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Cytokinin Biosynthesis and Metabolism

  • Hitoshi Sakakibara

Abstract

Since the discovery of cytokinins in the 1950s, it has been clearly established that they play an important role in various processes in the growth and development of plants, including the promotion of cell division, the counteraction of senescence, the regulation of apical dominance and the transmission of nutritional signals. Kinetin (Fig. 1a) was the first substance to be identified as a cytokinin, and although it was isolated from autoclaved herring sperm DNA (34) it is not naturally produced and has not been found in living plants. The naturally occurring cytokinin trans-zeatin (tZ, Fig. 1b) was first isolated from immature maize endosperm in the early 1960s (26). In the following 40 years, several cytokinin species have been identified from various plant species (35, 43). These studies demonstrated that natural plant cytokinins are adenines which have substituted at the N6 terminal either an isoprene-derived side chain (isoprenoid cytokinins), or an aromatic derivative side chain (aromatic cytokinins).

Keywords

Purine Nucleoside Phosphorylase Cytokinin Oxidase Cytokinin Biosynthesis Adenine Phosphoribosyltransferase Cytokinin Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Akiyoshi DE, Klee H, Amasino RM, Nester EW, Gordon MP. (1984) T-DNA of Agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. Proc Natl Acad Sci USA 81: 5994-5998CrossRefPubMedGoogle Scholar
  2. 2.
    Allen M, Qin W, Moreau F, Moffatt B. (2002) Adenine phosphoribosyltransferase isoforms of Arabidopsis and their potential contributions to adenine and cytokinin metabolism. Physiol Plant 115: 56-68CrossRefPubMedGoogle Scholar
  3. 3.
    Åstot C, Dolezal K, Nordström A, Wang Q, Kunkel T, Moritz T, Chua NH, Sandberg G. (2000) An alternative cytokinin biosynthesis pathway. Proc Natl Acad Sci USA 97: 14778-14783CrossRefPubMedGoogle Scholar
  4. 4.
    Barry GF, Rogers SG, Fraley RT, Brand L. (1984) Identification of a cloned cytokinin biosynthetic gene. Proc Natl Acad Sci USA 81: 4776-4780CrossRefPubMedGoogle Scholar
  5. 5.
    Bassil NV, Mok D, Mok MC. (1993) Partial purification of a cis-trans-isomerase of zeatin from immature seed of Phaseolus vulgaris L. Plant Physiol 102: 867-872PubMedGoogle Scholar
  6. 6.
    Bernier G, Havelange A, Houssa C, Petitjean A, Lejeune P. (1993) Physiological signals that induce flowering. Plant Cell 5: 1147-1155CrossRefPubMedGoogle Scholar
  7. 7.
    Bilyeu KD, Cole JL, Laskey JG, Riekhof WR, Esparza TJ, Kramer MD, Morris RO. (2001) Molecular and biochemical characterization of a cytokinin oxidase from maize. Plant Physiol 125: 378-386CrossRefPubMedGoogle Scholar
  8. 8.
    Blackwell JR, Horgan R. (1993) Cloned Agrobacterium tumefaciens ipt1 gene product, DMAPP:AMP isopentenyl transferase. Phytochemistry 34: 1477-1481CrossRefGoogle Scholar
  9. 9.
    Brandon DL, Corse J, Higaki PC, Zavala ME. (1992) Monoclonal antibodies for analysis of cytokinin-O-glucosides in response to cold stress. In Kamínek M, Mok DWS, Zazímalová E, eds, Physiology and Biochemistry of Cytokinins in Plants. SPB Academic Publishing, The Hague, pp447-453Google Scholar
  10. 10.
    Chatfield JM, Armstrong DJ. (1986) Regulation of cytokinin oxidase activity in callus tissues of Phaseolus vulgaris L. cv Great Northern. Plant Physiol 80: 493-499CrossRefPubMedGoogle Scholar
  11. 11.
    Chen C-M, Eckert RL. (1977) Phosphorylation of cytokinin by adenosine kinase from wheat germ. Plant Physiol. 59: 443CrossRefPubMedGoogle Scholar
  12. 12.
    Chen C-M, Kristopeit SM. (1981) Metabolism of cytokinin: Dephosphorylation of cytokinin ribonucleotide by 5'-nucleotidases from wheat germ cytosol. Plant Physiol 67: 494-498CrossRefPubMedGoogle Scholar
  13. 13.
    Chen C-M, Leisner SM. (1984) Modification of cytokinins by cauliflower microsomal enzymes. Plant Physiol. 75: 442-446CrossRefPubMedGoogle Scholar
  14. 14.
    Chen C-M, Petschow B. (1978) Metabolism of cytokinin: Ribosylation of cytokinin bases by adenosine phosphorylase from wheat germ. Plant Physiol 62: 871-874CrossRefPubMedGoogle Scholar
  15. 15.
    Emery RJN, Leport L, Barton JE, Turner NC, Atkins A. (1998) cis-Isomers of cytokinins predominate in chickpea seeds throughout their development. Plant Physiol 117: 1515-1523CrossRefPubMedGoogle Scholar
  16. 16.
    Entsch B, Parker CW, Letham DS. (1983) An enzyme from lupin seeds forming alanine derivatives of cytokinins. Phytochemistry 22: 375-381CrossRefGoogle Scholar
  17. 17.
    Entsch B, Parker CW, Letham DS, Summons RE. (1979) Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins. Biochim Biophys Acta 570: 124-139PubMedGoogle Scholar
  18. 18.
    Faiss M, Zalubilova J, Strnad M, Schmulling T. (1997) Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants. Plant J 12: 401-415CrossRefPubMedGoogle Scholar
  19. 19.
    Golovko A, Sitbon F, Tillberg E, Nicander B. (2002) Identification of a tRNA isopentenyltransferase gene from Arabidopsis thaliana. Plant Mol Biol 49: 161-169CrossRefPubMedGoogle Scholar
  20. 20.
    Houba-Herin N, Pethe C, d'Alayer J, Laloue M. (1999) Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts. Plant J 17: 615-626CrossRefPubMedGoogle Scholar
  21. 21.
    Ihara M, Taya Y, Nishimura S, Tanaka Y. (1984) Purification and some properties of delta 2- isopentenylpyrophosphate:5'AMP delta 2-isopentenyltransferase from the cellular slime mold Dictyostelium discoideum. Arch Biochem Biophys 230: 652-660CrossRefPubMedGoogle Scholar
  22. 22.
    Kakimoto T. (2001) Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases. Plant Cell Physiol 42: 677-685CrossRefPubMedGoogle Scholar
  23. 23.
    Klämbt D. (1992) The biosynthesis of cytokinins in higher plants: our present knowledge. In Kamínek M, Mok DWS, Zazímalová E, eds, Physiology and Biochemistry of Cytokinins in Plants. SPB Academic Publishing, The Hague, pp25-27Google Scholar
  24. 24.
    Krall L, Raschke M, Zenk MH, Baron C. (2002) The Tzs protein from Agrobacterium tumefaciens C58 produces zeatin riboside 5'-phosphate from 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate and AMP. FEBS Lett 527: 315-318CrossRefPubMedGoogle Scholar
  25. 25.
    Laureys F, Dewitte W, Witters E, Van Montagu M, Inze D, Van Onckelen H. (1998) Zeatin is indispensable for the G2-M transition in tobacco BY-2 cells. FEBS Lett 426: 29-32CrossRefPubMedGoogle Scholar
  26. 26.
    Letham DS. (1963) Zeatin, a factor inducing cell division from Zea mays. Life Sci 8: 569-573CrossRefPubMedGoogle Scholar
  27. 27.
    Letham DS. (1994) Cytokinins as phytohormones-sites of biosynthesis, translocation, and function of translocated cytokinin. In Mok DWS, Mok MC, eds, Cytokinins: Chemistry, Activity, and Function. CRC Press, Boca Raton, Florida, pp.57-80Google Scholar
  28. 28.
    Letham DS, Palni LMS, Tao G-Q, Gollnow BI, Bates CM. (1983) Regulators of cell division in plant tissues XXIX. The activities of cytokinin glucosides and alanine conjugates in cytokinin bioassays. J. Plant Growth Regul. 2: 103-115CrossRefGoogle Scholar
  29. 29.
    Martin RC, Mok MC, Habben JE, Mok DW. (2001) A maize cytokinin gene encoding an O-glucosyltransferase specific to cis-zeatin. Proc. Natl. Acad. Sci. USA 98: 5922-5926CrossRefPubMedGoogle Scholar
  30. 30.
    Martin RC, Mok MC, Mok DW. (1999) A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris. Plant Physiol. 120: 553-558CrossRefPubMedGoogle Scholar
  31. 31.
    Martin RC, Mok MC, Mok DW. (1999) Isolation of a cytokinin gene, ZOG1, encoding zeatin O-glucosyltransferase from Phaseolus lunatus. Proc. Natl. Acad. Sci. USA 96: 284-289CrossRefPubMedGoogle Scholar
  32. 32.
    Martin RC, Mok MC, Shaw G, Mok DWS. (1989) An enzyme mediating the conversion of zeatin to dihydrozeatin in Phaseolus embryos. Plant Physiol 90: 1630-1635CrossRefPubMedGoogle Scholar
  33. 33.
    McGaw BA, Horgan R. (1983) Cytokinin oxidase from Zea mays kernels and Vinca rosea crown-gall tissue. Planta 159: 30-37CrossRefGoogle Scholar
  34. 34.
    Miller CO, Skoog F, Saltza vNH, Strong M. (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J. Am. Chem. Soc. 77: 1329-1334Google Scholar
  35. 35.
    Mok DW, Mok MC. (2001) Cytokinin Metabolism and Action. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52: 89-118CrossRefPubMedGoogle Scholar
  36. 36.
    Mok DWS, Martin RC. (1994) Cytokinin metabolic enzymes. In Mok DWS, Mok MC, eds, Cytokinins: Chemistry, Activity, and Function. CRC Press, Boca Raton, Florida, pp.129-137Google Scholar
  37. 37.
    Morris RO, Bilyeu KD, Laskey JG, Cheikh NN. (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem Biophys Res Commun 255: 328-333CrossRefPubMedGoogle Scholar
  38. 38.
    Murai N. (1994) Cytokinin biosynthesis in tRNA and cytokinin incorporation into plant RNA. In Mok DWS, Mok MC, eds, Cytokinins: Chemistry, Activity, and Function. CRC Press, Boca Raton, Florida, pp.87-99Google Scholar
  39. 39.
    Schnorr KM, Gaillard C, Biget E, Nygaard P, Laloue M. (1996) A second form of adenine phosphorybosyltransferase in Arabidopsis thaliana with relative specificity towards cytokinins. Plant J. 9: 891-898CrossRefPubMedGoogle Scholar
  40. 40.
    Shimizu-Sato S, Mori H. (2001) Control of outgrowth and dormancy in axillary buds. Plant Physiol 127: 1405-1413CrossRefPubMedGoogle Scholar
  41. 41.
    Skoog F, Armstrong DJ. (1970) Cytokinins. Ann. Rev. Plant Physiol. 21: 359-384CrossRefGoogle Scholar
  42. 42.
    Spiess LD. (1975) Comparative activity of isomers of zeatin and ribosyl-zeatin on Funaria hygrometrica. Plant Physiol 55: 583-585CrossRefPubMedGoogle Scholar
  43. 43.
    Strnad M. (1997) The aromatic cytokinins. Physiol Plant 101: 674-688CrossRefGoogle Scholar
  44. 44.
    Suttle JC, Banowetz GM. (2000) Changes in cis-zeatin and cis-zeatin riboside levels and biological activity during tuber dormancy. Physiol Plant 101: 68-74CrossRefGoogle Scholar
  45. 45.
    Takei K, Dekishima Y, Eguchi T, Yamaya T, Sakakibara H. (2003) A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling. J Plant Res. 116: 259-263CrossRefPubMedGoogle Scholar
  46. 46.
    Takei K, Sakakibara H, Sugiyama T. (2001) Identification of genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, in Arabidopsis thaliana. J. Biol. Chem. 276: 26405-26410CrossRefPubMedGoogle Scholar
  47. 47.
    Takei K, Sakakibara H, Taniguchi M, Sugiyama T. (2001) Nitrogen-dependent accumulation of cytokinins in root and the translocation to leaf: implication of cytokinin species that induces gene expression of maize response regulator. Plant Cell Physiol. 42: 85-93CrossRefPubMedGoogle Scholar
  48. 48.
    Takei K, Ueda N, Aoki K, Kuromori T, Hirayama T, Shinozaki K, Yamaya T, Sakakibara H. (2004) AtIPT3, an Arabidopsis isopentenyltransferase gene, is a key determinant of macronutrient-responsive cytokinin biosynthesis. Plant Cell physiol. In press.Google Scholar
  49. 49.
    Tarkowska D, Dolezal K, Tarkowski P, Åstot C, Holub J, Fuksova K, Schmülling T, Sandberg G, Strnad M. (2003) Identification of new aromatic cytokinins in Arabidopsis thaliana and Populus x canadensis leaves by LC-(+)ESI-MS and capillary liquid chromatography/frit-fast atom bombardment mass spectrometry. Physiol Plant 117: 579-590CrossRefPubMedGoogle Scholar
  50. 50.
    Taya Y, Tanaka Y, Nishimura S. (1978) 5'-AMP is a direct precursor of cytokinin in Dictyostelium discoideum. Nature 271: 545-547CrossRefPubMedGoogle Scholar
  51. 51.
    Veach YK, Martin RC, Mok DW, Malbeck J, Vankova R, Mok MC. (2003) O-glucosylation of cis-zeatin in maize. Characterization of genes, enzymes, and endogenous cytokinins. Plant Physiol 131: 1374-1380CrossRefPubMedGoogle Scholar
  52. 52.
    von Schwartzenberg K, Kruse S, Reski R, Moffatt B, Laloue M. (1998) Cloning and characterization of an adenosine kinase from Physcomitrella involved in cytokinin metabolism. Plant J 13: 249-257CrossRefGoogle Scholar
  53. 53.
    Yamada H, Suzuki T, Terada K, Takei K, Ishikawa K, et al. (2001) The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. Plant Cell Physiol 42: 1017-1023CrossRefPubMedGoogle Scholar
  54. 54.
    Yonekura-Sakakibara K, Yamaya T, Sakakibara H. (2004) Molecular characterization of cytokinin-responsive histidine kinases in maize: differential ligand preference and response to cis-zeatin. Plant Physiol. 134: (4) 1654-1661CrossRefPubMedGoogle Scholar
  55. 55.
    Zubko E, Adams CJ, Machaekova I, Malbeck J, Scollan C, Meyer P. (2002) Activation tagging identifies a gene from Petunia hybrida responsible for the production of active cytokinins in plants. Plant J 29: 797-808CrossRefPubMedGoogle Scholar
  56. 56.
    Chen CM, Kristopeit SM. (1981) Metabolism of cytokinins: deribosylation of cytokinin ribonucleoside by adenosine nucleosidase from wheat germ cells. Plant Physiol 68: 1020-1023CrossRefPubMedGoogle Scholar
  57. 57.
    Galuszka P, Frébort I, Sebela M, Sauer P, Jacobsen S, Pec P. (2001) Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin degradation in cereals. Eur J Biochem 268: 450-461CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Plant Science CenterTsurumiJapan

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