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Erythropoietin Purification Employing Affinity Membrane Adsorbers

  • Maria Candida M. Mellado
  • David Curbelo
  • Ronaldo Nobrega
  • Leda R. Castilho

Abstract

In the present work, rhEPO was purified from crude CHO cell culture supernatant either with Sartobind-Cibacron Blue (CB) or Sartobind-IDA-Cu+2 affinity membranes. Purity degrees were of 55 and 75%, respectively.

Keywords

Cell Culture Supernatant Recombinant Human Erythropoietin Lower Manufacturing Cost Eluent Volume Molecular Immunology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Castilho, L.R., Anspach, F.B., Deckwer, W.-D., 2002, Comparison of affinity membranes for the purification of immunoglobulins, J. Membrane Sci. 207: 253–264.CrossRefGoogle Scholar
  2. Ghosh, R., 2002, Protein separation using membrane chromatography: opportunities and challenges, J. Chromatogr. A 952: 13–27.CrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Maria Candida M. Mellado
    • 1
  • David Curbelo
    • 2
  • Ronaldo Nobrega
    • 1
  • Leda R. Castilho
    • 1
  1. 1.Federal University of Rio de Janeiro (UFRJ), COPPE, Chemical Engineering ProgramRio de Janeiro/RJBrazil
  2. 2.Center of Molecular Immunology (CIM)HavanaCuba

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