Abstract
A variety of iron chelators, including recombinant human transferrin, were evaluated as replacements to holo human transferrin on a number of industrially relevant cell lines (MDCK, BHK-21 and Vero). The results showed that short term screening over one passage is not necessarily adequate to select a suitable transferrin replacement.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bradshaw GL, Sato GH, McClure DB and Dubes GR (1994). The growth requirements of BHK-21 in serum-free culture. J Cell Physiol. Feb 114(2); 215–21.
Darfler FJ (1990). A protein-free medium for the growth of hybridomas and other cells of the immune system. In Vitro 26, 769–778.
Gammell P., O.Driscoll L. and Clynes M. (2003). Characterisation of BHK-21-PPI-C16–21 cells engineered to secrete human insulin. Cytotechnol. 41: 11–21
Keenan J and Clynes M (1996). Replacement of transferring by simple iron compounds for MDCK cells grown and subcultured in serum-free medium. In Vitro Sep 32(8) 451–3.
Martin A and Clynes M (1993). Comparison of 5 microplate colorimetric assays for in vitro cytotoxicity testing and cell proliferation assays. Cytotechnology 11: 49–58.
O'Driscoll L., Gammell P. and Clynes M. (2002). Engineering Vero-PPI cells to secrete human Insulin. In Vitro 38: 146–153
Richardson DR and Ponka P (1997). The molecular mechanism of the metabolism and transport of iron in normal and neoplastic cells. BBA 1331, 1–40.
Taub M, Chuman L, Saier, MH, Sato G (1979). Growth of MDCK in hormonally defined serum-free medium. PNAS 76 (7): 3338–3342.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Keenan, J., Pearson, D., Clynes, M. (2007). Screening Strategies for Iron Chelators in Serum Free Media. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_144
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_144
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)