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Horner, M.; McComb, J.A.; McComb, A.J. and Street, H.E. (1977) Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J. Exp. Bot. 28: 13651372.
Wetzstein, H.Y.; Kim, C. and Sommer, H.E. (1994) Vessel volume, gelling agent, and basal salts affect pH and gel strength of autoclaved tissue culture media. Hort Sci. 29:683-685.
Huang, L.-C.; Kohashi, C.; Vangundy, R. and Murashige, T. (1995) Effects of common components on hardness of culture media prepared with Gelriteâ„¢. In Vitro Cell Dev. Biol.-Plant 31: 84-89.
Klimaszewska, K. and Smith, D.R. (1997) Maturation of somatic embryos of Pinus strobusis promoted by a high concentration of gellan gum. Physiol. Plant. 100: 949-957.
Romberger, J.A. and Tabor, C.A. (1971) The Picea abies shoot apical meristem in culture. I. Agar and autoclaving effects. Amer. J. Bot. 58: 131-140.
Brown, D.C.W.; Leung, D.W.M. and Thorpe, T.A. (1979) Osmotic requirement for shoot formation in tobacco callus. Physiol. Plant. 46: 36-41.
von Arnold, S. (1987) Improved efficiency of somatic embryogenesis in mature embryos of Picea abies(L.) Karst. J.Plant Physiol. 128: 233-244.
Li, X.Y.; Huang, F.H. and Gbur, E.E., Jr. (1998) Effect of basal medium, growth regulators and Phytagel concentration on initiation of embryogenic cultures from immature zygotic embryos of loblolly pine (Pinus taeda L.) Plant Cell Rep.17: 298-301.
Bornmann, C.H. and Vogelmann, T.C. (1984) Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in vitro in Pice abies. Physiol. Plant. 61: 505-512.
Klimaszewska, K.; Bernier-Cardou, M.; Cyr, D.R. and Sutton, B.C.S. (2000) Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L. In Vitro Cell. Dev. Biol.–Plant 36: 279-286.
Pasqualetto, P.-L.; Zimmerman, R.H. and Fordham, I. (1988) The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tissue Org. Cult. 14:31-40.
Nairn, B.J.; Furneaux, R.H. and Stevenson, T.T. (1995) Identification of an agar constituent responsible for hydric control in micropropagation of radiata pine. Plant Cell Tissue Org. Cult. 43: 1-11.
Marga, F.; Vebret, L. and Morvan, H. (1997) Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity. Plant Cell Tissue Org. Cult. 49: 1-5.
Owens, L.D. and Wozniak, C.A. (1991) Measurement and effects of gel matric potential ad expressibility on production of morphogenic callus by cultured sugarbeet leaf discs. Plant Cell Tissue Org. Cult. 26: 127-133.
Singha, S.; Townsend, E.C. and Oberly, G.H. (1985) Mineral nutrient status of crabapple and pear shoots cultured in vitro on varying concentrations of three agars. Amer. J. Hort. Sci. 110: 407-411.
Stecchini, M.L.; Del Torre, M.; Sarais, I.; Saro, O.; Messina, M. and Maltini, E. (1998) Influence of structural properties and kinetic constraints on Bacillus cereus growth. Appl. Environ. Microbiol. 64: 1075-1078.
Debergh, P.C. (1982) Physical properties of culture media. In: Fujiwara, A. (Ed.) Proc. of the 5thInternational Congress of Plant Tissue and Cell Culture. Tokyo, Japan; pp. 135-136.
Debergh, P.C.; Harbaoui, Y. and Lemeur, R. (1981) Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol. Plant. 53: 81-87.
Spomer, L.A. and Smith, M.A.L. (1996) Direct measurement of water availability in gelled plant tissue culture media. In Vitro Cell. Dev. Biol. - Plant 32: 210-215.
Debergh, P.C. (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant. 59: 270-276.
Beruto, D.; Beruto, M.; Ciccarelli, C. and Debergh, P. (1995) Matric potential evaluations and measurements for gelled substrates. Physiol. Plant. 94: 151-157.
Whyte, J.N.C.; Englar, J.R. and Hosford, S.P.C. (1984) Factors affecting texture profile evaluation of agar gels. Botanica Marina 27: 63-69.
Cameron, S.I. (2001) Use of a prototype gel tester to demonstrate the effect of variable calcium concentration on gel rigidity. In Vitro Cell. Dev. Biol.- Plant 37: 419-424.
Mao, R.; Tang, J. and Swanson, B.G. (2001) Water holding capacity and microstructure of gellan gels. Carbohydrate Polymers 46: 365-371.
Sarma, K.S., Maesato, K.; Hara, T. and Sonoda, T. (1990) Effect of method of agar addition on post-autoclave pH of the tissue culture media. Ann. Bot. 65: 37-40.
Owen, H.R., Wengerd, D., and Miller, A.R. (1991) Culture medium is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method. Plant Cell Rep.10: 583-586.
Skirvin, R.M.; Chu, M.C.; Mann, M.L.; Young, H.; Sullivan, J. and Fermanian, T. (1986) Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material. Plant Cell Rep. 5: 292-294.
Selby, C., Lee, R., and Harvey, B.M.R. (1989) The effects of culture medium rigidity on adventitious bud production and tissue vitrification in needle cultures of Sitka spruce [Picea sitchensis(Bong.) Carr.] New Phytol. 113: 203-210.
McNaught, A.D. and Wilkinson, A. (1997) IUPAC Compendium of Chemical Technology 2nd ed. Blackwell Science, Oxford, UK; pp. 464. Online at: http://www.nicmila.org/ Gold/Output/S06227.xhtml.
Krieg, N.R. and Gerhardt, P. (1981) Solid culture. In: Gerhardt, P.; Murray, R.G.E.; Costilow, R.N.; Nester, E.W.; Wood, W.A.; Krieg, N.R. and Phillips, G.B. (Eds.) Manual of Methods for General Bacteriology. Amer. Soc. For Microbiol., Washington DC; pp. 143-150.
Scherer, P.A.; Muller, E.; Lippert, H. and Wolff, G. (1988) Multi-element analysis of agar and gelrite impurities investigated by inductively coupled plasma emission spectrometry as well as physical properties of tissue culture media prepared with agar or the gellan gum Gelrite. Acta Hort. 226: 655-658.
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Cameron, S.I. (2008). Tissue Culture Gel Firmness: Measurement And Effects On Growth. In: Gupta, S.D., Ibaraki, Y. (eds) Plant Tissue Culture Engineering. Focus on Biotechnology, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-3694-1_17
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DOI: https://doi.org/10.1007/978-1-4020-3694-1_17
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