Proviral DNA Cloning

  • Julie Overbaugh
  • Steven Dewhurst
  • James I. Mullins

Abstract

There are two primary considerations when preparing to clone a retrovirus, particularly HIV. The first is choosing the source of viral DNA, and the second is selecting an appropriate cloning vector.

Keywords

Vortex Sucrose Phenol Agar EDTA 

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Copyright information

© Stockton Press 1990

Authors and Affiliations

  • Julie Overbaugh
    • 1
  • Steven Dewhurst
    • 2
  • James I. Mullins
    • 3
  1. 1.Department of MicrobiologyUniversity of WashingtonSeattleUSA
  2. 2.Department of Cancer BiologyHarvard School of Public HealthBostonUSA
  3. 3.Department of Microbiology and ImmunologyStanford University School of MedicineStanfordUSA

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