• L.A. Manukyan
  • V.A. Ayvazyan
  • A.S. Boyajyan
Part of the NATO Security through Science Series book series


Cryoglobulins (Cgs) are pathogenic immune complexes, non specific markers of the inflammatory and autoimmune responses. In this study we for the first time, revealed Cgs in the blood of ischemic stroke patients and analyze their composition.


Ischemic Stroke Cerebral Ischemia Acute Ischemic Stroke Ischemic Stroke Patient Transient Cerebral Ischemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    U. Kallemuchikkal, P.D. Gorevic. Evaluation of cryoglobulins. Archives of Pathology and Laboratory Medicine, 123(2), 119–125 (1998)Google Scholar
  2. 2.
    A. Dispenzieri. Symptomatic cryoglobulinemia. Current Treatment Options in Oncology, 1(2), 105–118 (2000)CrossRefGoogle Scholar
  3. 3.
    K. Mohammed, H.U. Rehman. Cryoglobulinaemia. Acta Med. Austriaca, 30(3), 65–68 (2003)Google Scholar
  4. 4.
    P. Hilgard et al. Cryoglobulin-associated uptake of hepatitis C virus into human hepatocytes. Hepatogastroenterology, 52(65), 1534–1540 (2005)Google Scholar
  5. 5.
    C. Ferri et al. Cryoglobulins. J. Clin. Pathol., 55(1), 4–13 (2002)Google Scholar
  6. 6.
    M.M. Newkirk. Rheumatoid factors: host resistance or autoimmunity? Clinical Immunology, 104(1), 1–13 (2002)CrossRefGoogle Scholar
  7. 7.
    J.O. Brouet. et al. Biologic and clinical significance of cryoglobulins. A report of 86 cases. Am. J. Med., 57(5), 775–788 (1974)CrossRefGoogle Scholar
  8. 8.
    C. Ferri, M.T. Mascia. Cryoglobulinemic vasculitis. Curr Opin Rheumatol., 18(1), 54–63 (2006)CrossRefGoogle Scholar
  9. 9.
    B. Hellmich. et al. Early diagnosis of vasculitides. Z. Rheumatol., 64(8), 538–546 (2005)CrossRefGoogle Scholar
  10. 10.
    S.M. Weiner et al. Occurrence of C-reactive protein in cryoglobulins. Clin. Exp. Immunol., 125(2), 316–322 (2001)CrossRefMathSciNetGoogle Scholar
  11. 11.
    L. Mathsson et al. Cryoglobulin-induced cytokine production via FcgammaRIIa: inverse effects of complement blockade on the production of TNF-alpha and IL-10. Implications for the growth of malignant B-cell clones. Br. J. Haematol., 129(6), 830–838 (2005)CrossRefGoogle Scholar
  12. 12.
    P. Vilela, A. Goulao. Ischemic stroke: carotid and vertebral artery disease. Eur. Radiol., 15(3), 427–433 (2005)CrossRefGoogle Scholar
  13. 13.
    U.S. Vasthare et al. Complement depletion improves neurological function in cerebral ischemia. Brain Research Bulletin, 45(4), 413–419 (1998)CrossRefGoogle Scholar
  14. 14.
    A.L. D‘Ambrosio et al. The role of the complement cascade in ischemia/reperfusion injury: implications for neuroprotection. Molecular Medicine, 7(6), 367–382 (2001)MathSciNetGoogle Scholar
  15. 15.
    M.D. Ginsberg. Adventures in the pathophysiology of brain ischemia: penumbra, gene expression, neuroprotection: the 2002 Thomas Willis Lecture. Stroke, 34(1), 214–223 (2003)CrossRefMathSciNetGoogle Scholar
  16. 16.
    A. Boyajyan et al. Involvement of alternative and classical pathways of complement activation in the pathogenesis of ischemic stroke. Clinical Biochemistry, 38(9), 857–858 (2005)Google Scholar
  17. 17.
    A.S. Boyajyan et al. Dynamics of complement activation in acute ischemic stroke. Immunology (Moscow), 25(4), 221–224 (2004)Google Scholar
  18. 18.
    K. Saito et al. Early increases in TNF-alpha, IL-6 and IL-1 beta levels following transient cerebral ischemia in gerbil brain. Neurosci. Lett., 206(2–3), 149–152 (1996)CrossRefGoogle Scholar
  19. 19.
    L. Zheng et al. Induction of apoptosis in mature T cells by tumor necrosis factor. Nature 377(6547), 348–351 (1995)CrossRefADSGoogle Scholar
  20. 20.
    J.A. Hardin. Cryoprecipitogogue from normal serum: mechanism for cryoprecipitation of immune complexes. Proc. Natl. Acad. Sci. USA, 78(7), 4562–4565 (1981)CrossRefADSGoogle Scholar
  21. 21.
    L. Musset et al. Characterization of cryoglobulins by immunoblotting. Clin.Chem., 38, 798–802 (1992)Google Scholar
  22. 22.
    O.H. Lowry et al. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193(1), 265–275 (1951)Google Scholar
  23. 23.
    D. Siedel et al. Improved techniques for assessment of plasma lipoprotein patterns. I. Precipitation in gels after electrophoresis with polyanionic compounds. Clin.Chem., 19(7), 737–739 (1973)Google Scholar
  24. 24.
    Mini-Protean 3 Cell. Instruction Mannual (catalog number 165–3301), Bio-Rad Laboratories, Inc., 1–23Google Scholar
  25. 25.
    Mini Trans-Blot Electrophoretic Transfer Cell. Instruction Mannual (catalog number 170–3930), Bio-Rad Laboratories, Inc., 1–24Google Scholar
  26. 26.
    G. Stoll. Inflammatory cytokines in the nervous system: multifunctional mediators in autoimmunity and cerebral ischemia. Inflammatory cytokines in the nervous system: multifunctional mediators in autoimmunity and cerebral ischemia. Rev. Neurol. (Paris), 158(10 Pt 1), 887–891 (2002)Google Scholar
  27. 27.
    K.J. Becker et al. Sensitization to brain antigens after stroke is augmented by lipopolysaccharide. J. Cereb. Blood Flow Metab., 25(12), 1634–1644 (2005)CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • L.A. Manukyan
    • 1
  • V.A. Ayvazyan
    • 1
  • A.S. Boyajyan
    • 1
  1. 1.Laboratory of Macromolecular ComplexesInstitute of Molecular Biology NAS RAYerevanArmenia

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