Abstract
The purpose of this review is twofold: first, to recall some modern emission methods in biochemistry and biophysics and second, to furnish their scope and limitations. Methodology of this part is based on the shift between excitation and emission spectra (Stokeś shift) and fluorescence anisotropy, including advantages of multi-photon excitations. Their applications in confocal microscopy led to higher spatial and temporal resolution, and lower photodamages, thus permit dipper penetration of the cells and tissues, and a ultraprecise nanosurgical applications. Higher selectivity of two-photon excitation (TPE) of tryptophan vs tyrosine residues is attainable due to short-wavelength shift of the TPE spectrum of tyrosine, and zero or slightly negative values of its TPE fundamental anisotropy.
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KIERDASZUK, B. (2007). EMISSION SPECTROSCOPY IN BIOPHYSICAL MACROMOLECULAR RESEARCH. In: Tsakanov, V., Wiedemann, H. (eds) Brilliant Light in Life and Material Sciences. NATO Security through Science Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5724-3_16
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DOI: https://doi.org/10.1007/978-1-4020-5724-3_16
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