Light scattering immunoassay

  • Christopher Price
  • David Newman


Monitoring of the antigen:antibody reaction by measurement of light scattering has been known for the past 50 years (1), although the broader concept of agglutination had been explored in the 1920’s (2), for microbiological assays. As an understanding of light scattering theory and the nature of antigens and antibodies evolved, a whole range of developments began to unfold. These can broadly be considered to reflect improvements in antibody production, increasing sophistication of instrumentation, better knowledge of the reaction between antigen and antibody and immunoaggregate formation, and more recently the use of particle labels to enhance light scattering. Although we might now see a particle enhanced immunoassay as a modern development of light scattering immunoassay technology, some of the earliest documented immunoassays involved the use of antibodies coupled to particles (3,4).


Light Scattering Immune Complex Latex Particle Antigen Concentration Photon Correlation Spectroscopy 
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© Palgrave Macmillan, a division of Macmillan Publishers Limited 1991

Authors and Affiliations

  • Christopher Price
  • David Newman

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