Conference paper
Part of the NATO Science Series book series (NAIV, volume 76)


A new solvent-responsive gene locus, designated sepABC and a divergently transcribed sepR, (sep for solvent efflux pump), was found downstream of the two-component todST signal transduction phosphorelay system that regulates toluene degradation (the tod pathway) in Pseudomonas putida F1 (PpF1). We have made use of this new property, a non-catabolic promoter, in combination with a luxCDABE gene cassette to create a second generation whole-cell bioluminescent biosensor, of name PpF1G4. The response of this new biosensor to a wide range of aromatic hydrocarbons as well as a number of ubiquitous multicomponent non-aqueous phase liquids (NAPLs), including gasoline, JP-4 jet fuel, diesel, coal tar creosote and three varieties of crude oil was demonstrated. PpF1G4 was also assessed as a new environmental tool for a direct measurement of the bioavailability of hydrophobic organic compounds (HOCs) partitioned into surfactant micelles. In general, the results of this study demonstrated the utility of a novel bioreporter system capable of direct measurement of bioavailability of HOCs by the judicious choice of non-ionic surfactants.


Critical Micelle Concentration Nonionic Surfactant Pseudomonas Putida Micellar Phase Bioluminescence Assay 
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Copyright information

© Springer 2007

Authors and Affiliations

  1. 1.Department of Civil EngineeringMcGill UniversityMontreal, QuebecCanada
  2. 2.Biotechnology Research InstituteNational Research Council CanadaMontreal, QuebecCanada

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