Effect of Physiological Status on Endogenous Excretion of Purine Derivatives in Cattle
The present study examined the endogenous urinary excretion of purine derivatives (PD: allantoin and uric acid) in normally fed animals in different physiological states. Animals fitted with a simple rumen cannula and a T-shaped duodenal cannula were used in three separate experiments. In Experiment I, three heifers (about 8 months old; 225 ±4.4 kg) fed a cereal based diet were used. In Experiment II, two Friesian dry cows (about 24 months old; 696 ±21 kg) fed at maintenance level on chopped barley straw and barley grain (50:50). In Experiment 3, three multiparous crossbreed Holstein-Friesian cows (560 ±10 kg, average milk yield 25 ±3.2 kg/d) in their third lactation were used. The cows were fed a mixed diet (48:52; roughage: concentrate). 15N ammonium phosphate was infused continuously into the rumen to label microbial purine bases (PB). Duodenal flow of digesta and PB was determined using a dual marker system. After 72–80 h purine enrichment had reached plateau values in body pools and in urine. Daily endogenous PD excretion (µmol/W0.75) obtained in dry cows (310 ±31.0) were not significantly different from that obtained in growing steers (236 ±6.0) but were consistently higher (512.4 ±36.4) in lactating cows.
KeywordsPurine Base Barley Grain Purine Derivative Intragastric Infusion Creatinine Excretion Rate
Unable to display preview. Download preview PDF.
- GIESECKE, D., STANGASSINGER, M., TIEMEYER, W., Nucleic acid digestion and urinary purines metabolites in sheep nourished by intra-gastric infusion, Can. J. Anim. Sci. 64 (1984) 144–145.Google Scholar
- FAICHNEY, G.J., “The use of markers to partition digestion within the gastrointestinal tract of ruminants”, Digestion and Metabolism in the Ruminant, (I.W. MACDONALD, A.C.I. WARNER, Eds.), University of New England Publishing Unit, Armidale, Australia (1975) pp. 277–291.Google Scholar
- MARTIN-ORUE, S.M., BALCELLS, J., GUADA, J.A., FONDEVILA, M., Microbial nitrogen production in growing heifers: direct measurement of duodenal flow of purine bases versus urinary excretion of purine derivatives as estimation procedures, Anim. Feed Sci. Technol. 88 (2000) 171–188.Google Scholar
- KERR, J.E., SERAIDARIAN, K., The separation of purine nucleosides from free purines and determination of the purines and ribose in these fractions, J. Biol. Chem. 159 (1945) 211–225.Google Scholar
- YOUNG, E.G., CONWAY, C.F., On the estimation of allantoin by the RiminiSchryver reaction, J. Biol. Chem. 142 (1942) 839–853.Google Scholar
- PÉREZ, J.F., BALCELLS, J., GUADA, J.A., CASTRILLO, C., Determination of rumen microbial-nitrogen production in sheep: a comparison of urinary purine excretion with methods using 15N and purine bases as markers of microbial-nitrogen entering the duodenum, Br. J. Nutr. 75 (1996) 699–709.PubMedCrossRefGoogle Scholar
- GONDA, H.L., EMMANUELSON, M., MURPHY, M., The effect of roughage to concentrate ratio in the diet on nitrogen and purine metabolism in dairy cows, Anim. Feed Sci. Technol. 64 (1996) 27–42.Google Scholar
- SMITH, R.H., “Nitrogen Metabolism in the rumen and the composition and nutritive value of nitrogen compounds entering the duodenum”, Digestion and Metabolism in the Ruminant, (I.W. MACDONALD, A.C.I. WARNER, Eds.), University of New England Publishing Unit, Armidale, Australia (1975) pp. 399–415.Google Scholar
- KIRCHGESSNER VON, M., KREUZER, M., Harstoff und allantoin in der Milch und Allantoinausscheidung von Kühen wäherend ung nach Verfütterung zu hoher und zu niedriges Proteinmengen. 5. Mitteilung. Zum Einflub von proteinfehlernáhrung bei laktiereden Kúben und daraus entshenden Nachwirkirkungen, J. Anim. Physiol. Anim. Nutr. 70 (1985) 82.Google Scholar
- DANIELS, Z.M., CHEN., X.B., KYLE., D.J., SINCLAIR, K., ØRSKOV, E.R., Purine derivatives in urine and plasma of lactating cows given different levels of feed intake, Anim. Prod. 58 (1994) 453 (Abst.).Google Scholar