Advertisement

Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production

  • Yuichi Inoue
  • Hiroharu Kawahara
  • Sanetaka Shirahata
  • Yasushi Sugimoto
Conference paper

Abstract

The expression of retinoic acid receptor (RAR) and retinoid X receptor (RXR) was compared between RA responsive and unresponsive hybridoma cell lines. RT-PCR analysis showed that most of cell lines treated with RA expressed the RAR-alpha, -beta and -gamma, and the RXR-alpha and -beta. Interestingly, RXR-alpha was little expressed in the unresponsive cell lines. In addition, the transfection of RXR-alpha siRNA to the responsive cell line significantly suppressed the increasing effect of RA, suggesting that RXR-alpha may be associated with MAb up-regulation by RA. The RXR or RAR selective ligand alone and their combinations all could increase MAb production. Their abilities to increase the MAb production were almost paralleled with those to induce the expression of the RAR/RXR-mediated gene, RAR-beta, suggesting that MAb production may be up-regulated through, in part, the RAR/RXR heterodimers. Taken together, our results suggest that RXR-alpha may play an important role in MAb up-regulation through the RAR/RXR heterodimers

Keywords

Retinoic Acid Retinoic Acid Receptor ERDF Medium Increase Antibody Production Responsive Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aotsuka, Y. and Naito, M., 1991, Enhancing effects of retinoic acid on monoclonal antibody production of human-human hybridomas, Cell. Immunol. 133: 498–505.CrossRefGoogle Scholar
  2. Ballow, M., Wang, X., Xiang, S. and Allen, C., 2003, Expression and regulation of nuclear retinoic acid receptors in human lymphoid cells, J. Clin. Immunol. 23: 46–54.CrossRefGoogle Scholar
  3. Inoue, Y., Fujisawa, M., Shoji, M., et al., 2000, Enhanced antibody production of humanhuman hybridomas by retinoic acid, Cytotechnology 33: 83–88.CrossRefGoogle Scholar
  4. Inoue, Y. and Shirahata, S., 2002, Increased antibody production by retinoids is related to the fusion partner of human hybridomas, Biosci. Biotechnol. Biochem., 66 (1): 215–217.CrossRefGoogle Scholar
  5. Kawahara, H., Shirahata, S., Tachibana, H. and Murakami, H., 1992, In vitro immunization of human lymphocytes with human lung cancer cell line A549, Hum. Antibod. Hybridomas 3: 8–13.Google Scholar
  6. Murakami, H., Hashizume, S., Ohashi, H., et al., 1985, Human-human hybridomas secreting anibodies specific to human lung carcinoma, In Vitro Cell. Dev. Biol., 21: 593–596.CrossRefGoogle Scholar
  7. Shoji, M., Kawamoto, S., Sato, S., et al., 1994, Specific reactivity of human antibody AE6F4 against cancer cells in tissues and sputa from lung cancer patients, Hum. Antibod. Hybridoma. 5: 116–122.Google Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Yuichi Inoue
    • 1
  • Hiroharu Kawahara
    • 4
  • Sanetaka Shirahata
    • 3
  • Yasushi Sugimoto
    • 2
  1. 1.Faculty of AgricultureKagoshimaJapan
  2. 2.The United Graduate School of Agricultural SciencesKagoshima UniversityKagoshimaJapan
  3. 3.Division of Life Engeering,Graduate School of Systems Life SciencesKyushu UniversityFukuokaJapan
  4. 4.Kitakyushu National College of TechnologyKitakyushuJapan

Personalised recommendations