CHARACTERIZATION OF THE FERM DOMAIN PROTEIN EHM2 IN HUMAN CANCER CELLS
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The FERM domain protein EHM2 (EPB41L4B) was first isolated and characterized based on its elevated expression in highly metastatic mouse melanoma cells. We recently found that human EHM2 is androgen-regulated in a cancer cell line model of steroid-induced cytoskeletal reorganization, and expression profiling analyses by others have shown that it is a primary steroidregulated gene in rat liver and human lung cells. Bioinformatic analysis of human EHM2 revealed that it is a member of a unique subfamily of FERM domain proteins that includes the Drosophila YURT gene. Analysis of YURT protein functions in Drosophila have shown that it is required for dorsal closure during embryogenesis and is involved in mediating epithelial cell migration. We have used immunostaining to analyze steroid-induced and ectopic expression of EHM2 in the human fibrosarcoma cell line HT-1080 and found that it is localized to the cell membrane and associated with cytoskeletal reorganization. We have also found that EHM2 is highly expressed in the metastatic prostate cancer cell lines LNCaP, DU-145 and PC-3 cells, and moreover, that EHM2 transcripts are present at significantly higher levels in human prostate tumors than in non-malignant prostate cells. Based on these data, we propose that elevated expression of EHM2 may enhance the metastatic properties of advanced prostate cancers.
KeywordsProstate Cancer Cell Line Guanine Nucleotide Exchange Factor Cytoskeletal Reorganization Ferm Domain Prostate Cancer Sample
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