Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production
The expression of retinoic acid receptor (RAR) and retinoid X receptor (RXR) was compared between RA responsive and unresponsive hybridoma cell lines. RT-PCR analysis showed that most of cell lines treated with RA expressed the RAR-alpha, -beta and -gamma, and the RXR-alpha and -beta. Interestingly, RXR-alpha was little expressed in the unresponsive cell lines. In addition, the transfection of RXR-alpha siRNA to the responsive cell line significantly suppressed the increasing effect of RA, suggesting that RXR-alpha may be associated with MAb up-regulation by RA. The RXR or RAR selective ligand alone and their combinations all could increase MAb production. Their abilities to increase the MAb production were almost paralleled with those to induce the expression of the RAR/RXR-mediated gene, RAR-beta, suggesting that MAb production may be up-regulated through, in part, the RAR/RXR heterodimers. Taken together, our results suggest that RXR-alpha may play an important role in MAb up-regulation through the RAR/RXR heterodimers
KeywordsPeroxide Agarose Bromide Electrophoresis Ethidium
Unable to display preview. Download preview PDF.
- Kawahara, H., Shirahata, S., Tachibana, H. and Murakami, H., 1992, In vitro immunization of human lymphocytes with human lung cancer cell line A549, Hum. Antibod. Hybridomas 3: 8–13.Google Scholar
- Shoji, M., Kawamoto, S., Sato, S., et al., 1994, Specific reactivity of human antibody AE6F4 against cancer cells in tissues and sputa from lung cancer patients, Hum. Antibod. Hybridoma. 5: 116–122.Google Scholar