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Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody

  • Cristina A. Caldas
  • Diorge P. Souza
  • Maria Teresa A. Rodrigues
  • Andréa Q. Maranhão
  • Ana M. Moro
  • Marcelo M. Brigido
Conference paper

Abstract

In this work the closest human germline sequence was used as the framework on to which to graft murine CDRs. The proposed fully humanized version displays the same staining pattern to different cell subpopulations like CD4, CD8 and memory CD45RO lymphocytes, when compared with the original anti-CD18 MAb, reinforcing the germline approach as a successful strategy to humanize antibodies with maintenance of affinity and selectivity.

Keywords

Leucine Residue Germline Sequence Murine Origin Humanize scFv Human Lymphocyte Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2007

Authors and Affiliations

  • Cristina A. Caldas
    • 1
    • 4
  • Diorge P. Souza
    • 2
  • Maria Teresa A. Rodrigues
    • 3
    • 4
  • Andréa Q. Maranhão
    • 2
    • 4
  • Ana M. Moro
    • 3
    • 4
  • Marcelo M. Brigido
    • 2
    • 4
  1. 1.Laboratório de Imunologia, InCor, USPBrasil
  2. 2.Laboratório de Biologia Molecular, UnBBrasi
  3. 3.Laboratório Biofármacos em Célula AnimalInstituto ButantanBrasil
  4. 4.Instituto de Investigação em Imunologia - iii, Institutos do MilênioBrasil

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