Closed Loop Control of Perfusion Systems in High-density Cell Culture

  • A. Bock
  • U. Reichl
Conference paper

Abstract

The use of a perfusion system prevented unspecific inhibitions effects on cell growth for batch cultivations of adherent MDCK cells used for an equine influenza A vaccine production process. It also increased viable cell numbers. An enzyme sensor for monitoring and closed loop control of metabolites operated stable for at least 55 hours.

Keywords

Lactate Influenza Aeration 

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References

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    Genzel, Y., Behrendt, I., König, S., Sann, H., Reichl, U., 2002, Metabolism of MDCK cells during growth and Influenza virus production in large-scale microcarrrier culture; Vaccine 22(17–18): 2202–2208.Google Scholar
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    Sann, H., Bock, A., Reichl, U., 2002, Device and method for extracting liquid samples, WO 2004/ 033077 A2.Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • A. Bock
    • 1
  • U. Reichl
    • 1
    • 2
  1. 1.Max Planck Institute for Dynamics of Complex Technical Systems MagdeburgMagdeburgGermany
  2. 2.Otto-von-Guericke-University MagdeburgMagdeburgGermany

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