Sugarcane Transformation

  • S. J. Snyman

Abstract

Sugarcane is a hybrid (Saccharum species hybrids) belonging to the grass family, Gramineae. It is best grown commercially in tropical and sub-tropical areas that are characterized by warm temperatures, high incident solar radiation and annual rainfall, and deep fertile soils (1). Sugarcane is cultivated in over 100 countries and is the source of approx. 70% of the world’s sugar (2). In addition, it is used as a raw material for ethanol production in Brazil, which is the largest producer of cane sugar in the world (3).

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Barnes AC (1974). The sugarcane, 2nd edn. Leonard Hill Books, London UK. pp. 572.Google Scholar
  2. 2.
    Anonymous (2000). South African Sugar Association industry directory. Published by SASA Public Affairs Division.Google Scholar
  3. 3.
    http://apps.fao.org.Google Scholar
  4. 4.
    Heinz DJ (1987). Sugarcane Improvement Through Breeding. Elsevier, The Netherlands, pp.1–6.Google Scholar
  5. 5.
    Garside AL (1997). Yield decline research in the Australian sugar industry. Proceedings of the South African Sugar Technology Association, 71: 3–8.Google Scholar
  6. 6.
    Thomas JC, Adams DG, Keppenne VD, Wasmann CC, Brown JK, Kanost MR and Bohnert HJ (1995a). Manduca sexta protease inhibitors expressed in Nicotiana tabacum provide protection against insects. Plant Physiology and Biochemistry, 33: 611–614.Google Scholar
  7. 7.
    Thomas JC, Adams DG, Keppenne VD, Wasmann CC, Brown JK, Kanost MR and Bohnert HJ (1995b). Protease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports, 14: 758–762.CrossRefGoogle Scholar
  8. 8.
    Daniels J and Roach BT (1987). Taxonomy and Evolution. In: Heinz DJ (ed.), Developments in Crop Science II. Sugarcane improvement through breeding. Elsevier, Amsterdam, pp. 7–84.Google Scholar
  9. 9.
    Stevenson GC (1965). Genetics and breeding of sugarcane. Longmans, London, pp. 284.Google Scholar
  10. 10.
    Berding N and Roach RT (1987). Germplasm collection, maintenance and use. In: Heinz D (ed.), Developments in Crop Science III. Sugarcane Improvement Through Breeding. Elsevier, The Netherlands, pp.143–210.Google Scholar
  11. 11.
    Butterfield MK, D’Hont AD and Berding N (2001). The sugarcane genome: a synthesis of current understanding, and lessons for breeding and biotechnology. Proceedings of the South African Sugar Technology Association, 75: 1–5.Google Scholar
  12. 12.
    Arceneaux G (1965). Cultivated sugarcanes of the world and their botanical derivatives. Proceedings of the International Society of Sugar Cane Technology, 12: 844–855.Google Scholar
  13. 13.
    Bremer G (1961). Problems in breeding and cytology of sugarcane. IV The origin of the increase of chromosome number in species hybrids Saccharum. Euphytica, 10: 325–342.CrossRefGoogle Scholar
  14. 14.
    Bennet MD and Smith JD (1976). Nuclear DNA amounts in Angiosperms. Philosophical Transactions of the Royal Society of London Series B, 274: 227–274.CrossRefGoogle Scholar
  15. 15.
    Price S (1967). Interspecific hybridization in sugarcane breeding. Proceedings of the International Society of Sugar Cane Technology, 12: 1021–1026.Google Scholar
  16. 16.
    Dunwell JM (1999). Transgenic crops: the next generation, or an example of 2020 vision. Annals of Botany, 84: 269–277.CrossRefGoogle Scholar
  17. 17.
    Arencibia A, Vazquez RI, Prieto D, Tellez P, Carmona ER, Coego A, Hernandez L, de la Riva GA et al. (1997). Transgenic sugarcane plants resistant to stem borer attack. Molecular Breeding, 3: 247–255.CrossRefGoogle Scholar
  18. 18.
    Allsopp PG, McGhie TK, Hickman KA and Smith GR (1996). Increasing the resistance of sugarcane to attack from whitegrubs by introducing novel insecticidal genes. In: JR Wilson, DM Hogarth, JA Campbell, AL Garside (eds.), Sugarcane: Research Towards Efficient and Sustainable Production. Sugar 2000 Symposium, CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia, pp. 141–143.Google Scholar
  19. 19.
    Nutt KA, Allsopp PG, McGhie TK, Shepard KM, Joyce PA, Taylor GO, McQualter RB, Smith GR et al. (1999). Transgenic sugarcane with increased resistance to canegrubs. Proceedings of the Australian Society of Sugar Cane Technology, Townsville, Queensland, Australia, 27–30 April 1999, pp. 171–176.Google Scholar
  20. 20.
    Irvine JE and Mirkov TE (1997). The development of genetic transformation of sugarcane in Texas. Sugar Journal June1997, pp. 25–29.Google Scholar
  21. 21.
    Zhang L and Birch RG (1996). Biocontrol of sugarcane leaf scald disease by an isolate of Pantoea dispersa which detoxifies albicidin phytotoxins. Letters in Applied Microbiology, 22: 132–136.CrossRefGoogle Scholar
  22. 22.
    Joyce PA, McQualter RB, Handley JA, Dale JL, Harding RM and Smith GR (1998). Transgenic sugarcane resistant to sugarcane mosaic virus. Proceedings of the Australian Society Sugar Cane Technology, 20: 204–210.Google Scholar
  23. 23.
    Ingelbrecht IL, Irvine JE and Mirkov TE (1999). Postranscriptional gene silencing in transgenic sugarcane. Dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. Plant Physiology, 119: 1187–1197.PubMedCrossRefGoogle Scholar
  24. 24.
    Groenewald J-H and Botha FC (2001). Manipulating sucrose metabolism with a single enzyme: pyrophosphate-dependent phosphofructokinase (PFP). Proceedings of the South African Sugar Technology Association, 75: 101–103.Google Scholar
  25. 25.
    Gallo-Meagher M and Irvine JE (1996). Herbicide resistant sugarcane containing the bar gene. Crop Science, 36: 1367–1374.CrossRefGoogle Scholar
  26. 26.
    Enriquez-Obregon GA, Vazquez-Padron RI, Prieto-Samsonov DL, de la Riva GA and Selman-Housein G (1998). Herbicide resistant sugarcane plants by Agrobacterium-mediated transformation. Planta 206: 20–27.CrossRefGoogle Scholar
  27. 27.
    Falco MC, Tulmann Neto A and Ulian EC (2000). Transformation and expression of a gene for herbicide resistance in Brazilian sugarcane. Plant Cell Reports, 19: 1188–1194.CrossRefGoogle Scholar
  28. 28.
    Leibbrandt, NB and Snyman SJ (2003). Stability of gene expression and agronomic performance of a transgenic herbicide-resistant sugarcane line in South Africa. Crop Science, 43: 671–677.CrossRefGoogle Scholar
  29. 29.
    Heinz DJ and Mee GWP (1969). Plant differentiation from callus tissue of Saccharum species. Crop Science, 9: 346–348.CrossRefGoogle Scholar
  30. 30.
    Heinz DJ, Krishnamurthi M, Nickell LG and Maretzki A (1977). Cell, tissue and organ culture in sugarcane improvement. In: Reinert J, Bajaj YPS (eds.), Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Springer, Berlin, Germany, pp. 3–17.Google Scholar
  31. 31.
    Larkin PJ and Scowcroft WR (1981). Somaclonal variation- a novel source of variability from cell cultures for plant improvement. Theoretical and Applied Genetics, 60: 197–214.CrossRefGoogle Scholar
  32. 32.
    Irvine JE and Benda GTA (1985). Sugarcane mosaic virus in plantlets regenerated from diseased leaf tissue. Plant Cell, Tissue and Organ Culture, 5: 101–106.CrossRefGoogle Scholar
  33. 33.
    Lee TSG (1987). Micropropagation of sugarcane (Saccharum spp.). Plant Cell, Tissue and Organ Culture, 10: 47–55.CrossRefGoogle Scholar
  34. 34.
    Grisham MP and Bourg D (1989). Efficiency of in vitro propagation of sugarcane plants by direct regeneration from leaf tissue and by shoot-tip culture. Journal of the American Society of Sugarcane Technology, 9: 97–102.Google Scholar
  35. 35.
    Moutia M and Dookun A (1999). Evaluation of surface sterilization and hot water treatments on bacterial contaminants in bud culture of sugarcane. Experimental Agriculture, 35: 265–274.CrossRefGoogle Scholar
  36. 36.
    Bower R, Elliott AR, Potier BAM and Birch RG (1996). High-efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers. Molecular Breeding, 2: 239–249.CrossRefGoogle Scholar
  37. 37.
    Arencibia AD, Carmona ER, Tellez P, Calm M-T, Yu S-M, Trujilo LE and Oramas P (1998). An efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens. Transgenic Research, 7: 213–222.CrossRefGoogle Scholar
  38. 38.
    Arencibia AD, Carmona E, Cornide MT, Menendez E and Molina P (2000). Transgenic sugarcane (Saccharum spp.). In: Bajaj SS (ed.), Biotechnology in Agriculture and Forestry 46. Transgenic crops 1. Springer, Heidelberg, Germany, pp. 188–206.Google Scholar
  39. 39.
    Nadar HM, Soepraptopo S, Heinz DJ and Ladd SL (1978). Fine structure of sugarcane (Saccharum spp.) callus and the role of auxin in embryogenesis. Crop Science, 18: 210–216.CrossRefGoogle Scholar
  40. 40.
    Taylor PWJ, Ko H-L, Adkins SW, Rathus C and Birch RG (1992). Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell, Tissue and Organ Culture, 28: 69–78.CrossRefGoogle Scholar
  41. 41.
    Fitch MM and Moore PH (1993). Long-term culture of embryogenic sugarcane callus. Plant Cell, Tissue and Organ Culture, 32: 335–343.CrossRefGoogle Scholar
  42. 42.
    Fitch MM and Moore PH (1990). Comparison of 2,4-D and picloram for selection of long-term totipotent green callus cultures of sugarcane. Plant Cell, Tissue and Organ Culture, 20: 157–163.Google Scholar
  43. 43.
    Brisibe EA, Miyake H, Taniguchi T and Maeda E (1994). Regulation of somatic embryogenesis in long-term callus cultures of sugarcane (Saccharum officinarum L.). New Phytologist, 126: 301–307.CrossRefGoogle Scholar
  44. 44.
    Gambley RL, Ford R and Smith GR (1993). Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing β-glucuronidase. Plant Cell Reports, 12: 343–346.CrossRefGoogle Scholar
  45. 45.
    Gambley RL, Bryant JD, Masel NP and Smith GR (1994). Cytokinin-enhanced regeneration of plants from microprojectile bombarded sugarcane meristematic tissue. Australian Journal of Plant Physiology, 21: 603–612.CrossRefGoogle Scholar
  46. 46.
    Aftab F and Iqbal J (1999). Plant regeneration from protoplasts derived from cell suspension of adventive somatic embryos in sugarcane (Saccharum spp. hybrid cv. CoL-54 and cv. CP-43/33). Plant Cell, Tissue and Organ Culture, 56: 155–162.CrossRefGoogle Scholar
  47. 47.
    Snyman SJ, Watt MP, Huckett BI and Botha FC (2000). Direct somatic embryogenesis for rapid, cost effective production of transgenic sugarcane (Saccharum spp. hybrids). Proceedings of the South African Sugar Technology Association, 74: 186–187.Google Scholar
  48. 48.
    Snyman SJ, Huckett BI, Botha FC and Watt MP (2001). A comparison between direct and indirect somatic morphogenesis for the production of transgenic sugarcane (Saccharum spp. hybrids). Acta Horticulturae, 560: 105–108.Google Scholar
  49. 49.
    Elliot AR, Geijskes RJ, Lakshmanan P, McKeon MG, Wang LF, Berding N, Grof CPL and Smith GR (2002). Direct regeneration of transgenic sugarcane following microprojectile transformation of regenerable cells in thin transverse section explants. 10th International Association for Plant Tissue Culture and Biotechnology Congress, 23–28 June, Orlando, USA, poster#1376.Google Scholar
  50. 50.
    Snyman SJ, Huckett BI, Botha FC and Watt MP (2002). The use of sugarcane leaf roll discs as target material and regeneration of transgenic plants via direct embryogenesis: problems and potential. 10th International Association for Plant Tissue Culture and Biotechnology Congress, 23–28 June, Orlando, USA, poster#1460.Google Scholar
  51. 51.
    Elliot AR, Campbell JA, Brettell RIS and Grof CPL (1998). Agrobacterium-mediated transformation of sugarcane using GFP as a screenable marker. Australian Journal of Plant Physiology, 25: 739–743.CrossRefGoogle Scholar
  52. 52.
    Bower R and Birch RG (1992). Transgenic sugarcane plants via microprojectile bombardment. The Plant Journal, 2: 409–416.CrossRefGoogle Scholar
  53. 53.
    Last DI, Brettell RIS, Chamberlain DA, Chaudhury AM, Larkin PJ, Marsh EL, Peacock WJ and Dennis ES (1991). pEmu: an improved promoter for gene expression in cereal cells. Theoretical and Applied Genetics, 81: 581–588.CrossRefGoogle Scholar
  54. 54.
    Murashige T and Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473–497.CrossRefGoogle Scholar
  55. 55.
    Finer JJ, Vain P, Jones MW and McMullen MD (1992). Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Reports, 11: 323–328.CrossRefGoogle Scholar
  56. 56.
    Vain P, McMullen MD and Finer JJ (1993). Osmotic treatment enhances particle bombardment-mediated transient and stable transformation of maize. Plant Cell Reports, 12: 84–88.CrossRefGoogle Scholar
  57. 57.
    Chen WH, Davey MR, Power JB and Cocking EC (1988). Control and maintenance of plant regeneration in sugarcane callus cultures. Journal of Experimental Botany, 39: 251–261.CrossRefGoogle Scholar
  58. 58.
    Snyman SJ, Meyer GM, Carson D and Botha FC (1996). Establishment of embryogenic callus and transient gene expression in selected sugarcane varieties. South African Journal of Botany, 62: 151–154.Google Scholar
  59. 59.
    http://agnews.tamu.edu/dailynews/stories/SOIL/Apr2103a.htm.Google Scholar
  60. 60.
    http://www.bses.org.au/crcbid/Biofactory/default.htm.Google Scholar
  61. 61.
    Birch RG (1997). Plant transformation: problems and strategies for practical application. Annual Review of Plant Physiology and Plant Molecular Biology, 48: 297–326.PubMedCrossRefGoogle Scholar
  62. 62.
    Arencibia AD, Carmona ER, Cornide MT, Castiglione S, O’Relly J, Chinea A, Oramas P and Sala F (1999). Somaclonal variation in insect-resistant transgenic sugarcane (Saccharum hybrid) plants produced by cell electroporation. Transgenic Research, 8: 349–360.CrossRefGoogle Scholar
  63. 63.
    Grof C (2001). Molecular manipulation of sucrose metabolism. Proceedings of the International Society of Sugar Cane Technology, 24: 586–587.Google Scholar
  64. 64.
    Harrison D, Waldron J, Ramage C, Peace C, Cox M, Birch RG and Carroll BJ (2001). New DNA technologies provide insights into the molecular basis of somaclonal variation in sugarcane. Proceedings of the International Society of Sugar Cane Technology, 24: 580–582.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • S. J. Snyman
    • 1
  1. 1.Biotechnology DepartmentSouth African Sugar Association Experiment StationKwaZulu NatalSouth Africa

Personalised recommendations