Skip to main content

Portable confocal scanning optical microscopy of Australopithecus africanus enamel structure

  • Chapter

Part of the book series: Vertebrate Paleobiology and Paleoanthropology ((VERT))

Abstract

The study of hominid enamel microanatomical features is usually restricted to the examination of fortuitous enamel fractures by low magnification stereo-zoom microscopy or, rarely, because of its intrusive nature, by high magnification compound microscopy of ground thin sections. To contend with limitations of magnification and specimen preparation, a Portable Confocal Scanning Optical Microscope (PCSOM) has been specifically developed for the non-contact and non-destructive imaging of early hominid hard tissue microanatomy. This unique instrument can be used for high resolution imaging of both the external features of enamel, such as perikymata and microwear, as well as internal structures, such as cross striations and striae of Retzius, from naturally fractured or worn enamel surfaces. Because there is veritably no specimen size or shape that cannot be imaged (e.g. fractured enamel surfaces on intact cranial remains), study samples may also be increased over what would have been possible before. We have applied this innovative technology to the study of enamel microanatomical features from naturally occurring occluso-cervical fractures of the South African hominid, Australopithecus africanus representing different tooth types. We present for the first time detailed information regarding cross striation periodicity for this species and, in addition, we present data on striae-EDJ angles in a large sample of teeth and crown formation time for a molar of A. africanus. Our results characterize a pattern of enamel development for A. africanus, which is different to that reported for the genus Paranthropus, as previously observed.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Berger, L., Lacruz, R.S., de Ruiter, D.J., 2002. Revised age estimates of Australopithecus bearing deposits at Sterkfontein, South Africa. American Journal of Physical Anthropology 119, 192-197.

    Article  Google Scholar 

  • Beynon, A.D., Dean, M.C., 1987. Crown formation time of a fossil hominid premolar tooth. Archives of Oral Biology 32, 773-780.

    Article  Google Scholar 

  • Beynon, A.D., Dean, M.C., 1988. Distinct dental development patterns in early fossil hominids. Nature 335, 509-514.

    Article  Google Scholar 

  • Beynon, A.D., Dean, M.C., Reid, D.J., 1991. A histological study on the chronology of the developing dentition of gorilla and orangutan. American Journal of Physical Anthropology 86, 295-309.

    Article  Google Scholar 

  • Beynon, A.D., Wood, B., 1986. Variations in enamel thickness and structure in East African hominids. American Journal of Physical Anthropology 70, 177-193.

    Article  Google Scholar 

  • Beynon, A.D., Wood, B., 1987. Patterns and rates of enamel growth on the molar teeth of early hominids. Nature 326, 493-496.

    Article  Google Scholar 

  • Boyde, A., 1964. The structure and development of mammalian enamel. Ph.D. Dissertation, University of London.

    Google Scholar 

  • Boyde, A., 1990. Developmental interpretations of dental microstructure. In: Jean de Rousseau, C. (Ed.), Primate Life History and Evolution. Wiley-Liss Publ., New York, pp. 229-267.

    Google Scholar 

  • Boyde, A., 1995. Confocal optical microscopy. In: Wootton, R., Springall, D.R., Polak, J.M. (Eds.), Image Analysis in Histology: Conventional and Confocal Microscopy. Cambridge University Press, Cambridge, UK, pp. 151-196.

    Google Scholar 

  • Boyde, A., Petran, M., Hadravsky, M., 1983. Tandem scanning reflected light microscopy of internal features in whole bone and tooth samples. Journal of Microscopy 132, 1-7.

    Article  Google Scholar 

  • Bromage, T.G., 1991. Enamel incremental periodicity in the pig-tailed macaque: a polychrome fluorescent labelling study of dental hard tissues. American Journal of Physical Anthropology 86, 205-214.

    Article  Google Scholar 

  • Bromage, T.G., Dean, M.C., 1985. Re-evaluation of the age at death of immature fossil hominids. Nature 317, 525-527.

    Article  Google Scholar 

  • Bromage, T.G., Perez-Ochoa, A., Boyde, A., 2003. The portable confocal microscope: scanning optical microscopy anywhere. In: Méndez Vilas, A. (Ed.), Science, Technology and Education of Microscopy: An Overview. Formatex, Badajoz, Spain, pp. 742-752.

    Google Scholar 

  • Bromage, T.G., Perez-Ochoa, A., Boyde, A., 2005. Portable confocal microscope reveals fossil hominid microstructure. Microscopic Analysis 19, 5-7.

    Google Scholar 

  • Dean, M.C., 1987. Growth layers and incremental markings in hard tissues, a review of the literature and some preliminary observations about enamel structure of Paranthropus boisei. Journal of Human Evolution 16, 157-172.

    Article  Google Scholar 

  • Dean, M.C., Beynon, A.D., Reid, D.J., Whittaker, D.K., 1993a. A longitudinal study of tooth growth in a single individual based on long and short period markings in dentine and enamel. International Journal of Osteoarchaeology 3, 249-264.

    Article  Google Scholar 

  • Dean, M.C., Beynon, A.D., hackeray, J.F., Macho, G.A., 1993b. Histological reconstruction of dental development and age at death of a juvenile Paranthropus robustus specimen, SK 63, from Swartkrans, South Africa. American Journal of Physical Anthropology 91, 401-419.

    Article  Google Scholar 

  • Dean, M.C., Leakey, M., Reid, D., Schrenk, F., Schwartz, G., Stringer, C., Walker, A., 2001. Growth processes in teeth distinguish modern humans from Homo erectus and earlier hominins. Nature 44, 628-631.

    Article  Google Scholar 

  • Dean, M.C., Reid, D.J., 2001. Perikymata and distribution on Hominid anterior teeth. American Journal of Physical Anthropology 116, 209-215.

    Article  Google Scholar 

  • Grine, F.E., Martin, L.B., 1988. Enamel thickness and development in Australopithecus and Paranthropus. In: Grine, F.E. (Ed.), The Evolutionary History of the ‘‘Robust’’ Australopithecines. Aldine de Gruyter, New York, pp. 3-42.

    Google Scholar 

  • Kino, G.S., 1995. Intermediate optics in Nipkow disk microscopes. In: Pawley, J.B. (Ed.), Handbook of Biological Confocal Microscopy. Plenum Press, New York, pp. 155-165.

    Chapter  Google Scholar 

  • Kraus, B.S., Jordan, R.E., 1965. The human dentition before birth. Lea & Febiger Publ., Philadelphia.

    Google Scholar 

  • Macho, G.A., Wood, B.A., 1995. The role of time and timing in hominid dental evolution. Evolutionary Anthropology 4, 17-31.

    Article  Google Scholar 

  • Nipkow, P., 1884. Elektrisches teleskop. Patentschrift 30105 (Kaiserliches Patentamt, Berlin), patented 06.01.1884.

    Google Scholar 

  • Petran, M., Hadravsky, M., 1966. Method and arrangement for improving the resolving power and contrast. United States Patent No. 3,517,980, priority 05.12.1966, patented 30.06.1970 US.

    Google Scholar 

  • Ramirez Rozzi, F., 1993. Tooth development in East African Paranthropus. Journal of Human Evolution 24, 429-454.

    Article  Google Scholar 

  • Ramirez Rozzi, F., 1998. Can enamel microstructure be used to establish the presence of different species of Plio-Pleistocene hominids from Omo, Ethiopia? Journal of Human Evolution 35, 543-576.

    Article  Google Scholar 

  • Ramirez Rozzi, F., 2002. Enamel microstructure in hominids: New characteristics for a new paradigm. In: Minugh-Purvis, N., McNamara, K.J. (Eds.), Human Evolution Through Developmental Change. Johns Hopkins University Press, Baltimore, pp. 319-348.

    Google Scholar 

  • Reid, D.J., Beynon, A.D., Ramirez Rozzi, F.V., 1998a. Histological reconstruction of dental development in four individuals from a Medieval site in Picardie, France. Journal of Human Evolution 35, 463-478.

    Article  Google Scholar 

  • Reid, D.J., Schwartz, G.T., Dean, M.C., Chandrasekera, M.S., 1998b. A histological reconstruction of dental development in the common chimpanzee. Journal of Human Evolution 35, 427-448.

    Article  Google Scholar 

  • Risnes, S., 1986. Enamel apposition rate and the prism periodicity in human teeth. Scandinavian Journal of Dental Research 94, 394-404.

    Google Scholar 

  • Schwartz, G.T., Liu, W., Zheng, L. (2003). Preliminary investigation of dental microstructure in the Yuanmou hominoid (Lufengpithecus hudienensis), Yumn Province, China. Journal of Human Evolution 44, 189-202.

    Article  Google Scholar 

  • Smith, T.M., 2004. Incremental development of primate dental enamel. Ph.D. Dissertation, State University of New York, Stony Brook.

    Google Scholar 

  • obias, P.V., 1980. Australopithecus afarensis and A. africanus: critique and an alternative hypothesis. Palaeontologica Africa 23, 1-17.

    Google Scholar 

  • Vrba, E.S., 1995. The fossil record of African antelopes (Mammalia, Bovidae) in relation to human evolution and paleoclimate. In: Vrba, E.S. (Ed.), Paleoclimate and Evolution, With Emphasis on Human Origins. Yale University Press, New Haven, pp. 385-424.

    Google Scholar 

  • White, T.D., Johanson, D.C., Kimbel, W.H., 1981. Australopithecus africanus: its phyletic position reconsidered. South African Journal of Science 77, 445-471.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to T.G. Bromage .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2007 Springer

About this chapter

Cite this chapter

Bromage, T., Lacruz, R., Perez-Ochoa, A., Boyde, A. (2007). Portable confocal scanning optical microscopy of Australopithecus africanus enamel structure. In: Bailey, S.E., Hublin, JJ. (eds) Dental Perspectives on Human Evolution: State of the Art Research in Dental Paleoanthropology. Vertebrate Paleobiology and Paleoanthropology. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5845-5_13

Download citation

Publish with us

Policies and ethics