Erythropoietin Purification Employing Affinity Membrane Adsorbers

  • Maria Candida M. Mellado
  • David Curbelo
  • Ronaldo Nobrega
  • Leda R. Castilho

Abstract

In the present work, rhEPO was purified from crude CHO cell culture supernatant either with Sartobind-Cibacron Blue (CB) or Sartobind-IDA-Cu+2 affinity membranes. Purity degrees were of 55 and 75%, respectively.

Keywords

HPLC Convection Candida Erythropoietin 

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References

  1. Castilho, L.R., Anspach, F.B., Deckwer, W.-D., 2002, Comparison of affinity membranes for the purification of immunoglobulins, J. Membrane Sci. 207: 253–264.CrossRefGoogle Scholar
  2. Ghosh, R., 2002, Protein separation using membrane chromatography: opportunities and challenges, J. Chromatogr. A 952: 13–27.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Maria Candida M. Mellado
    • 1
  • David Curbelo
    • 2
  • Ronaldo Nobrega
    • 1
  • Leda R. Castilho
    • 1
  1. 1.Federal University of Rio de Janeiro (UFRJ), COPPE, Chemical Engineering ProgramRio de Janeiro/RJBrazil
  2. 2.Center of Molecular Immunology (CIM)HavanaCuba

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