Closed Loop Control of Perfusion Systems in High-density Cell Culture

  • A. Bock
  • U. Reichl
Conference paper


The use of a perfusion system prevented unspecific inhibitions effects on cell growth for batch cultivations of adherent MDCK cells used for an equine influenza A vaccine production process. It also increased viable cell numbers. An enzyme sensor for monitoring and closed loop control of metabolites operated stable for at least 55 hours.


MDCK Cell None None Perfusion System Viable Cell Number Perfusion Cultivation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    Genzel, Y., Behrendt, I., König, S., Sann, H., Reichl, U., 2002, Metabolism of MDCK cells during growth and Influenza virus production in large-scale microcarrrier culture; Vaccine 22(17–18): 2202–2208.Google Scholar
  2. [2]
    Glacken, M. W., Fleischaker, R. J., Sinskey, A. J., 1985, Reduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells, Biotech. Bioeng. 28: 1376–1389.CrossRefGoogle Scholar
  3. [3]
    Hauser, H., Wagner, R., 1997, Mammalian cell biotechnology in protein production, W. de Gruyther.Google Scholar
  4. [4]
    Möhler, L., Flockerzi, D., Sann H., Reichl, U., 2005, A mathematical model of Influenza A virus production in large-scale microcarrier culture; Biotech. Bioeng. 90 (1): 46–58.CrossRefGoogle Scholar
  5. [5]
    Sann, H., Bock, A., Reichl, U., 2002, Device and method for extracting liquid samples, WO 2004/ 033077 A2.Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • A. Bock
    • 1
  • U. Reichl
    • 1
    • 2
  1. 1.Max Planck Institute for Dynamics of Complex Technical Systems MagdeburgMagdeburgGermany
  2. 2.Otto-von-Guericke-University MagdeburgMagdeburgGermany

Personalised recommendations