Abstract
This work aims to investigate the changes occurring in metabolism of a cell population during adaptation of a cell line from serum containing to serum free media. In order to enable growth in a serum free media a supplement was added to DME:F12. Cells grown in 1.25% serum media were used to investigate the effect of the supplement, data for growth, antibody production and key metabolites are presented All data presented here are derived from cells banked at different serum concentrations during the adaptation and subsequently grown in 50mL culture in 250mL shaken ehrlenmyer flasks.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Denby, S., Baganz, F., Mousdale, D. (2007). Metabolic Changes Occuring During Adaptation to Serum Free Media. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_81
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_81
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)