Attenuation Mapping of Living Cells at Hypersonic Frequencies

  • Chiaki Miyasaka
  • Bernhard R. Tittmann
Conference paper
Part of the Acoustical Imaging book series (ACIM, volume 27)


A mechanical scanning acoustic reflection microscope (SAM) with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the SAM. The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g. shrinkage, volume change or lift-off) were clearly visualized.


Melanoma Cell Sapphire Substrate Attenuation Mapping Heating Plate Curve Simulation 
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Copyright information

© Springer Science+Business Media Dordrecht 2004

Authors and Affiliations

  • Chiaki Miyasaka
    • 1
  • Bernhard R. Tittmann
    • 1
  1. 1.The Pennsylvania State UniversityUniversity ParkUSA

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