Effect of Physiological Status on Endogenous Excretion of Purine Derivatives in Cattle
The present study examined the endogenous urinary excretion of purine derivatives (PD: allantoin and uric acid) in normally fed animals in different physiological states. Animals fitted with a simple rumen cannula and a T-shaped duodenal cannula were used in three separate experiments. In Experiment I, three heifers (about 8 months old; 225 ±4.4 kg) fed a cereal based diet were used. In Experiment II, two Friesian dry cows (about 24 months old; 696 ±21 kg) fed at maintenance level on chopped barley straw and barley grain (50:50). In Experiment 3, three multiparous crossbreed Holstein-Friesian cows (560 ±10 kg, average milk yield 25 ±3.2 kg/d) in their third lactation were used. The cows were fed a mixed diet (48:52; roughage: concentrate). 15N ammonium phosphate was infused continuously into the rumen to label microbial purine bases (PB). Duodenal flow of digesta and PB was determined using a dual marker system. After 72–80 h purine enrichment had reached plateau values in body pools and in urine. Daily endogenous PD excretion (µmol/W0.75) obtained in dry cows (310 ±31.0) were not significantly different from that obtained in growing steers (236 ±6.0) but were consistently higher (512.4 ±36.4) in lactating cows.
KeywordsHydrolysis Corn Lactate Creatinine Straw
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