Alteration in the Expression of Compartmentation: in vitro Studies

  • D. D. Clarke
  • S. Berl


Glutamic acid and glutamine are present in unusually large concentrations in brain. The only known enzymatic mechanism which has been reported for the synthesis of glutamine is that catalysed by glutamine synthetase (E.C. These amino acids provide a very useful probe for studying precursor-product relationships in brain tissue in vivo. It is evident that the non-steady state situation presented by injection of a single dose of inhibitor followed by a tracer dose of a labelled precursor at some later time—a practice which has been used extensively in previous attempts to clarify the effects of metabolic inhibitors—makes it rather difficult to interpret the results. In vitro preparations are an attractive way to avoid some of these problems. In addition, there have been cases where a labelled precursor injected intravenously or intraperitoneally, for example alanine, was first metabolized to glucose with scrambling of the label before entering the brain (Koeppe and Hahn, 1962).


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  1. Baxter, C. F., and Roberts, E. (1961). J. Biol. Chem., 236, 3287–94.Google Scholar
  2. Berl, S., and Clarke, D. D. (1972). Brain Res., 36, 203–13. Lajtha, A. New York: Plenum.CrossRefGoogle Scholar
  3. Berl, S., and Clarke, D. D. (1972). Brain Res., 36, 203–13.CrossRefGoogle Scholar
  4. Berl, S., Clarke, D. D., and Nicklas, W. J. (1970). J. Neurochem., 17, 999–1007.CrossRefGoogle Scholar
  5. Berl, S., Nicklas, W. J., and Clarke, D. D. (1968). J. Neurochem., 15, 131–40.CrossRefGoogle Scholar
  6. Berl, S., Nicklas, W. J., and Clarke, D. D. (1970). J. Neurochem., 17, 1009–15.CrossRefGoogle Scholar
  7. Berl, S., Takagaki, G., Clarke, D. D., and Waelsch, H. (1962). J. Biol. Chem., 237, 2562–9.Google Scholar
  8. Brodie, J. D., and Nicholls, P. (1970). Biochim. Biophys. Acta, 198, 423–37.CrossRefGoogle Scholar
  9. Clarke, D. D., Nicklas, W. J., and Berl, S. (1970). Biochem. J., 120, 345–51.CrossRefGoogle Scholar
  10. Clarke, D. D., Nicklas, W. J., and Palumbo, J. (1968). Arch. Biochem. Biophys., 123, 205–6.CrossRefGoogle Scholar
  11. Cohen, M. M., Simon, G. R., Berry, J. F., and Chain, E. B. (1962). Biochem. J., 84, 43–4P.CrossRefGoogle Scholar
  12. Cremer, J. (1964). J. Neurochem., 11, 165–85.CrossRefGoogle Scholar
  13. Cremer, J. (1967). Biochem. J., 104, 223–8.CrossRefGoogle Scholar
  14. Goldberg, N. D., Passoneau, J. V., and Lowry, O. H. (1966). J. Biol. Chem., 241, 3997–4003.Google Scholar
  15. Haber, B. J. (1965). Can. J. Biochem., 43, 865–76.CrossRefGoogle Scholar
  16. Haslam, R. J., and Krebs, H. A. (1963). Biochem. J., 88, 566–77.CrossRefGoogle Scholar
  17. Koeppe, R. H., and Hahn, C. H. (1962). J. Biol. Chem., 237, 1026–8.Google Scholar
  18. Lahiri, S., and Quastel, J. H. (1963). Biochem. J., 89, 157–63.CrossRefGoogle Scholar
  19. McIlwain, H., and Rodnight, R. (1962). Practical Neurochemistry, 20, Boston: Little Brown.Google Scholar
  20. Neidle, A., Van den Berg, C., and Grynbaum, A. (1969). J. Neurochem., 16, 225–34.CrossRefGoogle Scholar
  21. Nicklas, W. J., Clarke, D. D., and Berl, S. (1969). J. Neurochem., 16, 549–58.CrossRefGoogle Scholar
  22. Nigh, W. G., and Richards, J. H. (1969). J. Amer. Chem. Soc., 91, 5847–8.CrossRefGoogle Scholar
  23. Okamoto, K., and Quastel, J. H. (1970). Biochem. J., 120, 37–47.CrossRefGoogle Scholar
  24. Peters, R. A. (1957). Adv. Enzymol., 18, 113–59.Google Scholar
  25. Roa, P. D., Tews, J. K., and Stone, W. E. (1964). Biochem. Pharmacol, 13, 477–87.CrossRefGoogle Scholar
  26. Simon, G., Cohen, M. M., and Berry, J. F. (1968). Biochem. J., 107, 109–11.CrossRefGoogle Scholar
  27. Teipel, J. W., Hass, G. M., and Hill, R. L. (1968). J. Biol. Chem., 243, 5684–94.Google Scholar
  28. Terner, C., Eggleston, L. V., and Krebs, H. A. (1950). Biochem. J., 47, 139–49.CrossRefGoogle Scholar
  29. Tolman, V., and Veres, K. (1966). Tetrahedron Lett., 32, 3909.CrossRefGoogle Scholar
  30. Trachtenberg, M. C., and Pollen, D. A. (1970). Science, 167, 1248–52.CrossRefGoogle Scholar
  31. Waelsch, H., Berl, S., Rossi, C. A., Clarke, D. D., and Purpura, D. P. (1964). J. Neurochem., 11, 717–28.CrossRefGoogle Scholar
  32. Wallach, D. P. (1961a). Biochem. Pharmacol., 5, 166–7.CrossRefGoogle Scholar
  33. Wallach, D. P. (1961b). Biochem. Pharmacol., 8, 328–31.CrossRefGoogle Scholar
  34. Wilson, J. E., and Barch, D. (1971). Fed. Proc., 30, 1139.Google Scholar

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© The Contributors 1973

Authors and Affiliations

  • D. D. Clarke
  • S. Berl

There are no affiliations available

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