fingerprints of human DNA. Nature, 316: 76–79.

    Article  PubMed  CAS  Google Scholar 

  • Kita, M., Ohmoto, Y., Hirai, Y., Yamaguchi, N., and Imanishi, J. (1992) Induction of cytokines in human peripheral blood mononuclear cells by mycoplasmas. Microbiol. Immunol., 36: 507–516.

    PubMed  CAS  Google Scholar 

  • Kurosawa, H., Imamura, T., Koike, M., Sasaki, K., and Amano, Y. A simple method for forming embryoid body from mouse embryonic stem cells. J. Biosci. Bioeng., 96: 409–411.

    Google Scholar 

  • Lemaitre, M., Henin, Y., Destouesse, F., Ferrieux, C., Montagnier, L., Blanchard, A. (1992) Role of mycoplasma infection in the cytopathic effect induced by human immunodeficiency virus type 1 in infected cell lines. Infect. Immun., 60: 742–748.

    PubMed  CAS  Google Scholar 

  • Lipp, M., Koch, E., Brandner, G., Bredt, W. (1979) Simulation and prevention of retrovirus-specific reactions by mycoplasmas. Med. Microbiol. Immunol., 167(2): 127–136.

    Article  PubMed  CAS  Google Scholar 

  • Litt, M. and Luty, J.A. (1989) A hypervariable microsatellite revealed by in-vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Genet., 44: 397–401.

    CAS  Google Scholar 

  • Loring, J.F. and Rao, M.S. (2006) Establishing standards for the characterization of human embryonic stem cell lines. Stem Cells, 24: 145–150.

    Article  PubMed  Google Scholar 

  • MacLeod, R.A.F., Dirks, W.G., Matsuo, Y., Kaufman, M., Milch, H., and Drexler, H.G. (1999) Widespread intra-species cross-contamination of human tumour cell lines arising at source. Int. J. Cancer, 83: 555–563.

    Article  PubMed  CAS  Google Scholar 

  • Masters, J.R.W., Thompson, J., Daly-Burns, B., Reid, Y.A., Dirks, W.G., Packer, P.I., Toji, L.H., Ohno, T., Tanabe, T.H., Arlett, C.F., Kelland, L.R., Harrison, M., Virmani, A., Ward, T.H., Ayres, K.L., and Debenham, P.G. (2001) Short tandem repeat profiling provides international reference standards for human cell lines. PNAS (USA), 98: 8012–8017.

    Article  CAS  Google Scholar 

  • McGarrity, G.I., Kotani, H., and Butler, G.H. (1993) Mycoplasma and tissue culture cells. In: Mycoplasmas: Molecular Biology and Pathogenesis (Ed.: Maniloff, J.), ASM Press, pp. 445–454.

    Google Scholar 

  • McGarrity, G.I., Vanaman, V., and Sarama, J. (1984) Cytogenetic effects of infection of cell culture mycoplasmas: a review. In Vitro, 20: 1–18.

    Article  PubMed  CAS  Google Scholar 

  • Medicines Control Agency (2002) Rules and Guidance for Pharmaceutical Manufacturers and Distributors 2002. Her Majesty's Stationary Office, London.

    Google Scholar 

  • Melcher, R., Maisch, S., Koehler, S., Bauer, M., Steinlein, C., Schmid, M., Kudlich, T., Schauber, J., Luehrs, H., Menzel, T., and Scheppach, W. (2005) SKY and genetic fingerprinting reveal a cross-contamination of the putative normal colon epithelial cell line NCOL-1. Cancer Genet. Cytogenet., 158: 84–87.

    Article  PubMed  CAS  Google Scholar 

  • Milhavet, O., Casanova, D., Chevallier, N., McKay, R.D., and Lehmann, S. (2006) Neural stem cell model for prion propagation. Stem Cells, 24: 2284–2291.

    Article  PubMed  CAS  Google Scholar 

  • Minor, P.D., Will, R.G., and Salisbury, D. (2001) Vaccines and variant CJD. Vaccine 19: 3607.

    Article  Google Scholar 

  • Mowles, J.M., Doyle, A., Kearns, M.J., and Cerisier, Y. (1989) Isolation of an unusual fastidious contaminant bacterium from an adherent cell line using a novel technique. Advances in animal cell biology and technology for bioprocesses (Eds.: Spier, R.E., Griffiths, J.B., Stephenne, J., and Crooy, P.J.), Butterworths, Tiptree, pp. 111–114.

    Google Scholar 

  • Nelson-Rees, W.A., Daniels, D.W., and Flandermeyer, R.R. (1981) Cross-contamination of cells in culture. Science, 212: 446–452.

    Article  PubMed  CAS  Google Scholar 

  • Nicklas, W., Kraft, V., and Meyer, B. (1993) Contamination of transplantable tumour cell lines and monoclonal antibodies with rodent viruses. Lab. Anim. Sci., 43: 296–300.

    PubMed  CAS  Google Scholar 

  • O'Brien, S.J., Cliener, G., Olson, R. and Shannon, J.E. (1977) Enzyme polymorphisms as genetic signatures in human cell cultures. Science, 195: 1345–1348.

    Article  PubMed  Google Scholar 

  • Parson, W., Kirchebner, R., Muhlmann, R., Renner, K., Kofler, A., Schmidt, S., and Kofler, R. (2005) Cancer cell line identification by short tandem repeat profiling: power and limitations. FASEB J., 19: 434–436.

    PubMed  CAS  Google Scholar 

  • Pera, M., Reubinoff, B., and Thomson, J.A. (2000) Human embryonic stem cells. J. Cell Sci., 113: 5–10.

    PubMed  CAS  Google Scholar 

  • Peterson, W.D., Ottenbrit, M.J., and Hukku, B. (1984) Isoenzyme Analysis in Cell Culture Uses Standardisation of Vert. Cultures in vitro, Gaithesberg, USA.

    Google Scholar 

  • Paddenberg, R., Wulf, S., Heiman, P., Beck, L.A., and Mennherz, H.G. (1996) Internucleosomal DNA fragmentation in cultured cells under conditions reported to induce apoptosis may be caused by mycoplasma endonucleases. Eur. J. Cell Biol., 71: 105–119.

    PubMed  CAS  Google Scholar 

  • Polianskaia, G.G. and Efremova, T.N. (1993) The effect of mycoplasma contamination and decontamination with ciprofloxacin on the karyotypic structure of the Chinese hamster V-79 lung cell line. Tsitologiia, 35: 71–78.

    PubMed  CAS  Google Scholar 

  • Prowse, A.B., McQuade, L.R., Bryant, K.J., Van Dyk, D.D., Tuch, B.E., and Gray, P.P. (2005) A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells. Proteomics, 5: 978–989.

    Article  PubMed  CAS  Google Scholar 

  • Rottem, S. and Naot, Y. (1998) Subversion and exploitation of host cells by mycoplasma. Trends in Microbiol., 6: 436–440.

    Article  CAS  Google Scholar 

  • Rutzky, C.P., Kaye, C.J., Siciliano, M.J., Chao, M., and Kahan, B.D. (1980) Longitudinal karyotype and genetic signature analysis of cultures human colon adenocarcinoma cell lines LS180 and LS174T. Cancer Res., 40: 1443–1448.

    PubMed  CAS  Google Scholar 

  • Shimada, H. and Itoh, S. (1996) Effects of new quinolone antibacterial agents on mammalian chromosomes. J. Toxicol. Envir. Health, 47: 115–123.

    Article  CAS  Google Scholar 

  • Stacey, G.N. (2000) Detection of mycoplasma by DNA amplification. In: Cell and Tissue Culture for Medical Research (Eds.: Doyle, A. and Griffiths, J.B.), Wiley, Chichester, UK, pp. 58–61.

    Google Scholar 

  • Stacey, G.N. (2002) Standardisation of cell lines. Dev. Biologicals, 111: 259–272.

    CAS  Google Scholar 

  • Stacey, G. (2004) Validation of cell culture media components. Hum. Fertil., 7: 113–118.

    Article  Google Scholar 

  • Stacey, G.N., Parodi, B., and Doyle, A. (1995) The European Tissue Culture Society (ETCS) initiative on quality control of cell lines. J. Exp. Clin. Can. Res., 14: 210–211.

    Google Scholar 

  • Stacey, G.N., Bolton, B.J., Doyle, A., and Griffiths, J.B. (1992) DNA fingerprinting: a valuable new technique for the characterisation of animal cell lines. Cytotechnology, 9: 211–216.

    Article  PubMed  CAS  Google Scholar 

  • Stacey, G., Byrne, E., and Hawkins, R.J. (in press) DNA fingerprinting and characterisation of animal cell lines. In: Animal Cell Biotechnology: Methods and Protocols, 2nd Edition (Ed.: Joertner, R.), Hmana Press, Totowa, NJ, USA.

    Google Scholar 

  • Stacey, G.N., Cobo, F., Nieto, A., Talavera, P., Healy, L., and Concha, A. (2006) The development of ‘feeder’ cells for the preparation of clinical grade hES cell lines: challenges and solutions. J. Biotechnol., 125: 583–588.

    Article  PubMed  CAS  Google Scholar 

  • Stacey, G. and Doyle, A. (2000) Cell banks: a service to animal cell technology. In: Encyclopedia of Animal Cell Technology, Vol. 1 (Ed.: Spier, R.), Wiley-Interscience, pp. 293–320.

    Google Scholar 

  • Stacey, G.N. and Hartung, T. (2007) Availability, standardisation and safety of human cells and tissues for drug screening and testing. In: Drug Testing In Vitro: Breakthroughs and Trends in Cell Culture Technology (Eds.: Marx, U. and Sandig, V.), Wiley-VCH, New York, pp. 231–250.

    Google Scholar 

  • Stacey, G., Masters, J.R.W., Hay, R.J., Drexler, H.G., MacLeod, R.A.F., and Freshney, I.R. (2000) Cell contamination leads to inaccurate data: we must take action now. Nature, 403: 356.

    Article  PubMed  CAS  Google Scholar 

  • Stacey, G.N. and Phillips, P. (1999) Quality assurance for cell substrates. Dev. Standardisation, 98: 141–151.

    CAS  Google Scholar 

  • Stacey, A. and Stacey, G.N. (2000) Routine quality control testing for cell cultures. In: Methods in Molecular Medicine, Vol. 24: Antiviral Methods and Protocols (Eds.: Kinchington, D. and Schinazi, R.F.), Humana Press, Totowa, pp. 27–40.

    Google Scholar 

  • Stacey, G.N., Tyrrel, D.A., and Doyle, A. (1998) Source materials. In: Safety in Cell and Tissues Culture (Eds.: Stacey, G.N., Hambleton, P.J., and Doyle, A.), Kluwer Academic, Dordrecht, The Netherlands, pp. 1–25.

    Google Scholar 

  • Tautz, D. (1989) Hypervariability of simple sequences as a general source of polymorphic DNA markers. Nucleic Acids Res., 17: 6463–6471.

    Article  PubMed  CAS  Google Scholar 

  • Thomson, J., Itskovitz-Eldor, J., Shapiro, S., et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science, 282: 1145–1147.

    Article  PubMed  CAS  Google Scholar 

  • Tjio, J.H. and Leven, A. (1956) The chromosome number of men. Hereditas, 42: 1–6.

    Article  Google Scholar 

  • Uphoff, C.C., Gignac, S.M., and Drexler, H.G. (1992a) Mycoplasma contamination in human cell lines, II Elimination with various antibiotics. J. Immunol. Meth., 149: 55–62.

    CAS  Google Scholar 

  • Uphoff, C.C., Gignac, S.M., and Drexler, H.G. (1992b) Mycoplasma contamination in human cell lines, I Comparison of various detection methods. J. Immunol. Meth., 149: 43–53.

    CAS  Google Scholar 

  • US Food and Drugs Administration (2004a). 21 CFR Parts 16, 1270 and 1271. Current Good Tissue Practice for Human Cell, Tissue, and Cellular and Tissue-Based Products Establishments; Inspection and Enforcement; Final Rule. Department of Health and Human Services, November 24.

    Google Scholar 

  • US Food and Drug Administration (2004b) Title 21, Code of Federal Regulations, Part 210, Current Good Manufacturing Practice in Manufacturing, Processing, Packing, or Holding of Drugs; General, US FDA.

    Google Scholar 

  • US Food and Drug Administration (2004c) Title 21, Code of Federal Regulations, Part 211, Current Good Manufacturing Practice for Finished Pharmaceuticals, US FDA.

    Google Scholar 

  • US Food and Drugs Administration (2005a) Title 21, Code of Federal Regulations, Volume 7, revised April 2005, CFR610.12 (Sterility), FDA, Department of Health and Human Services.

    Google Scholar 

  • US Food and Drugs Administration (2005b) Title 21, Code of Federal Regulations, Volume 7, revised April 2005, CFR610.30 (Test for Mycoplama), FDA, Department of Health and Human Services.

    Google Scholar 

  • Van Kuppeveld, F.J.M., Johansson, K.-E., Galama, J.M.D., Kissing, J., Boelske, G., van der Logt, J.T.M., and Melchers, W.J.G. (1994) Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl. Env. Micro., 60: 149–152.

    Google Scholar 

  • Vassart, G., Georges, M., Monsieur, R., Brocas, H., Lequarre, A.S., and Christophe, D. (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science, 235: 683–684.

    Article  PubMed  CAS  Google Scholar 

  • Wagg, S.K. and Lee, L.E. (2005) A proteomics approach to identifying fish cell lines. Proteomics, 5: 4236–4244.

    Article  PubMed  CAS  Google Scholar 

  • Wang, H.C. and Federoff, S. (1972) Banding in human chromosomes treated with trypsin. Nat. New Biol., 235: 52–53.

    Article  PubMed  CAS  Google Scholar 

  • Wang, X.L., Wang, C.Y., Yu, X.J., Zhao, Y.S., Li, J., Duan, C.M., and Guo, X.M. (2005) Scalable production of embryoid bodies with the rotary cell culture system. Sheng Li Xue Bao. 57: 486–492.

    Article  PubMed  CAS  Google Scholar 

  • WFCC (1999) Guidelines for the Establishment and Operation of Collections of Cultures of Microorganisms, 2nd Edition, June 1999, http://wdcm.nig.ac.jp/wfcc/publication.html .

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Stacey, G.N. (2007). Quality Control of Human Stem Cell Lines. In: Masters, J.R., Palsson, B.O., Thomson, J.A. (eds) Human Cell Culture. Human Cell Culture, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5983-4_14

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