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Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells

  • Michael Schomberg
  • Christian Hakemeyer
  • Heino Büntemeyer
  • Lars Stiens
  • Jürgen Lehmann
Conference paper

Abstract

Animal derived protein hydrolysates are commonly used as a complex supplement for cell culture media because of their well known growth promoting properties. In an approach to find components being responsible for this effect we fractionated a protein hydrolysate by solid phase extraction (SPE) on a C18-matrix. After lyophilization and further preparation the fractions were tested for their proliferation enhancing ability with a hybridoma cell line in a serum-free DMEM/F12 medium supplemented with human serum albumin and transferrin. We could demonstrate a growth promoting effect caused by a rather hydrophilic fraction. In further investigations preparative High Performance Liquid Chromatography (HPLC) was used to produce growth promoting fractions of less complexity for cell evaluation and analytical HPLC.

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography Human Serum Albumin Solid Phase Extraction Protein Hydrolysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer 2007

Authors and Affiliations

  • Michael Schomberg
    • 1
  • Christian Hakemeyer
    • 1
  • Heino Büntemeyer
    • 1
  • Lars Stiens
    • 2
  • Jürgen Lehmann
    • 1
  1. 1.Institute of Cell Culture TechnologyUniversity of BielefeldBielefeldGermany
  2. 2.Roche Diagnostics GmbHPenzbergGermany

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