Adaptation without Cell Division?

Enhanced adaptation to suspension and serum free culture by the co-expression of p21 and Bcl-2
  • K.L. Astley
  • M. Al-Rubeai
Conference paper

Abstract

The inducible expression of p21 CIP1 cyclin dependent kinase system in CHO cells has been shown to be capable of uncoupling cell growth and productivity enabling higher levels of productivity without increasing the overall cell number, however arrested cultures expressing p21 appear to proceed into the death phase in a relatively similar time span as non arrested cultures despite the differences in overall cell numbers. By combining p21 and Bcl-2 expression it was possible to maintain cells almost indefinitely in a state of cell cycle arrest through which it was possible to adapt cells to suspension and serum free environments at a much quicker and effective rate than that for the non arrested cells.

Keywords

IPTG Hygromycin Staurosporine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Watanabe S., J. Shuttleworth and M. Al-Rubeai (2002) Regulation of cell cycle and productivity in NS0 cells by the over-expression of p21CIP1. Biotechnology and Bioengineering. 77, 1–7.CrossRefGoogle Scholar
  2. Ibarra N, Watanabe S, Bi,J-X, Shuttleworth J and Al-Rubeai M (2003) Modulation of Cell Cycle for Enhancement of Antibody Productivity in Perfusion Culture of NSO Cells. Biotechnology Progress. 19, 224–228.CrossRefGoogle Scholar
  3. Bi J.-X. Shuttleworth J., Al-Rubeai M. (2004) Uncoupling of cell growth and proliferation results in enhancement of productivity. Biotechnology & Bioengineering. 85, 741–749CrossRefGoogle Scholar
  4. Simpson N.H., Milner A.E. and Al-Rubeai M. (1997). Prevention of hybridoma cell death by Bcl-2 during suboptimal culture conditions. Biotechnol. Bioeng. 54: 1–16.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • K.L. Astley
    • 1
  • M. Al-Rubeai
    • 2
  1. 1.Department of Chemical EngineeringUniversity of BirminghamBirminghamUK
  2. 2.Department of Chemical and Biochemical EngineeringUniversity College DublinBelfieldIreland

Personalised recommendations