Closed Loop Control of Perfusion Systems in High-density Cell Culture
The use of a perfusion system prevented unspecific inhibitions effects on cell growth for batch cultivations of adherent MDCK cells used for an equine influenza A vaccine production process. It also increased viable cell numbers. An enzyme sensor for monitoring and closed loop control of metabolites operated stable for at least 55 hours.
KeywordsMDCK Cell None None Perfusion System Viable Cell Number Perfusion Cultivation
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