Estimation of Microbial Protein Supply of Lactating Dairy Cows under Smallholder Farms in North-East Thailand Using Urinary Purine Derivative Technique

  • O. Pimpa
  • J. B. Liang
Conference paper


Two experiments were conducted to examine the potential of urinary purine derivative (PD) as a predictive index of microbial protein supply in ruminant livestock under farm conditions. Results of Experiment 1 indicated that diurnal variation in the PDC index ( [mmol/L PD]/[mmol/L creatinine] kgW0.75) in spot urine samples of zebu cattle was small and highly correlated with the daily PD output, suggesting that spot urine samples could be used to derive an index for estimating microbial protein supply of cattle under farm conditions. However, the PDC index for buffaloes was poorly correlated to daily urinary PD output, therefore the use of spot urine samples appeared to be unsuitable for buffaloes. Based on the above results, spot urine samples were used to estimate the microbial protein supply of lactating dairy cows under farm conditions in a follow-up experiment. The study was conducted using 24 lactating cows in 6 smallholder dairy farms situated in Khon Kaen province of Northeast Thailand. The study was conducted over two climatic seasons (raining and dry), where the animals were fed 5 kg of farm-mixed concentrate feed supplemented either with green grass (cut or grazing) or rice straw as roughage source during the raining and dry seasons, respectively. The results indicated that microbial protein supply was not significantly different and therefore, the nutritional status of the lactating cows was not significantly different between the two seasons. The absence of differences in milk yield between seasons seems to support our findings. We conclude that urinary PD technique could be used to estimate rumen microbial protein production for dairy cattle under farm conditions.


Rice Straw Milk Yield Smallholder Farm Farm Condition Spot Urine Sample 
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  1. [1]
    BALCELLS, J., GUADA, J.A., CASTRILLO, C., GASA, J., Urinary excretion of allantoin and allantoin precursors by sheep after different rate of purine infusion into the duodenum, J. Agric. Sci., Camb. 116 (1991) 309–317.CrossRefGoogle Scholar
  2. [2]
    CHEN, X.B., CHEN, Y.K., FRANKLIN, M.F., ØRSKOV, E.R., SHAND, W.J., The effect of feed intake and body weight on purine derivative excretion and microbial protein supply in sheep, J. Anim. Sci. 70 (1992) 1534–1542.PubMedGoogle Scholar
  3. [3]
    VERBIC, J., CHEN, X.B., MACLEOD, N.A., ØRSKOV, E.R., Excretion of purine derivatives by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers, J. Agric. Sci. Camb. 114 (1990) 243–248.CrossRefGoogle Scholar
  4. [4]
    PIMPA, O., LIANG, J.B., JELAN, Z.A., ABDULLAH, N., Urinary excretion of duodenal purine derivatives in Kedah-Kelantan cattle, Anim. Feed Sci. Technol. 92 (2001) 203–214.Google Scholar
  5. [5]
    CHEN, X.B., MEJIA, A.T., KYLE, D.J., ØRSKOV, E.R., Evaluation of the use of the purine derivative:creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants: study in sheep, J. Agric. Sci. Camb. 125 (1995) 137–143.CrossRefGoogle Scholar
  6. [6]
    CHEN, X.B., GRUBIC, G., ØRSKOV, E.R., OSUJI, P., Effect of feeding frequency on diurnal variation in plasma and urinary purine derivatives in steers, Anim. Prod. 55 (1992) 185–191.Google Scholar
  7. [7]
    PIMPA, O., LIANG, J.B., BALCELLS, J., JELAN, Z.A., ABDULLAH, N., Urinary purine derivatives excretion in Swamp buffaloes after purine bases infusion into the duodenum, (paper submitted to Animal Feed Science and Technology) (2002).Google Scholar
  8. [8]
    BALCELLS, J., GUADA, J.A., PEIRO, J.M., PARKER, D.S., Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography, J. Chromatography 575 (1992) 153–157.CrossRefGoogle Scholar
  9. [9]
    PIMPA, O., Urinary purine derivatives excretion as a method for estimation of rumen microbial protein production in swamp buffaloes and Zebu cattle, PhD Thesis, Universiti Putra Malaysia, Malaysia (2002).Google Scholar
  10. [10]
    VAGNONI, D.B., BRODERICK, G.A., CLAYTON, M.K., HATFIELD, R.D., Excretion of purine derivatives by Holstein cows abomasally infused with incremental amounts of purines, J. Dairy Sci. 80 (1997) 1695–1702.PubMedCrossRefGoogle Scholar
  11. [11]
    SAS, Statistical Analysis Systems Institute, SAS® User’s Guide statistic, Version 6.03, Cary, NC (1988).Google Scholar
  12. [12]
    VERCOE, J.E., Urinary allantoin excretion and digestible dry-matter intake in cattle and buffalo, J. Agric. Sci. Camb. 86 (1976) 613–615.CrossRefGoogle Scholar
  13. [13]
    LIANG, J.B., MATSUMOTO, M., YOUNG, B.A., Purine derivative excretion and ruminal microbial yield in Malaysian cattle and swamp buffalo, Anim. Feed Sci. Technol. 47 (1994) 189–199.Google Scholar
  14. [14]
    AGRICULTURAL RESEARCH COUNCIL, The Nutrient Requirements of Ruminant Livestock, Supplement No. 1, Commonwealth Agricultural Bureau, Slough, Farnham Royal, UK (1984).Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2004

Authors and Affiliations

  • O. Pimpa
    • 1
  • J. B. Liang
    • 2
  1. 1.Faculty of Agriculture Environment and Natural ResourcesNaresuan UniversityPitsanulokThailand
  2. 2.Department of Animal ScienceUniversiti Putra MalaysiaSerdangMalaysia

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