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Repression Of Matrix Gene Expression By B-Myb

  • Claudia S. Hofmann
  • Gail E. Sonenshein
Part of the Proteins and Cell Regulation book series (PROR, volume 2)

Vascular smooth muscle cells (SMCs) synthesise collagens type I and V matrix proteins, which are major constituents of the arterial wall. In culture, matrix gene expression varies inversely with the rate of SMC proliferation. Previously we showed that B-myb, a member of the myb gene family, is expressed in SMCs in a cell-cycle dependent fashion, and that it is a negative regulator of matrix gene transcription. Phosphorylation by cyclin A/cdk2 relieved B-Myb-mediated repression of α2 (V) collagen gene transcription, and the sites of phosphorylation were distinct from those affecting activation by B-Myb. The domain responsible for repression mapped to residues 491 to 582 of the C-terminal region of B-Myb. Transgenic mice over-expressing BMyb displayed significantly reduced collagen expression in the aorta. Thus, B-Myb functions in vivo as a repressor of collagen gene expression in vascular SMCs.

Keywords

Basic Fibroblast Growth Factor Collagen Gene Type Versus Collagen Collagen Gene Expression Vascular SMCs 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Claudia S. Hofmann
    • 1
  • Gail E. Sonenshein
    • 1
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

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