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THERMODYNAMIC PROFILE OF INTERACTION OF PORPHYRINS WITH NUCLEIC ACIDS

  • Samvel Haroutiunian
  • Yeva Dalyan
  • Ara Ghazaryan
  • Tigran Chalikian
Conference paper
Part of the NATO Security through Science Series book series

Abstract

The binding of a number of novel water soluble porphyrins to nucleic acids (NA) were studied monitoring the changes in Soret region of absorbance spectra, fluorescence spectra and circular dichroism (CD) spectra of formed complexes. The binding modes for all complexes were dtermined. The binding isotherms were used to calculate the binding constant, Kb, and binding free energy, ⊿Gb = -RTlnKb. By performing these experiments as a function of temperature, we evaluated the van’t Hoff binding enthalpies, ⊿Hb and the binding entropies, ⊿Sb. Generalization concerning the established correlation between the binding mode and thermodynamic profile was done.

Keywords

Circular Dichroism Binding Mode Circular Dichroism Spectrum Favorable Change Unfavorable Change 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2007

Authors and Affiliations

  • Samvel Haroutiunian
    • 1
  • Yeva Dalyan
    • 1
  • Ara Ghazaryan
    • 1
  • Tigran Chalikian
    • 2
  1. 1.Department of Molecular PhysicsYerevan State UniversityYerevanArmenia
  2. 2.Department of Pharmaceutical Sciences, Leslie Dan Faculty of PharmacyUniversity of TorontoTorontoCanada

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