Abstract
A mechanical scanning acoustic reflection microscope (SAM) with frequency at 1.0 GHz was used for imaging a living human skin cancer cell when applying the thermal insult to it. First, for finding the heat effect of the cell, we located a chamber having the heating plate with temperature controller on the X-Y stage of the SAM. The cell was grown on the surface of the sapphire substrate to maximize the difference in attenuation for visualizing the cells with frequency at 1.0 GHz. We gradually increased the temperature of the culturing medium from 37.5°C to 50°C, and carried out in-situ observation. The phenomena indicating cellular insult and injury (e.g. shrinkage, volume change or lift-off) were clearly visualized.
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References
A. Atalar, C. F. Quate, and H. K. Wickramasinge, “Phase imaging in reflection with acoustic microscope,” Appl. Phys. Lett., 31 (1977), p.791
R. A. Lemons and C. F. Quate, Acoustic microscopy. Physical Acoustics XIV (ed by W. P. Mason and R. N. Thurston), (1979), p.1, Academic Press, London
J. A. Hildebrand, D. Rugar, R. N. Johnston, and C. F. Quate, “Acoustic microscopy of living cells,” Proc. National Academy of Science 78(3), (1981), p.1656
K. Beck and J. Bereiter-Hahn, “Evaluation of reflection interference contrast microscopy images of living cells,” Micros. Acta 84 (1981), p.153
J. Bereiter-Hahn, “Scanning acoustic microscopy visualizes cytomechanical responses to cytochalasin D,” J. Microsc. 146 (1986), p.29
J. Bereiter-Hahn and H. Lüers, “Shape changes and force distribution in locomoting cells. Investigation with reflected light and acoustic microscopy,” Eur. J. Cell Biol. 53: Suppl. 31, (1990), p.85
H. Lüers, K. Hillmann, J. Litniewski, and J. Bereiter-Hahn, “Acoustic microscopy of cultured cell: distribution of forces and cytokeletal elements,” Cell Biophys. 18, (1992), p.279
J. Bereiter-Hahn and H. Lüers, In “Mechanics of Actively Locomoting Cells,” (N. Akkas, ed.) ASI series 84 Springer, Heidelberg, New York, Berlin, (1994), p.181
J. Bereiter-Hahn, J. Litniewski, K. Hillmann, A. Krapohl, and L. Zylberberg, “What can scanning acoustic microscopy tell about animal cells and tissues?” Acoust. Imaging 17 (1992), p.27
J. Bereiter-Hahn, R. Strohmeier, and K. Beck, “Determination of the thickness profile of cells with the reflection contrast microscope,” Scient. Techn. Inf 8 (1983), p.125
R. Strohmeier and J. Bereiter-Hahn, “Hydrostatic pressure in epidermal cells is dependent on Camediated contraction,” J. Cell Sci. 88 (1987), p.631
J. A. Hildebrand and D. Rugar, “Measurement of cellular elastic properties by acoustic microscopy,” J. Microsc. 134 (1990), p.245
J. Litniewski and J. Bereiter-Hahn, “Measurement of cells in culture by scanning acoustic microscopy,” J. Microsc. 158 (1990), p.95
C. M. W. Daft and G. A. D. Briggs, “The elastic microstructure of various tissues,” J. Acoust. Soc. Am. 85 (1989), p.416
C. M. W. Daft, G. A. D. Briggs, and W. D. O’Brien, “Frequency dependence of tissue attenuation by acoustic microscopy,” J. Acoust. Soc. Am. 85 (1989), p.2194
N. Akashi, J. Kushibiki, and N. Chubachi, “Quantitative characterization of biological tissues by acoustic microscopy-effect of multiple reflection and viscosity,” Technical Report of the IECE 43.80 Ev. 43, (1989), p.767
J. Wang, R. Gundle, and G. A. D. Briggs, “The measurement of acoustic properties of living human cells,” Trans. Roy. Microsc. Soc. Vol. 1 (ed. By H. Y. Elder)(1990), p.91
G. A. D. Briggs, J. Wang, and R. Gundle, “Quantitative acoustic microscopy of individual living human cells,” J. Microsc. 91, (1992), p.3008
T. Kundu, J. Bereiter-Hahn, and K. Hillmann, “Calculating acoustical properties of cells: influence of surface topography and liquid layer between cell and substrate,” J. Acoust. Soc. Am. 85, (1989), p.2194
A. Atalar, “An angular-spectrum approach to contrast in reflection acoustic microscopy”, J Appl. Phys. 49(10) (1978), p.5130
R. D. Weglein, “A model for predicting acoustic material signatures”, Appl. Phys. Lett. 34(3) (1979), p.179
A. Atalar, “A physical model for acoustic signature”, J. Appl. Phys. 50(12), (1979), p.8237
W. Parmon and H. L. Bertoni, “Ray interpretation of the material signature in acoustic microscope”, Electron. Lett. 15(21), (1979), p.684
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Miyasaka, C., Tittmann, B.R. (2004). Attenuation Mapping of Living Cells at Hypersonic Frequencies. In: Arnold, W., Hirsekorn, S. (eds) Acoustical Imaging. Acoustical Imaging, vol 27. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2402-3_73
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DOI: https://doi.org/10.1007/978-1-4020-2402-3_73
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