Abstract
Animal derived protein hydrolysates are commonly used as a complex supplement for cell culture media because of their well known growth promoting properties. In an approach to find components being responsible for this effect we fractionated a protein hydrolysate by solid phase extraction (SPE) on a C18-matrix. After lyophilization and further preparation the fractions were tested for their proliferation enhancing ability with a hybridoma cell line in a serum-free DMEM/F12 medium supplemented with human serum albumin and transferrin. We could demonstrate a growth promoting effect caused by a rather hydrophilic fraction. In further investigations preparative High Performance Liquid Chromatography (HPLC) was used to produce growth promoting fractions of less complexity for cell evaluation and analytical HPLC.
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Schomberg, M., Hakemeyer, C., Büntemeyer, H., Stiens, L., Lehmann, J. (2007). Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_25
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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