Attenuation Mapping of Living Cells at Hypersonic Frequencies
A mechanical scanning acoustic reflection microscope (SAM) with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the SAM. The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g. shrinkage, volume change or lift-off) were clearly visualized.
KeywordsMelanoma Cell Sapphire Substrate Attenuation Mapping Heating Plate Curve Simulation
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- 2.R. A. Lemons and C. F. Quate, Acoustic microscopy. Physical Acoustics XIV (ed by W. P. Mason and R. N. Thurston), (1979), p.1, Academic Press, LondonGoogle Scholar
- 4.K. Beck and J. Bereiter-Hahn, “Evaluation of reflection interference contrast microscopy images of living cells,” Micros. Acta 84 (1981), p.153Google Scholar
- 6.J. Bereiter-Hahn and H. Lüers, “Shape changes and force distribution in locomoting cells. Investigation with reflected light and acoustic microscopy,” Eur. J. Cell Biol. 53: Suppl. 31, (1990), p.85Google Scholar
- 7.H. Lüers, K. Hillmann, J. Litniewski, and J. Bereiter-Hahn, “Acoustic microscopy of cultured cell: distribution of forces and cytokeletal elements,” Cell Biophys. 18, (1992), p.279Google Scholar
- 10.J. Bereiter-Hahn, R. Strohmeier, and K. Beck, “Determination of the thickness profile of cells with the reflection contrast microscope,” Scient. Techn. Inf 8 (1983), p.125Google Scholar
- 11.R. Strohmeier and J. Bereiter-Hahn, “Hydrostatic pressure in epidermal cells is dependent on Camediated contraction,” J. Cell Sci. 88 (1987), p.631Google Scholar
- 16.N. Akashi, J. Kushibiki, and N. Chubachi, “Quantitative characterization of biological tissues by acoustic microscopy-effect of multiple reflection and viscosity,” Technical Report of the IECE 43.80 Ev. 43, (1989), p.767Google Scholar
- 17.J. Wang, R. Gundle, and G. A. D. Briggs, “The measurement of acoustic properties of living human cells,” Trans. Roy. Microsc. Soc. Vol. 1 (ed. By H. Y. Elder)(1990), p.91Google Scholar
- 18.G. A. D. Briggs, J. Wang, and R. Gundle, “Quantitative acoustic microscopy of individual living human cells,” J. Microsc. 91, (1992), p.3008Google Scholar
- 23.W. Parmon and H. L. Bertoni, “Ray interpretation of the material signature in acoustic microscope”, Electron. Lett. 15(21), (1979), p.684Google Scholar