Adaptation without Cell Division?
The inducible expression of p21 CIP1 cyclin dependent kinase system in CHO cells has been shown to be capable of uncoupling cell growth and productivity enabling higher levels of productivity without increasing the overall cell number, however arrested cultures expressing p21 appear to proceed into the death phase in a relatively similar time span as non arrested cultures despite the differences in overall cell numbers. By combining p21 and Bcl-2 expression it was possible to maintain cells almost indefinitely in a state of cell cycle arrest through which it was possible to adapt cells to suspension and serum free environments at a much quicker and effective rate than that for the non arrested cells.
KeywordsIPTG Hygromycin Staurosporine
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