Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells
Animal derived protein hydrolysates are commonly used as a complex supplement for cell culture media because of their well known growth promoting properties. In an approach to find components being responsible for this effect we fractionated a protein hydrolysate by solid phase extraction (SPE) on a C18-matrix. After lyophilization and further preparation the fractions were tested for their proliferation enhancing ability with a hybridoma cell line in a serum-free DMEM/F12 medium supplemented with human serum albumin and transferrin. We could demonstrate a growth promoting effect caused by a rather hydrophilic fraction. In further investigations preparative High Performance Liquid Chromatography (HPLC) was used to produce growth promoting fractions of less complexity for cell evaluation and analytical HPLC.
KeywordsHigh Performance Liquid Chromatography Albumin Acetonitrile Fractionation Pyruvate
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