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DETERMINATION OF PROTEIN COMPLEXED PARALYTIC SHELLFISH POISONING. MECHANISM OF THE REMEDIATION (DETOXIFICATION) OF CHEMICALS (PESTICIDE, HEAVY METALS, OTHER TOXIC CHEMICAL COMPOUNDS)

Purification and Characterization of Paralytic Shellfish Poison Binding Protein from Acanthocardia Tuberculatum

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Chemicals as Intentional and Accidental Global Environmental Threats

Part of the book series: NATO Security through Science Series ((NASTC))

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Abstract

A paralytic shellfish poison binding protein (PSPBP) was purified with 16.6 fold from the foot of the Moroccan cockles Acanthocardia tuberculatum. Using the affinity chromatography, 2.5 mg of PSPBP showing homogeneity on SDS-PAGE was obtained from 93 mg of crude extract. The purified PSPBP exhibits a specific activity of 2.777 mU/mg proteins and having estimated molecular weight of 181 kDa. Observation of single band equivalent to 88 kDa on SDS-PAGE under reducing conditions suggested it to be a homodimer. The optimal temperature and pH for the purified PSPBP were 30 °C and 7.

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Author information

Authors and Affiliations

  1. Laboratory of Microbiology, Biotechnology and Environment, Faculty of Sciences Aïn Chock, University Hassan II-Aïn Chock, Km 8 route d’El Jadida, B.P. 5366 Mâarif, 5366, Casablanca, Morocco

    NADIA TAKATI & MOHAMED BLAGHEN

  2. Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences Aïn Chock, University Hassan II-Aïn Choc, Casablanca, Morocco

    DRISS MOUNTASSIF

  3. Laboratory of the Marine Biotoxins, National institute of Halieutic Research, Casablanca, Morocco

    HAMID TALEB

Authors
  1. NADIA TAKATI
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  2. DRISS MOUNTASSIF
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  3. HAMID TALEB
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  4. MOHAMED BLAGHEN
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Editor information

Editors and Affiliations

  1. Bulgarian Academy of Sciences, Sofia, Bulgaria

    Lubomir Simeonov

  2. Ovidius State University, Constanta, Romania

    Elisabeta Chirila

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TAKATI, N., MOUNTASSIF, D., TALEB, H., BLAGHEN, M. (2006). DETERMINATION OF PROTEIN COMPLEXED PARALYTIC SHELLFISH POISONING. MECHANISM OF THE REMEDIATION (DETOXIFICATION) OF CHEMICALS (PESTICIDE, HEAVY METALS, OTHER TOXIC CHEMICAL COMPOUNDS). In: Simeonov, L., Chirila, E. (eds) Chemicals as Intentional and Accidental Global Environmental Threats. NATO Security through Science Series. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5098-5_24

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