Abstract
Dendritic cells (DC) represent the antigen-presenting cells which play a decisive role in the regulation of immune responses. Large numbers of monocyte-derived DC can be generated from leukapheresis cells for clinical applications by positive selection of CD14+ cells or by negative selection (removal of CD2+ and CD19+ cells). The positive selection showed a lower yield but higher purity of monocytes. Additionally, we compared the culture flask system (as “gold standard„) with our integrated-closed-bag system designed for clinical use. Bags resulted in slightly lower yields of viable cells on day 7 compared to flasks. Structural and functional properties of DC generated by these cultivation conditions showed no significant difference. A high donor variability was observed. In regard to clinical application and GMP compliance CD14+ selection in combination with our closed-bag system is a promising approach to generate defined dendritic cells.
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Macke, L. et al. (2007). Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_38
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_38
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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