Screening Strategies for Iron Chelators in Serum Free Media
A variety of iron chelators, including recombinant human transferrin, were evaluated as replacements to holo human transferrin on a number of industrially relevant cell lines (MDCK, BHK-21 and Vero). The results showed that short term screening over one passage is not necessarily adequate to select a suitable transferrin replacement.
KeywordsMDCK Cell Iron Chelator Screening Assay Ferrous Sulphate Human Transferrin
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