GENOMIC APPROACHES IN VIRUS DIAGNOSTICS A PERSONAL ASSESSMENT OF REALITIES WHEN FACED WITH VIRUSES IN A PLANT BIOSECURITY CONTEXT
- 691 Downloads
During the past 30 years, most plant pathogenic viruses and viroids have been characterized in terms of their base sequence. A few, such as viruses in the family Luteoviridae, were harder to crack than others but recently yielded to this approach (e.g. Huang et al., 2005). With the primary base sequences of these viruses determined, it was possible to infer relationships but also to identify the positions of functional units. Additionally, it was often possible to unravel the complex interactions in time and space involved with genome expression. Thus, because of their relatively small genome sizes, viruses were in the vanguard of what has come to be grouped under the generic title “omic” technologies; the word “transcriptomics” was not used to describe these early technological thrusts but might be now.
KeywordsMolecular Beacon Nucleic Acid Hybridization Maize Streak Virus Turnip Mosaic Virus Nucleic Acid Spot Hybridization
Unable to display preview. Download preview PDF.
- Cooper, J.I., and M.L. Edwards, 1986. Variations and limitations of enzyme amplified immunoassays, in Developments and applications in Virus Testing, edited by R.A.C. Jones and L. Torrance, Association of Applied Biologists, Wellesbourne, U.K., pp. 139–154.Google Scholar
- Jackson, R.J., A.J. Ramsay, C.D. Christensen, S. Beaton, D.F. Hall, and I.A. Ramshaw, 2001. Expression of mouse interleukin-4 by a recombinant ectromelia virus suppresses cytolytic lymphocyte responses and overcomes genetic resistance to mousepox, J. Virol., 75, 1205–1210.PubMedCrossRefGoogle Scholar
- Jones, R.A.C., and L. Torrance, 1986. Developments in Applied Biology 1. Developments and Applications in Virus Testing, Association of Applied Biologists, Wellesbourne, U.K., 312 p.Google Scholar
- MacKenzie, D.J., M.A. McLean, S. Mukerji, and M. Green, 1997. Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription–polymerase chain reaction, Plant Dis., 81, 222–226.Google Scholar
- Robertson, N.L., and D.C. Ianson, 2005. First Report of Turnip mosaic virus in rhubarb in Alaska, Plant Dis., 89, 430.Google Scholar
- Tian, T., V.A. Klaasen, J. Soong, G. Wisler, J.E. Duffus, and B.W. Falk, 1996. Generation of cDNAs specific to lettuce infectious yellows closterovirus and other whitefly-transmitted viruses by RT-PCR and degenerate oligonucleotide primers corresponding to the closterovirus gene coding the heat shock protein, Phytopathology, 86, 1167–1173.CrossRefGoogle Scholar