Abstract
Some Brazilian soils present high contents of hardly soluble iron and aluminum phosphates and a high capacity for fixation of soluble phosphates. This study evaluated the ability of the fungus Aspergillus niger F111 isolated from soil to solubilize Fe and Al phosphates. Iron, aluminum or calcium phosphate were added to soil samples and inoculated with the A. niger F111. Sugar-cane molasses (2% v/w) was added as a carbon source on the 1st and 10th day of incubation. Soil samples without molasses, phosphates or fungus were used as control. Soil was incubated at 30 °C for twenty days and samples were removed every 5 days for determination of soil respiration (CO2 production), pH, titratable acidity, soluble phosphate and total carbohydrate contents. Soil respiration increased early on the first day after molasses addition and decreased thereafter to the minimum level. The largest contents of soluble phosphorus were observed on the 5th and 15th day of incubation, with the following sequence of phosphate solubilization: aluminum phosphate > iron phosphate > calcium phosphate > control. Another experiment was performed under the same conditions as described above using only aluminum phosphate as a source of phosphorus, with evaluations performed daily for 15 days. Aluminum phosphate solubilization was related to CO2 evolution, which increased on the 2nd and 12th day of incubation. Soluble phosphate increased on the 2nd and 11th day and titratable acidity increased on the 3rd and 11th day. Carbohydrates decreased after molasses application. The effect of solubilization of insoluble phosphates by the fungus depended on the addition of a carbon source (molasses) but decreased as soon as the carbon source was mineralized in the soil.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ae N, Arihara J, Okada K, Yoshihara T and Johansen C 1990 Phosphorus uptake by pigeon pea and its role in cropping systems of the Indian subcontinent. Science 248, 477–480.
Ajwa H A, Rice C W and Sotomayor D 1998 Carbon and nitrogen mineralization in tallgrass prairie and agricultural soil profiles. Soil Sci. Soc. Am. J. 62, 942–951.
Ames B N 1966 Assay of inorganic phosphate and phosphatases. Meth. Enzymol. 8, 115–116.
Angers D A and Mehuys G R 1989 Effects of cropping on carbohydrate content and water-stable aggregation of a clay soil. Can. J. Soil Sci. 69, 373–380.
Barroso C B and Nahas E. 2002 Unpublished results.
Bernal M P, Sánchez-Monedero M A, Paredes C and Roig A 1998 Carbon mineralization from organic wastes at different composting stages during their incubation with soil. Agric. Ecosystems and Environ. 69, 175–189.
Cerezine P C, Nahas E and Banzatto D A 1988 Soluble phosphate accumulation by Aspergillus niger from fluorapatite. Appl. Microbiol. Biotechnol. 29, 501–505.
Fageria N K and Baligar V C 2001 Improving nutrient use efficiency of annual crops in Brazilian acid soils for sustainable crop production. Commun. Soil Sci. Plant Anal. 32, 1303–1319.
Falih A M K and Wainwright M 1996 Microbial and enzyme activity in soils amended with a natural source of easily available carbon. Biol. Fertility Soils 21, 177–183.
Hattori A 1988 Microbial activities in soil amended with sewage sludges. Soil Sci. Plant Nutr. 43, 221–232.
Hayman D S 1975 Phosphorus cycling by soil micro-organisms and plant roots. In Soil Microbiology. Ed. N Walker. pp. 67–91. Butterworths, London.
Illmer P, Barbato A and Schinner F 1995 Solubilization of hardly soluble \( A1PO\underline {_4 } \) with P-solubilizing microorganisms. Soil Biol. Biochem. 27, 265–270.
Jones R D 2000 Phosphorus cycle. In Encyclopedia of Microbiology, Vol. 3, 2nd edn. Ed. J Lederberg. pp. 614–617. Academic, San Diego.
Marstorp H and Witter E 1999 Extractable dsDNA and product formation as measures of microbial growth in soil upon substrate addition. Soil Biol. Biochem. 31, 1443–1453.
Metzger L, Levanon D and Mingelgrin U 1987 The effect of sewage sludge on soil structural stability: microbiological aspects. Soil Sci. Soc. Am. J. 51, 346–351.
Nahas E 1996 Factors determining rock phosphate solubilization by microorganisms isolated from soil. World J. Microbiol. Biotechnol. 12, 567–572.
Nahas E, Banzatto D A and Assis L C 1990 Fluorapatite solubilization by Aspergillus niger in vinasse medium. Soil. Biol. Biochem. 22, 1097–1101.
Nahas E, Centurion J F and Assis L C 1994 Phosphatesolubilizing and phosphatase-producing microorganisms from various soils. Rev. Bras. Ci. Solo 18, 43–48.
Nahas E 2002 Factors affecting the solubilization of insoluble phosphates. In First International Meeting on Microbial Phosphate Solubilization. Ed. University of Salamanca IRNA-CSIC. pp. 20–22. Salamanca, Spain, 16–19 July 2002.
Pal S S 1998 Interactions of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant Soil. 198, 169–177.
Prado-Filho L G, Domingos R N and Silva S M G 1998 Acúmulo de cádmio por Saccharomyces cerevisiae fermentando mosto de melaço. Sci. Agric. 55, 128–132.
Reyes I, Bernier L, Simard R R and Antoun H 1999 Effect of nitrogen source on the solubilization of different inorganic phosphates by na isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microb. Ecol. 28, 281–290.
Richardson A E 2002 Making microorganisms mobilize soil phosphorus. In First International Meeting on Microbial Phosphate Solubilization. Ed. Univ. Salamanca IRNA-CSIC pp. 3–8. Salamanca, Spain. 16–19 July 2002.
SAS Institute 1990 Statistical Analysis System, SAS/STAT use’s guide (Version 6), 3rd edn. SAS Institute, Cary N.C. 705 pp.
Singh C P and Amberger A 1991 Solubilization and availability of phosphorus during decomposition of rock phosphate enriched straw and urine. Biol. Agric. Hort. 7, 261–269.
Stevenson F J and Cole M A 1999 Cycles of Soil: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients, 2nd edn. Wiley, New York. 448 pp.
Sundara B, Natarajan V and Hari K 2002 Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yields. Field Crop Res. 77, 43–49.
Tardieux-Roche A 1966 Contribution a l’étude des interactions entre phosphates naturels et microflore du sol. Ann. Agron. 17, 403–471.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Barroso, C.B., Nahas, E. (2007). Solubilization of hardly soluble iron and aluminum phosphates by the fungus Aspergillus niger in the soil. In: Velázquez, E., Rodríguez-Barrueco, C. (eds) First International Meeting on Microbial Phosphate Solubilization. Developments in Plant and Soil Sciences, vol 102. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5765-6_29
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5765-6_29
Received:
Accepted:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-4019-1
Online ISBN: 978-1-4020-5765-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)